LOCUS       sequence-437751-       10181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of prime editor PEMax (Plasmid #174820) only in cells 
            with TDP-43 loss of function. Note: It is not recommended to produce
            lentivirus containing TDP-REG sequences – see Depositor Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Wilkins OG, Chien MZYJ, Wlaschin JJ, Barattucci S, Harley P, Mattedi
            F, Mehta PR, Pisliakova M, Ryadnov E, Keuss MJ, Thompson D, Digby H,
            Knez L, Simkin RL, Diaz JA, Zanovello M, Brown AL, Darbey A, Karda 
            R, Fisher EMC, Cunningham TJ, Le Pichon CE, Ule J, Fratta P
  TITLE     Creation of de novo cryptic splicing for ALS and FTD precision 
            medicine.
  JOURNAL   Science. 2024 Oct 4;386(6717):61-69. doi: 10.1126/science.adk2539. 
            Epub 2024 Oct 3.
  PUBMED    39361759
REFERENCE   2  (bases 1 to 10181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adk2539"; journalName: "Science"; date: "2024-10-4-
            4"; volume: "386"; issue: "6717"; pages: "61-69"
COMMENT     Sequence Label: TDP-REGv2 Cryptic PE-Max
FEATURES             Location/Qualifiers
     source          1..10181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..433
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        434..637
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     588..608
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     679..698
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        679..697
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             747..767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             768..2483
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5312..5332
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7433..7453
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7463..7489
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7498..7515
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7538..7555)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7544..7768
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7839..7855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7839..7855)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7852..7874)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7863..7879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7887..7893,7894..7911,7912..7917))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7932..7953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8070..8087)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8241..8829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8321..8340)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9000..9791,9792..9860))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9623..9642
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9861..9965)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10044..10063)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
       61 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      121 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      181 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      241 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      301 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      361 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      421 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      481 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      541 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      601 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
      661 ctagagatcc gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga
      721 cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca
      781 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      841 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      901 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
      961 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     1021 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     1081 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     1141 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     1201 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     1261 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     1321 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     1381 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagaaagctg gaaaatctga
     1441 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     1501 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     1561 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     1621 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     1681 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     1741 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     1801 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     1861 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     1921 ccgaggaact gctcgtgaag ctgaagagag aggacctgct gcggaagcag cggaccttcg
     1981 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     2041 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     2101 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     2161 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     2221 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     2281 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     2341 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     2401 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     2461 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     2521 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     2581 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     2641 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     2701 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggtaa gaatgcacat
     2761 cacttcttga gagtatggag gagtgaaatg acactcagtg ccagagttac tgtatatcta
     2821 cactttaaaa gtgtagcttt taaaagataa gcaagcacaa tcttttgtgt gtgtgtgtgt
     2881 gaatgtgtgt gtgtgtgtgt gtcacccaga ctgtcccgta agctcatcaa tggcatacgc
     2941 gataaacaat cggggaagac aatcctggat tttcttaaat ctgatggatt tgcgaatcgt
     3001 aactttatgc agctgatcca tgatgactcc ctgacgttca aagaggatat tcagaaggcg
     3061 caggtaagta tcgtgtgtgt gttttttttt gtatttttta ccgagtcggg gtttcgcaat
     3121 gttgcccagg ctggtctcag agtctcgctc tgttgtctac gctggagtgc agtaacatga
     3181 gccactgtgc ccggccaatc ctaagaattt cttttgcggt ggttgcaagt ctgggcagaa
     3241 ctcttgtcag gggctgtaac tggacttatc tttactcctt tgtcaggtat ccggccaggg
     3301 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
     3361 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
     3421 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
     3481 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
     3541 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
     3601 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
     3661 tgtggacgct atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct
     3721 gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
     3781 gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt
     3841 cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat
     3901 caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc
     3961 ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac
     4021 cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga
     4081 gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct
     4141 gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga
     4201 cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt
     4261 cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat
     4321 ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg
     4381 ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa
     4441 gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga
     4501 taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc
     4561 caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact
     4621 gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa
     4681 tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa
     4741 gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc
     4801 cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta
     4861 cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct
     4921 gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc
     4981 caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca
     5041 ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa
     5101 tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac
     5161 cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga
     5221 gacacggatc gacctgtctc agctgggagg tgactccggc ggaagctctg gtggcagcaa
     5281 gcggaccgcc gacggctctg aattcgagag ccctaagaag aaaagaaagg tgagcggagg
     5341 ctctagcggc ggaagcaccc tgaacattga agacgagtat agactgcatg aaacaagcaa
     5401 ggaacccgac gtgtccctgg gctccacctg gctgtccgac tttccccagg cctgggccga
     5461 gacaggagga atgggcctgg ccgtgcggca ggcacccctg atcatccctc tgaaggccac
     5521 ctctacaccc gtgagcatca agcagtaccc tatgtctcag gaggccagac tgggcatcaa
     5581 gcctcacatc cagaggctgc tggaccaggg catcctggtg ccatgccaga gcccctggaa
     5641 cacaccactg ctgcccgtga agaagccagg caccaatgac tatagacccg tgcaggatct
     5701 gagagaggtg aacaagaggg tggaggatat ccaccccacc gtgcccaacc cttacaatct
     5761 gctgtccggc ctgccccctt ctcaccagtg gtatacagtg ctggacctga aggatgcctt
     5821 cttttgtctg agactgcacc ctaccagcca gccactgttc gcctttgagt ggagggaccc
     5881 tgagatgggc atctctggcc agctgacctg gacacgcctg cctcagggct tcaagaatag
     5941 cccaacactg tttaacgagg ccctgcaccg cgacctggca gatttccgga tccagcaccc
     6001 agatctgatc ctgctgcagt acgtggacga tctgctgctg gccgccacca gcgagctgga
     6061 ttgccagcag ggaacacgcg ccctgctgca gaccctggga aacctgggat atagggcatc
     6121 cgccaagaag gcccagatct gtcagaagca ggtgaagtac ctgggctatc tgctgaagga
     6181 gggccagaga tggctgacag aggccaggaa ggagacagtg atgggccagc caacacccaa
     6241 gaccccaaga cagctgaggg agttcctggg caaagcagga ttttgcaggc tgttcatccc
     6301 aggattcgca gagatggcag cacctctgta cccactgacc aagccgggca ccctgtttaa
     6361 ttggggccct gaccagcaga aggcctatca ggagatcaag caggccctgc tgacagcacc
     6421 agccctgggc ctgccagacc tgaccaagcc tttcgagctg tttgtggatg agaagcaggg
     6481 ctacgccaag ggcgtgctga cccagaagct gggaccatgg agacggcccg tggcctatct
     6541 gtccaagaag ctggacccag tggcagcagg atggccacca tgcctgagga tggtggcagc
     6601 aatcgccgtg ctgacaaagg atgccggcaa gctgaccatg ggacagccac tggtcatcct
     6661 ggcaccacac gcagtggagg ccctggtgaa gcagcctcca gatcgctggc tgtctaacgc
     6721 ccggatgaca cactaccagg ccctgctgct ggacaccgat cgcgtgcagt ttggccctgt
     6781 ggtggccctg aatccagcca ccctgctgcc tctgccagag gagggcctgc agcacaactg
     6841 tctggacatc ctggcagagg cacacggaac aaggccagac ctgaccgatc agcccctgcc
     6901 tgacgccgat cacacatggt ataccgatgg aagctccctg ctgcaggagg gccagaggaa
     6961 ggcaggagca gcagtgacca cagagacaga agtgatctgg gccaaggccc tgccagcagg
     7021 cacatccgcc cagcgggccg agctgatcgc cctgacccag gccctgaaga tggccgaggg
     7081 caagaagctg aacgtgtaca cagactccag atatgccttc gccaccgcac acatccacgg
     7141 agagatctac aggcgccggg gctggctgac ctctgagggc aaggagatca agaacaagga
     7201 tgagatcctg gccctgctga aggccctgtt tctgcccaag cggctgagca tcatccactg
     7261 tcctggacac cagaagggac actccgccga ggcaaggggc aatcggatgg ccgaccaggc
     7321 cgccagaaag gctgctatta ctgaaactcc cgacacttcc actctgctga ttgaaaactc
     7381 ctccccttct ggcggctcaa aaagaaccgc cgacggcagc gaattcgagt ctcccaagaa
     7441 gaagaggaaa gtcggctctg gccctgccgc taagagagtg aagctggact aaccggtcat
     7501 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     7561 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7621 actgtccttt cctaataaaa tgagaaaatt gcatcgcatt gtctgagtag gtgtcattct
     7681 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7741 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     7801 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7861 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7921 tagggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7981 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8041 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8101 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8161 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8221 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8281 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8341 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8401 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8461 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8521 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8581 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8641 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8701 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8761 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8821 ggatctcaag aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac
     8881 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8941 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9001 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9061 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9121 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9181 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9241 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9301 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9361 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9421 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9481 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9541 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9601 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9661 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9721 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9781 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9841 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9901 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9961 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc
    10021 gatcccctag ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
    10081 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
    10141 acaaggcaag gcttgaccga caattgcatg aagaatctgc t
//
