LOCUS       sequence-437870-       11368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding 
            sequence under EF1A promotor and b. GFP reporter under UbC promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Haimovich G, Dasgupta S, Ravi AG, Gerst JE
  TITLE     Complementation of a human disease phenotype in vitro by 
            intercellular mRNA transfer
  JOURNAL   bioRxiv 2024.11.06.622258
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.06.622258"
COMMENT     Sequence Label: pLenti-Cre-MS2_UbC-GFP (Cre_GFP)
FEATURES             Location/Qualifiers
     source          1..11368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     misc_RNA        complement(444..462)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(483..501)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(553..571)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(592..610)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(662..680)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(701..719)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(771..789)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(810..828)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(880..898)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(919..937)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(989..1007)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1059..1077)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1098..1116)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1168..1186)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1238..1256)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1277..1295)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1347..1365)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1386..1404)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1456..1474)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1526..1544)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1565..1583)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             complement(1677..2705)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVVRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLQD
                     GD"
     CDS             complement(2706..2726)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(2742..3920)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2751..3689
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(2774..2794)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3986..4384
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             join(4395..4397,4398..4400,4401..5114)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4395..4397,4398..4400,4401..5111)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4701..4720)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5048..5069
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5130..5718
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5183..5203)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5601..5612)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5805..6038
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6110..6231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6147..6166)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6201..6220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6271..6406
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6426..6445)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6427..6445)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6455..6472)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6455..6471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6464..6486)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6613..7068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6700..6719)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6910..6931
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7094..7198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7199..7267,7268..8059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7417..7436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8230..8818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8719..8738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8972..8989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9106..9127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9142..9147,9148..9165,9166..9172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9180..9196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9185..9207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9204..9220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9204..9220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9239..9259
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9241..9259
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9287..9513
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9514..9694
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9741..9866
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10359..10592
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10777..10821
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11088..11205
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc
      361 caccaccttc tgataggcag cctgcacctg aggagtgaag gatccgatcg cgcgcagatc
      421 taatgaaccc gggaatactg cagacatggg tgatcctcat gttttctgga gtcgacctgc
      481 agacatgggt gatcctcatg ttttctaggc aattaggtac cttaggatct aatgaacccg
      541 ggaatactgc agacatgggt gatcctcatg ttttctagag tcgacctgca gacatgggtg
      601 atcctcatgt tttctaggca attaggtacc ttaggatcta atgaacccgg gaatactgca
      661 gacatgggtg atcctcatgt tttctagagt cgacctgcag acatgggtga tcctcatgtt
      721 ttctaggcaa ttaggtacct taggatctaa tgaacccggg aatactgcag acatgggtga
      781 tcctcatgtt ttctggagtc gacctgcaga catgggtgat cctcatgttt kctaggcaat
      841 taggtrcctt argatctaat gaacccggga atactgcaga catgggtgat cctcatgttt
      901 tctagagtcg acctgcagac atgggtgatc ctcatgtttt ctaggcaatt aggtacctta
      961 ggatctaatg aacccgggaa tactgcagac atgggtgatc ctcatgtttt ctaggcaatt
     1021 aggtacctta ggatctaatg aacccgggaa tactgcagac atgggtgatc ctcatgtttt
     1081 ctggagtcga cctgcagaca tgggtgatcc tcatgttttc taggcaakta ggtaccttag
     1141 gatctaatga mcscgggmat mctgcagaca tgggtgatcc tcatgttttc taggcaatta
     1201 ggtaccttag gatctaatga acccgggaat actgcagaca tgggtgatcc tcatgttttc
     1261 tggagtcgac ctgcagacat gggtgatcct catgttttct aggcaattag gtaccttagg
     1321 atctaatgaa cccgggaata ctgcagacat gggtgatcct catgttttct agagtcgacc
     1381 tgcagacatg ggtgatcctc atgttttcta ggcaattagg taccttagga tctaatgaac
     1441 ccgggaatac tgcagacatg ggtgatcctc atgttttcta ggcaattagg taccttagga
     1501 tctaatgaac ccgggaatac tgcagacatg ggtgatcctc atgttttctg gagtcgacct
     1561 gcagacatgg gtgatcctca tgttttctag gcaattaggt accttaggat cgatatatag
     1621 ggcccgaatt ctgcagatat ccagcacagt ggcggccgcg gcttgatcga aagcttctaa
     1681 tcgccatctt gcagcaggcg caccattgcc cctgtttcac tatccaggtt acggatatag
     1741 ttcatgacaa tatttacatt ggtccagcca ccagcttgca tgatctccgg tattgaaact
     1801 ccagcgcggg ccatatctcg cgcggctccg acacgggcac tgtgtccaga ccaggccagg
     1861 tatctctgac cagagtcatc cttagcgccg taaatcaatc gatgagttgc ttcaaaaatc
     1921 ccttccaggg cgcgagttga tagctggctg gtggcagatg gcgcggcaac accatttttt
     1981 ctgacccggc aaaacaggta gttattcgga tcatcagcta caccagagac ggaaatccat
     2041 cgctcgacca gtttagttac ccccaggcta agtgccttct ctacacctgc ggtgctaacc
     2101 agcgttttcg ttctgccaat atggattaac attctcccac cgtcagtacg tgagatatct
     2161 ttaaccctga tcctggcaat ttcggctata cgtaacaggg tgttataagc aatccccaga
     2221 aatgccagat tacgtatatc ctggcagcga tcgctatttt ccatgagtga acgaacctgg
     2281 tcgaaatcag tgcgttcgaa cgctagagcc tgttttgcac gttcaccggc atcaacgttt
     2341 tcttttcgga tccgccgcac aaccagtgaa acagcattgc tgtcacttgg tcgtggcagc
     2401 ccggaccgac gatgaagcat gtttagctgg cccaaatgtt gctggatagt ttttactgcc
     2461 agaccgcgcg cctgaagata tagaagataa tcgcgaacat cttcaggttc tgcgggaaac
     2521 catttccggt tattcaactt gcaccatgcc gcccacgacc ggcaaacgga cagaagcatt
     2581 ttccaggtat gctcagaaaa cgcctggcga tccctgaaca tgtccatcag gttcttgcga
     2641 acctcatcac tcgttgcatc gaccggtaat gcaggcaaat tttggtgtac ggtcagtaaa
     2701 ttggacacct tcctcttctt cttgggtgcc atgcggccgc ttcacgacac ctgaaatgga
     2761 agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga accaagatcc aaactcaaaa
     2821 agggcaaatt ccaaggagaa ttacatcaag tgccaagctg gcctaacttc agtctccacc
     2881 cactcagtgt ggggaaactc catcgcataa aacccctccc cccaacctaa agacgacgta
     2941 ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg cccggtactc cgtggagtca
     3001 catgaagcga cggctgagga cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc
     3061 cgctctcccg agcgccgcgt cctccatttt gagctccctg cagcagggcc gggaagcggc
     3121 catctttccg ctcacgcaac tggtgccgac cgggccagcc ttgccgccca gggcggggcg
     3181 atacacggcg gcgcgaggcc aggcaccaga gcaggccggc cagcttgaga ctacccccgt
     3241 ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca
     3301 cgggccccgt cgccgcccgc ggccccaaaa accgaaatac cagtgtgcag atcttggccc
     3361 gcatttacaa gactatcttg ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa
     3421 atggctagag acttatcgaa agcagcgaga caggcgcgaa ggtgccacca gattcgcacg
     3481 cggcggcccc agcgcccagg ccaggcctca actcaagcac gaggcgaagg ggctccttaa
     3541 gcgcaaggcc tcgaactctc ccacccactt ccaacccgaa gctcgggatc aagaatcacg
     3601 tactgcagcc aggtggaagt aattcaaggc acgcaagggc cataacccgt aaagaggcca
     3661 ggcccgcggg aaccacacac ggcacttacc tgtgttctgg cggcaaaccc gttgcgaaaa
     3721 agaacgttca cggcgactac tgcacttata tacggttctc ccccaccctc gggaaaaagg
     3781 cggagccagt acacgacatc actttcccag tttaccccgc gccaccttct ctaggcaccg
     3841 gttcaattgc cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc
     3901 cactgacggg caccggagcc tcacgactag tggatctatc gatttacttg tacacaggga
     3961 cagcagagat ccagtttgga ctagtggcct ccgcgccggg ttttggcgcc tcccgcgggc
     4021 gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat
     4081 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
     4141 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
     4201 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
     4261 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
     4321 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
     4381 ttggcggccc tagaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     4441 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     4501 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     4561 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     4621 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     4681 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     4741 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     4801 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     4861 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     4921 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4981 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     5041 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     5101 agctgtacaa gtaaagcggc cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa
     5161 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5221 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5281 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5341 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5401 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5461 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5521 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5581 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5641 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5701 tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa
     5761 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5821 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5881 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5941 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6001 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     6061 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     6121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6241 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6301 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6361 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     6421 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     6481 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6541 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6601 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6661 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6721 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6781 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6841 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6901 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6961 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7021 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     7081 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7141 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7201 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7261 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7321 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7381 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7441 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7501 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7561 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7621 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7681 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7741 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7801 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7861 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7921 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7981 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8041 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8101 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8161 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8221 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8281 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8341 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8401 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8461 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8521 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8581 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8641 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8701 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8761 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8821 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8881 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8941 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     9001 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     9061 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     9121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     9181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     9241 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     9301 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     9361 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     9421 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     9481 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     9541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9661 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     9721 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     9781 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     9841 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     9901 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     9961 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
    10021 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
    10081 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
    10141 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
    10201 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
    10261 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    10321 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    10381 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    10441 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    10501 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    10561 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    10621 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    10681 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    10741 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    10801 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    10861 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    10921 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    10981 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    11041 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
    11101 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
    11161 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact
    11221 agcctcgaga agcttgatat catctccata agagaagagg gacagctatg actgggagta
    11281 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
    11341 aagaaaaaaa taacacaaaa caaaatat
//
