LOCUS       sequence-438468-        9058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and 
            emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF 
            expression is characterized in publication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Duan M, Dev I, Lu A, Ayrapetyan G, You MY, Shapiro MG
  TITLE     SEMPER: Stoichiometric expression of mRNA polycistrons by eukaryotic
            ribosomes for compact, ratio-tunable multi-gene expression.
  JOURNAL   Cell Syst. 2024 Jul 17;15(7):597-609.e4. doi: 
            10.1016/j.cels.2024.06.001. Epub 2024 Jul 5.
  PUBMED    38971149
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2024.06.001"; journalName: "Cell Syst"; date: 
            "2024-07-17- 17"; volume: "15"; issue: "7"; pages: "597-609.e4"
COMMENT     Sequence Label: 
            pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCher
            ry
FEATURES             Location/Qualifiers
     source          1..9058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     758..775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(849..953)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1021..1039
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(1066..1071,1072..1089,1090..1096)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1104..1120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1109..1131
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     enhancer        1150..1453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1454..1657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1608..1628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1654..1678
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1773..1791
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1773..1790
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3304..3313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4284..4835
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4428..4445)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4655..4674
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4847..5557
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4992..5010)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5260..5279
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5611..5630)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5612..5630)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6025..6613
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6078..6098)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6496..6507)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6633..7109
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6832..6851)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      7245..7699
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7332..7351)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7541..7562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             join(7986..8054,8055..8846)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAVLGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8204..8223)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    8948..8981
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttattcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      781 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      841 agcttgcaat tcgcgctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      901 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      961 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     1021 aataggcgta tcacgaggcc ctttcactca ttaggcaccc caggctttac actttatgct
     1081 tccggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcat
     1141 cgtgcaggtc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     1201 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     1261 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     1321 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     1381 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     1441 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     1501 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     1561 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     1621 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     1681 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
     1741 ccggactcta gcctaggctt ttgcaaaaag ctatttaggt gacactatag aaggtacgcc
     1801 tgcaggtacc gagctcggat ccagtagttt aaaccccttc accatggtta gtaaaggaga
     1861 agaactcttc accggcgtag ttccgattct cgttgagctg gacggagacg ttaacgggca
     1921 caaattctct gtaaggggtg aaggggaagg cgacgctacc aacggaaaac tgaccttaaa
     1981 atttatttgt acaacaggta agctcccagt tccctggccc acccttgtta ccactctgac
     2041 tcacggcgtt cagtgtttct ctcgttatcc agatcatata aagcggcacg acttctttaa
     2101 gtccgctctc cccgaaggct acgtccaaga aagaaccatt agttttaagg acgacggaac
     2161 ttacaaaacc agagcagagg tcaagttcga gggagatact ctcgtcaatc ggatcgaact
     2221 gaaaggaata gactttaaag aggacggcaa tatcctgggt cacaagctgg agtataattt
     2281 caatagccat aacgtgtata tcacggccga taagcagaag aacggtatta aggcaaattt
     2341 taagattcgc cataacgtag aagacggttc cgtccaattg gcggaccatt atcaacaaaa
     2401 cactccaatt ggagacggcc cggtattact gcctgataat cactacctgt ccacccaatc
     2461 aaaactctcc aaagatccca acgagaaacg ggaccacgcg gttctgttag aatttgtaac
     2521 ggccgctggt attacgcacg gtaaggacga actgtataag tgaagcgctt taccatggtg
     2581 tccaagggcg aggaactgtt caccggcgtg gtgcccatcc tggtggaact ggacggcgac
     2641 gtgaacggcc acaagttcag cgtgagaggc gagggcgaag gcgacgccac aaacggaaag
     2701 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccttggcc taccctcgtg
     2761 accacactga cctacggcgt gcagtgcttc agcagatacc ccgaccacat caagagacac
     2821 gatttcttca agagcgccct gcccgagggc tacgtgcagg aacggaccat cagcttcaag
     2881 gacgacggca cctacaagac aagagccgaa gtgaagttcg agggcgacac cctcgtgaac
     2941 cggatcgagc tgaagggcat cgacttcaaa gaggacggca acatcctggg ccacaagctg
     3001 gagtacaact tcaacagcca caacgtgtac atcaccgccg acaagcagaa aaacggcatc
     3061 aaagccaact tcaagatccg gcacaacgtg gaggacggca gcgtgcagct ggccgaccac
     3121 taccagcaga acacccccat cggagacggc cccgtgctgc tgcccgacaa ccactacctg
     3181 agcacacaaa gcaagctgag caaggacccc aacgagaagc gggaccacgc cgtgctgctg
     3241 gaatttgtga ccgccgctgg catcacccac ggcaaggacg agctgtacaa gtgacccggg
     3301 ttaccatggt ggcggaaggc tccgtcgcca ggcagcctga cctcttgacc tgcgaacatg
     3361 aagagatcca cctcgccggc tcgatccagc cgcatggcgc gcttctggtc gtcagcgaac
     3421 atgatcatcg cgtcatccag gccagcgcca acgccgcgga atttctgaat ctcggaagcg
     3481 tactcggcgt tccgctcgcc gagatcgacg gcgatctgtt gatcaagatc ctgccgcatc
     3541 tcgatcccac cgccgaaggc atgccggtcg cggtgcgctg ccggatcggc aatccctcta
     3601 cggagtactg cggtctgatg catcggcctc cggaaggcgg gctgatcatc gaactcgaac
     3661 gtgccggccc gtcgatcgat ctgtcaggca cgctggcgcc ggcgctggag cggatccgca
     3721 cggcgggttc actgcgcgcg ctgtgcgatg acaccgtgct gctgtttcag cagtgcaccg
     3781 gctacgaccg ggtgatggtg tatcgtttcg atgagcaagg ccacggcctg gtattctccg
     3841 agtgccatgt gcctgggctc gaatcctatt tcggcaaccg ctatccgtcg tcgactgtcc
     3901 cgcagatggc gcggcagctg tacgtgcggc agcgcgtccg cgtgctggtc gacgtcacct
     3961 atcagccggt gccgctggag ccgcggctgt cgccgctgac cgggcgcgat ctcgacatgt
     4021 cgggctgctt cctgcgctcg atgtcgccgt gccatctgca gttcctgaag gacatgggcg
     4081 tgcgcgccac cctggcggtg tcgctggtgg tcggcggcaa gctgtggggc ctggttgtct
     4141 gtcaccatta tctgccgcgc ttcatccgtt tcgagctgcg ggcgatctgc aaacggctcg
     4201 ccgaaaggat cgcgacgcgg atcaccgcgc ttgagagctg ataatctaga tacactaaat
     4261 cgcactgtcg gcgtcccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     4321 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     4381 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     4441 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     4501 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     4561 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     4621 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     4681 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     4741 cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg
     4801 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg tgagcaaggg
     4861 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     4921 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     4981 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     5041 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     5101 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     5161 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     5221 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     5281 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     5341 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     5401 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     5461 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     5521 ccactccacc ggcggcatgg atgagctgta caagtgaagg atccctcgag gggcccaagc
     5581 ttacgcgtgc atgcgacgtc atagctctct ccctatagtg agtcgtatta taagctagct
     5641 tgggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     5701 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactagc
     5761 tgcatatgct tgctgcttga gagttttgct tactgagtat gatttatgaa aatattatac
     5821 acaggagcta gtgattctaa ttgtttgtgt attttagatt cacagtccca aggctcattt
     5881 caggcccctc agtcctcaca gtctgttcat gatcataatc agccatacca catttgtaga
     5941 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     6001 tgcaattgtt gttgttcgtg gctgaatcaa cctctggatt acaaaatttg tgaaagattg
     6061 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6121 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6181 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6241 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6301 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6361 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6421 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6481 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6541 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6601 gccgcctccc cgccagagac aattaacttc gcgggtggca tccctgtgac ccctccccag
     6661 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
     6721 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
     6781 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
     6841 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
     6901 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
     6961 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
     7021 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
     7081 cgtgaaccac tgctcccttc cctgtccttg aagttctcag atcctgcatt aatgaatcgg
     7141 ccaacgcgcg gggagaggcg gtttgcgtat tggctggcgt aatagcgaag aggcccgcac
     7201 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     7261 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7321 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     7381 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     7441 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     7501 ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt gttccaaact
     7561 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7621 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7681 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7741 gtttattttt ctaaatacat tcaaatatgt atccgctcat gccaggtctt ggactggtga
     7801 gaacggcttg ctcggcagct tcgatgtgtg ctggagggag aataaaggtc taagatgtgc
     7861 gatagaggga agtcgcattg aattatgtgc tgtgtaggga tcgctggtat caaatatgtg
     7921 tgcccacccc tggcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7981 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8041 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8101 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8161 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8221 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8281 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8341 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8401 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8461 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8521 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     8581 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8641 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8701 ggtctcgcgg tatcattgca gtactggggc cagatggtaa gccctcccgt atcgtagtta
     8761 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8821 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8881 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8941 tcatgccata acttcgtata atgtatgcta tacgaagtta tggcatgacc aaaatccctt
     9001 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
