LOCUS       sequence-437467-       14088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of CBPdeltaIDR7-GFP in mammalian cells by 
            retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14088)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 14088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRetroX-TRE3G_CBPdIDR7-GFP
FEATURES             Location/Qualifiers
     source          1..14088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        307..510
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             511..686
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    749..948
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1149..1565
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1495..1517
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1533..1549
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1605..1954
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1613..1631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1649..1667
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1685..1703
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1721..1739
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1757..1775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1793..1811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1829..1847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1893..1917
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6828..6837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8316..9035
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        9066..9565
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9101..9124)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9498..9517
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9586..10611
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9586..10611
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             10792..11217
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(11320..11342)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11478..11498)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11483..11812
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11663..11798
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11834..11853
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11934..11951)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12105..12693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12185..12204)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12864..13655,13656..13724))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13487..13506
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13725..13829)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13897..13915
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(14015..14088,1..306)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgccccacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct caataaaaga gcccacaacc cctcactcgg
      541 cgcgccagtc ttccgataga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt
      601 gctgtttgca tccgaatcgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
      661 actacccacg acgggggtct ttcatttggg ggctcgtccg ggatttggag acccctgccc
      721 agggaccacc gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg
      781 attgtctagt gtctatgttt gatgttatgc gcctgcgtct gtactagtta gctaactagc
      841 tctgtatctg gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg
      901 ggagacgtcc cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga
      961 tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt
     1021 tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc
     1081 tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg
     1141 aaaattaggg ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg
     1201 tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct
     1261 gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag
     1321 acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc
     1381 aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc
     1441 cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg
     1501 aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag
     1561 gcgccggaat tgaagatctc gatgaggccc tttcgtcttc actcgagttt actccctatc
     1621 agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg tatgcagact
     1681 ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag tgatagagaa
     1741 cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt actccctatc
     1801 agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg tatgtcgagg
     1861 taggcgtgta cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc
     1921 tggagcaatt ccacaacact tttgtcttat acttggatcc aatggctgag aacttgctgg
     1981 acggaccgcc caaccccaaa agagccaaac tcagctcgcc cggtttctcg gcgaatgaca
     2041 gcacagattt tggatcattg tttgacttgg aaaatgatct tcctgatgag ctgataccca
     2101 atggaggaga attaggcctt ttaaacagtg ggaaccttgt tccagatgct gcttccaaac
     2161 ataaacaact gtcggagctt ctacgaggag gcagcggctc tagtatcaac ccaggaatag
     2221 gaaatgtgag cgccagcagc cccgtgcagc agggcctggg tggccaggct caagggcagc
     2281 cgaacagtgc taacatggcc agcctcagtg ccatgggcaa gagccctctg agccagggag
     2341 attcttcagc ccccagcctg cctaaacagg cagccagcac ctctgggccc acccccgctg
     2401 cctcccaagc actgaatccg caagcacaaa agcaagtggg gctggcgact agcagccctg
     2461 ccacgtcaca gactggacct ggtatctgca tgaatgctaa ctttaaccag acccacccag
     2521 gcctcctcaa tagtaactct ggccatagct taattaatca ggcttcacaa gggcaggcgc
     2581 aagtcatgaa tggatctctt ggggctgctg gcagaggaag gggagctgga atgccgtacc
     2641 ctactccagc catgcagggc gcctcgagca gcgtgctggc tgagacccta acgcaggttt
     2701 ccccgcaaat gactggtcac gcgggactga acaccgcaca ggcaggaggc atggccaaga
     2761 tgggaataac tgggaacaca agtccatttg gacagccctt tagtcaagct ggagggcagc
     2821 caatgggagc cactggagtg aacccccagt tagccagcaa acagagcatg gtcaacagtt
     2881 tgcccacctt ccctacagat atcaagaata cttcagtcac caacgtgcca aatatgtctc
     2941 agatgcaaac atcagtggga attgtaccca cacaagcaat tgcaacaggc cccactgcag
     3001 atcctgaaaa acgcaaactg atacagcagc agctggttct actgcttcat gctcataagt
     3061 gtcagagacg agagcaagca aacggagagg ttcgggcctg ctcgctcccg cattgtcgaa
     3121 ccatgaaaaa cgttttgaat cacatgacgc attgtcaggc tgggaaagcc tgccaagttg
     3181 cccattgtgc atcttcacga caaatcatct ctcattggaa gaactgcaca cgacatgact
     3241 gtcctgtttg cctccctttg aaaaatgcca gtgacaagcg aaaccaacaa accatcctgg
     3301 ggtctccagc tagtggaatt caaaacacaa ttggttctgt tggcacaggg caacagaatg
     3361 ccacttcttt aagtaaccca aatcccatag accccagctc catgcagcga gcctatgctg
     3421 ctctcggact cccctacatg aaccagcccc agacgcagct gcagcctcag gttcctggcc
     3481 agcaaccagc acagcctcaa acccaccagc agatgaggac tctcaacccc ctgggaaata
     3541 atccaatgaa cattccagca ggaggaataa caacagatca gcagccccca aacttgattt
     3601 cagaatcagc tcttccgact tccctggggg ccacaaaccc actgatgaac gatggctcca
     3661 actctggtaa cattggaacc ctcagcacta taccaacagc agctcctcct tctagcaccg
     3721 gtgtaaggaa aggctggcac gaacatgtca ctcaggacct gcggagccat ctagtgcata
     3781 aactcgtcca agccatcttc ccaacacctg atcccgcagc tctaaaggat cgccgcatgg
     3841 aaaacctggt agcctatgct aagaaagtgg aaggggacat gtacgagtct gccaacagca
     3901 gggatgaata ttatcactta ttagcagaga aaatctacaa gatacaaaaa gaactagaag
     3961 aaaaacggag gtcgcgttta cataaacaag gcatcttggg gaaccagcca gccttaccag
     4021 ccccgggggc tcagccccct gtgattccac aggcacaacc tgtgagacct ccaaatggac
     4081 ccctgtccct gccagtgaat cgcatgcaag tttctcaagg gatgaattca tttaacccca
     4141 tgtccttggg gaacgtccag ttgccacaag cacccatggg acctcgtgca gcctccccaa
     4201 tgaaccactc tgtccagatg aacagcatgg gctcagtgcc agggatggcc atttctcctt
     4261 cccgaatgcc tcagcctccg aacatgatgg gtgcacacac caacaacatg atggcccagg
     4321 cgcccgctca gagccagttt ctgccacaga accagttccc gtcatccagc ggggcgatga
     4381 gtgtgggcat ggggcagccg ccagcccaaa caggcgtgtc acagggacag gtgcctggtg
     4441 ctgctcttcc taaccctctc aacatgctgg ggcctcaggc cagccagcta ccttgccctc
     4501 cagtgacaca gtcaccactg cacccaacac cgcctcctgc ttccacggct gctggcatgc
     4561 catctctcca gcacacgaca ccacctggga tgactcctcc ccagccagca gctcccactc
     4621 agccatcaac tcctgtgtcg tcttccgggc agactcccac cccgactcct ggctcagtgc
     4681 ccagtgctac ccaaacccag agcaccccta cagtccaggc agcagcccag gcccaggtga
     4741 ccccgcagcc tcaaacccca gttcagcccc cgtctgtggc tacccctcag tcatcgcagc
     4801 aacagccgac gcctgtgcac gcccagcctc ctggcacacc gctttcccag gcagcagcca
     4861 gcattgataa cagagtccct accccctcct cggtggccag cgcagaaacc aattcccagc
     4921 agccaggacc tgacgtacct gtgctggaaa tgaagacgga gacccaagca gaggacactg
     4981 agcccgatcc tggtgaatcc aaaggggagc ccaggtctga gatgatggag gaggatttgc
     5041 aaggagcttc ccaagttaaa gaagaaacag acatagcaga gcagaaatca gaaccaatgg
     5101 aagtggatga aaagaaacct gaagtgaaag tagaagttaa agaggaagaa gagagtagca
     5161 gtaacggcac agcctctcag tcaacatctc cttcgcagcc gcgcaaaaaa atctttaaac
     5221 cagaggagtt acgccaggcc ctcatgccaa ccctagaagc actgtatcga caggacccag
     5281 agtcattacc tttccggcag cctgtagatc cccagctcct cggaattcca gactattttg
     5341 acatcgtaaa gaatcccatg gacctctcca ccatcaagcg gaagctggac acagggcaat
     5401 accaagagcc ctggcagtac gtggacgacg tctggctcat gttcaacaat gcctggctct
     5461 ataatcgcaa gacatcccga gtctataagt tttgcagtaa gcttgcagag gtctttgagc
     5521 aggaaattga ccctgtcatg cagtcccttg gatattgctg tggacgcaag tatgagtttt
     5581 ccccacagac tttgtgctgc tatgggaagc agctgtgtac cattcctcgc gatgctgcct
     5641 actacagcta tcagaatagg tatcatttct gtgagaagtg tttcacagag atccagggcg
     5701 agaatgtgac cctgggtgac gacccttcac agccccagac gacaatttca aaggatcagt
     5761 ttgaaaagaa gaaaaatgat accttagacc ccgaaccttt cgttgattgc aaggagtgtg
     5821 gccggaagat gcatcagatt tgcgttctgc actatgacat catttggcct tcaggttttg
     5881 tgtgcgacaa ctgcttgaag aaaactggca gacctcgaaa agaaaacaaa ttcagtgcta
     5941 agaggctgca gaccacaaga ctgggaaacc acttggaaga ccgagtgaac aaatttttgc
     6001 ggcgccagaa tcaccctgaa gccggggagg tttttgtccg agtggtggcc agctcagaca
     6061 agacggtgga ggtcaagccc gggatgaagt cacggtttgt ggattctggg gaaatgtctg
     6121 aatctttccc atatcgaacc aaagctctgt ttgcttttga ggaaattgac ggcgtggatg
     6181 tctgcttttt tggaatgcac gtccaagaat acggctctga ttgcccccct ccaaacacga
     6241 ggcgtgtgta catttcttat ctggatagta ttcatttctt ccggccacgt tgcctccgca
     6301 cagccgttta ccatgagatc cttattggat atttagagta tgtgaagaaa ttagggtatg
     6361 tgacagggca catctgggcc tgtcctccaa gtgaaggaga tgattacatc ttccattgcc
     6421 acccacctga tcaaaaaata cccaagccaa aacgactgca ggagtggtac aaaaagatgc
     6481 tggacaaggc gtttgcagag cggatcatcc atgactacaa ggatattttc aaacaagcaa
     6541 ctgaagacag gctcaccagt gccaaggaac tgccctattt tgaaggtgat ttctggccca
     6601 atgtgttaga agagagcatt aaggaactag aacaagaaga agaggagagg aaaaaggaag
     6661 agagcactgc agccagtgaa accactgagg gcagtcaggg cgacagcaag aatgccaaga
     6721 agaagaacaa caagaaaacc aacaagaaca aaagcagcat cagccgcgcc aacaagaaga
     6781 agcccagcat gcccaacgtg tccaatgacc tgtcccagaa gctgtatgcc accatggaga
     6841 agcacaagga ggtcttcttc gtgatccacc tgcacgctgg gcctgtcatc aacaccctgc
     6901 cccccatcgt cgaccccgac cccctgctca gctgtgacct catggatggg cgcgacgcct
     6961 tcctcaccct cgccagagac aagcactggg agttctcctc cttgcgccgc tccaagtggt
     7021 ccacgctctg catgctggtg gagctgcaca cccagggcca ggaccgcttt gtctacacct
     7081 gcaacgagtg caagcaccac gtggagacgc gctggcactg cactgtgtgc gaggactacg
     7141 acctctgcat caactgctat aacacgaaga gccatgccca taagatggtg aagtgggggc
     7201 tgggcctgga tgacgagggc agcagccagg gcgagccaca gtcaaagagc ccccaggagt
     7261 cacgccggct gagcatccag cgctgcatcc agtcgctggt gcacgcgtgc cagtgccgca
     7321 acgccaactg ctcgctgcca tcctgccaga agatgaagcg ggtggtgcag cacaccaagg
     7381 gctgcaaacg caagaccaac gggggctgcc cggtgtgcaa gcagctcatc gccctctgct
     7441 gctaccacgc caagcactgc caagaaaaca aatgccccgt gcccttctgc ctcaacatca
     7501 aacacaagct ccgccagcag cagatccagc accgcctgca gcaggcccag ctcatgcgcc
     7561 ggcggatggc caccatgaac acccgcaacg tgcctcagca gagtctgcct tctcctacct
     7621 cagcaccgcc cgggaccccc acacagcagc ccagcacacc ccagacgccg cagccccctg
     7681 cccagcccca accctcaccc gtgagcatgt caccagctgg cttccccagc gtggcccgga
     7741 ctcagccccc caccacggtg tccacaggga agcctaccag ccaggtgccg gcccccccac
     7801 ccccggccca gccccctcct gcagcggtgg aagcggctcg gcagatcgag cgtgaggccc
     7861 agcagcagca gcacctgtac cgggtgaaca tcaacaacag catgccccca ggacgcacgg
     7921 gcatggggac cccggggagc cagatggccc ccgtgagcct gaatgtgccc cgacccaacc
     7981 aggtgagcgg gcccgtcatg cccagcatgc ctcccgggca gtggcagcag gcgccccttc
     8041 cccagcagca gcccatgcca ggcttgccca ggcctgtgat atccatgcag gcccaggcgg
     8101 ccgtggctgg gccccggatg cccagcgtgc agccacccag gagcatctca cccagcgctc
     8161 tgcaagacct gctgcggacc ctgaagtcgc ccagctcccc tcagcagcaa cagcaggtgc
     8221 tgaacattct caaatcaaac ccgcagctaa tggcagcttt catcaaacag cgcacagcca
     8281 agtacctaga gaagtttgtg gagggcttgc cggtcatggt gagcaagggc gccgagctgt
     8341 tcaccggcat cgtgcccatc ctgatcgagc tgaatggcga tgtgaatggc cacaagttca
     8401 gcgtgagcgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     8461 gcaccaccgg caagctgcct gtgccctggc ccaccctggt gaccaccctg agctacggcg
     8521 tgcagtgctt ctcacgctac cccgatcaca tgaagcagca cgacttcttc aagagcgcca
     8581 tgcctgaggg ctacatccag gagcgcacca tcttcttcga ggatgacggc aactacaagt
     8641 cgcgcgccga ggtgaagttc gagggcgata ccctggtgaa tcgcatcgag ctgaccggca
     8701 ccgatttcaa ggaggatggc aacatcctgg gcaataagat ggagtacaac tacaacgccc
     8761 acaatgtgta catcatgacc gacaaggcca agaatggcat caaggtgaac ttcaagatcc
     8821 gccacaacat cgaggatggc agcgtgcagc tggccgacca ctaccagcag aataccccca
     8881 tcggcgatgg ccctgtgctg ctgcccgata accactacct gtccacccag agcgccctgt
     8941 ccaaggaccc caacgagaag cgcgatcaca tgatctactt cggcttcgtg accgccgccg
     9001 ccatcaccca cggcatggat gagctgtaca agtgagcggc cgcgccggcg ggcccgaatt
     9061 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     9121 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     9181 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     9241 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     9301 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     9361 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     9421 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     9481 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     9541 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgaa aaagcctgaa
     9601 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     9661 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     9721 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     9781 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     9841 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     9901 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     9961 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
    10021 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
    10081 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
    10141 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
    10201 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
    10261 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
    10321 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
    10381 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
    10441 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
    10501 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
    10561 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcgcccgccc
    10621 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcggc gtctcgagat
    10681 atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta
    10741 cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
    10801 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa
    10861 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagggtcgac
    10921 cctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
    10981 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
    11041 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
    11101 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
    11161 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg
    11221 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta
    11281 agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    11341 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    11401 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
    11461 agtgtagatc cggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
    11521 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
    11581 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
    11641 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    11701 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    11761 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac
    11821 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
    11881 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
    11941 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    12001 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    12061 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    12121 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    12181 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    12241 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    12301 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    12361 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    12421 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    12481 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    12541 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    12601 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    12661 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    12721 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    12781 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    12841 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    12901 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    12961 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    13021 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    13081 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    13141 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
    13201 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    13261 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    13321 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    13381 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    13441 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
    13501 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    13561 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    13621 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    13681 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    13741 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    13801 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    13861 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    13921 cgtcttcaag aattagcttg gccattgcat acgttgtatc catatcataa tatgtacatt
    13981 tatattggct catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa
    14041 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttc
//
