LOCUS       sequence-437707-       11565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CBP autoregulatory loop (AL) fragment fused to 
            optogenetic protein mCh-Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11565)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR-AL-mCh-Cry2WT-NLS
FEATURES             Location/Qualifiers
     source          1..11565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1747..3240
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3337..3357
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3422..4010
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3475..3495)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3893..3904)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4013..4029)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4014..4030)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4120..4353
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4378..4396)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4379..4396)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4686..4705)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4919..4938)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5038..5060
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5098..5116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5184..5288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5289..5357,5358..6149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5507..5526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6320..6908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6809..6828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7062..7079
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7149..7169)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7154..7483
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7334..7469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8617..8682
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8812..8832
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9257..9391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9294..9313)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9348..9367
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9559..10192
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10239..10364
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10861..11094
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11279..11323
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg gacaggctca ccagtgccaa ggaactgccc
      781 tattttgaag gtgatttctg gcccaatgtg ttagaagaga gcattaagga actagaacaa
      841 gaagaagagg agaggaaaaa ggaagagagc actgcagcca gtgaaaccac tgagggcagt
      901 cagggcgaca gcaagaatgc caagaagaag aacaacaaga aaaccaacaa gaacaaaagc
      961 agcatcagcc gcgccaacaa gaagaagagt ggagcaaggc aggtggacag tggatccgga
     1021 gctaccatgg tgtctaaagg cgaggaggat aacatggcta taatcaagga atttatgagg
     1081 ttcaaggtgc atatggaggg atctgtgaac ggtcacgagt tcgaaatcga gggcgaaggc
     1141 gaggggcgac cctatgaagg gacacagacg gccaaactga aggtgaccaa gggtggcccc
     1201 ctgcctttcg cctgggacat cctgtcaccc cagtttatgt acggctctaa ggcttacgtt
     1261 aagcaccctg ccgatattcc ggactacttg aaactgagct tccctgaagg gttcaagtgg
     1321 gaacgggtaa tgaattttga ggatggcggt gtagtcacag ttacccagga cagctctctt
     1381 caggacggag aatttatcta taaggttaaa cttcggggca ctaacttccc atcagacggc
     1441 cccgtgatgc agaagaaaac tatgggctgg gaagccagct cagaacgcat gtatcccgag
     1501 gacggagcac tgaagggaga aatcaagcag cgactcaagc tgaaggatgg gggacattat
     1561 gatgctgagg tcaaaaccac ctataaggcc aagaaacccg ttcagcttcc tggggcatac
     1621 aacgtgaata tcaaactgga tattacatcc cataacgaag actataccat cgtggaacag
     1681 tacgagcggg ccgagggcag acatagcaca gggggcatgg atgaattgta caaggcggcc
     1741 acgcgtatga agatggacaa aaagactata gtttggttta gaagagacct aaggattgag
     1801 gataatcctg cattagcagc agctgctcac gaaggatctg tttttcctgt cttcatttgg
     1861 tgtcctgaag aagaaggaca gttttatcct ggaagagctt caagatggtg gatgaaacaa
     1921 tcacttgctc acttatctca atccttgaag gctcttggat ctgacctcac tttaatcaaa
     1981 acccacaaca cgatttcagc gatcttggat tgtatccgcg ttaccggtgc tacaaaagtc
     2041 gtctttaacc acctctatga tcctgtttcg ttagttcggg accataccgt aaaggagaag
     2101 ctggtggaac gtgggatctc tgtgcaaagc tacaatggag atctattgta tgaaccgtgg
     2161 gagatatact gcgaaaaggg caaacctttt acgagtttca attcttactg gaagaaatgc
     2221 ttagatatgt cgattgaatc cgttatgctt cctcctcctt ggcggttgat gccaataact
     2281 gcagcggctg aagcgatttg ggcgtgttcg attgaagaac tagggctgga gaatgaggcc
     2341 gagaaaccga gcaatgcgtt gttaactaga gcttggtctc caggatggag caatgctgat
     2401 aagttactaa atgagttcat cgagaagcag ttgatagatt atgcaaagaa cagcaagaaa
     2461 gttgttggga attctacttc actactttct ccgtatctcc atttcgggga aataagcgtc
     2521 agacacgttt tccagtgtgc ccggatgaaa caaattatat gggcaagaga taagaacagt
     2581 gaaggagaag aaagtgcaga tctttttctt aggggaatcg gtttaagaga gtattctcgg
     2641 tatatatgtt tcaacttccc gtttactcac gagcaatcgt tgttgagtca tcttcggttt
     2701 ttcccttggg atgctgatgt tgataagttc aaggcctgga gacaaggcag gaccggttat
     2761 ccgttggtgg atgccggaat gagagagctt tgggctaccg gatggatgca taacagaata
     2821 agagtgattg tttcaagctt tgctgtgaag tttcttctcc ttccatggaa atggggaatg
     2881 aagtatttct gggatacact tttggatgct gatttggaat gtgacatcct tggctggcag
     2941 tatatctctg ggagtatccc cgatggccac gagcttgatc gcttggacaa tcccgcgtta
     3001 caaggcgcca aatatgaccc agaaggtgag tacataaggc aatggcttcc cgagcttgcg
     3061 agattgccaa ctgaatggat ccatcatcca tgggacgctc ctttaaccgt actcaaagct
     3121 tctggtgtgg aactcggaac aaactatgcg aaacccattg tagacatcga cacagctcgt
     3181 gagctactag ctaaagctat ttcaagaacc cgtgaagcac agatcatgat cggagcagca
     3241 gcccgggatc caccggtcgc caccggttca gggagcggat ccggctcagg gagtgcggcc
     3301 gcaactccca cctgcaacat gcgtgacgga ggcggtccaa aaaagaagag aaaggtatga
     3361 ctgaggccgc gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga
     3421 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3481 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3541 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3601 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3661 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3721 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3781 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3841 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3901 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3961 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     4021 tcgacctcga gggaattaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     4081 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4141 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     4201 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4261 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4321 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct
     4381 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
     4441 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
     4501 atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc
     4561 tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
     4621 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     4681 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     4741 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     4801 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     4861 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
     4921 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     4981 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     5041 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5101 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5161 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5221 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5281 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5341 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5401 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5461 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5521 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5581 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5641 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5701 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5761 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5821 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5881 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5941 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6001 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6061 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6121 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6181 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6241 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6301 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6361 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6421 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6481 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6541 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6601 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6661 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6721 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6781 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6841 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6901 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6961 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7021 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7081 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7141 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7201 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7261 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7321 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7381 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7441 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
     7501 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     7561 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
     7621 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     7681 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     7741 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     7801 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
     7861 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
     7921 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
     7981 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
     8041 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
     8101 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
     8161 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
     8221 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
     8281 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
     8341 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
     8401 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
     8461 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
     8521 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     8581 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     8641 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     8701 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     8761 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     8821 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     8881 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     8941 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
     9001 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
     9061 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
     9121 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
     9181 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     9241 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     9301 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9361 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     9421 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     9481 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     9541 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     9601 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     9661 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     9721 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     9781 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     9841 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     9901 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     9961 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
    10021 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    10081 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    10141 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
    10201 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
    10261 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
    10321 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
    10381 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
    10441 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
    10501 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
    10561 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
    10621 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
    10681 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
    10741 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
    10801 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
    10861 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
    10921 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
    10981 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
    11041 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    11101 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    11161 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    11221 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    11281 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    11341 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    11401 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    11461 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
    11521 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaa
//
