LOCUS       sequence-438353-        3023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  601 nucleosome positioning sequence with an additional base pair 
            inserted 22 nt from the dyad on the TA-rich side of the 601.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Nodelman IM, Das S, Faustino AM, Fried SD, Bowman GD, Armache JP
  TITLE     Nucleosome recognition and DNA distortion by the Chd1 remodeler in a
            nucleotide-free state.
  JOURNAL   Nat Struct Mol Biol. 2022 Feb;29(2):121-129. doi: 
            10.1038/s41594-021-00719-x. Epub 2022 Feb 16.
  PUBMED    35173352
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-021-00719-x"; journalName: "Nat Struct Mol Biol"; 
            date: "2022-02"; volume: "29"; issue: "2"; pages: "121-129"
COMMENT     Sequence Label: pGEM-601[insert(+1) TA-rich]
FEATURES             Location/Qualifiers
     source          1..3023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(350..368)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(351..368)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(386..402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(386..402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(399..421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    410..426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(434..440,441..458,459..464))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    479..500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(617..634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(788..1375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(868..887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1546..2337,2338..2406))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2169..2188
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2407..2511)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2634..2656)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2756..2775
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2969..2991
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2983..3000
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2984..3000
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3007..3023,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3007..3023,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattc gagctcggta cccggggatc ctctagagtg ggagctcgga acactatccg
       61 actggcaccg gcaaggtcgc tgttcaatac atgcacagga tgtatatatc tgacacgtgc
      121 ctggagacta gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg
      181 tttaagcggt tgctagagct gtctacgacc aattgagcgg cctcggcacc gggattctcc
      241 agggcggccg cgtatagggt ccatcacata agggatgaac tcggtgtgaa gaatcatgct
      301 ttccttggtc attaggatcc cggacctgca ggcatgcaag cttgagtatt ctatagtgtc
      361 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      421 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      481 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      541 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      601 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      661 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      721 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      781 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      841 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      901 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      961 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1021 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1081 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1141 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1201 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1261 cagttacctt cggaaaaaga gttggtagct cttgatcggc aaacaaacca ccgctggtag
     1321 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1381 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1441 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1501 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1561 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1621 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1681 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1741 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1801 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1861 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1921 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1981 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2041 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2101 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2161 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2221 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2281 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2341 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2401 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2461 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2521 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     2581 tagcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     2641 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     2701 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     2761 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     2821 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     2881 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     2941 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     3001 gaattgtaat acgactcact ata
//
