LOCUS       sequence-438544-        8353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TBK1 protein overexpression in Sf9 insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Landau LM, Chaudhary N, Tien YC, Rogozinska M, Joshi S, Yao C, 
            Crowley J, Hullahalli K, Campbell IW, Waldor MK, Haigis M, Kagan JC
  TITLE     pLxIS-containing domains are biochemically flexible regulators of 
            interferons and metabolism.
  JOURNAL   Mol Cell. 2024 Jul 11;84(13):2436-2454.e10. doi: 
            10.1016/j.molcel.2024.05.030. Epub 2024 Jun 25.
  PUBMED    38925114
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05.030"; journalName: "Mol Cell"; date: 
            "2024-07-11- 11"; volume: "84"; issue: "13"; pages: "2436-2454.e10"
COMMENT     Sequence Label: pFastBac-GST-TBK1
FEATURES             Location/Qualifiers
     source          1..8353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..666
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             673..1326
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             1345..1365
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3724..3858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3761..3780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3815..3834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3887..4052
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4236..4690
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4323..4342)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4532..4553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4716..4820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4821..4889,4890..5681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5039..5058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5852..6440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6341..6360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6605..6622
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6756..6980
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(7047..7580)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7750..7755,7756..7772,7773..7778))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        8130..8221
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     8146..8163
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             8288..8305
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8327..8347
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggatccgcca ccatgagtcc gattttgggt tactggaaaa ttaaaggctt ggtccagccg
       61 actaggctgt tgctggaata tctggaagaa aaatacgaag aacatcttta cgagagagac
      121 gagggagata aatggagaaa taagaaattt gagctgggtt tggagtttcc taacctcccg
      181 tactatattg atggagacgt caagcttacg cagtccatgg ctattatacg ctacattgcc
      241 gataagcaca acatgttggg tggttgccca aaagagcgtg cagagattag catgctggag
      301 ggcgcggtcc ttgacatccg ttatggtgta tcacgtatag cgtactcaaa ggactttgag
      361 acgctgaaag tcgacttcct tagcaaactt cccgaaatgc tgaagatgtt cgaggaccgt
      421 ctctgccata aaacctatct caacggcgat cacgtgacgc accccgactt catgctttac
      481 gacgcgctgg acgtcgttct gtatatggac cccatgtgcc tcgatgcatt ccctaagctc
      541 gtatgtttta agaaacgcat agaagcaata ccacagattg acaagtacct gaaaagctct
      601 aaatatattg cttggccgct gcaaggttgg caggcgacgt ttggtggcgg tgatcatccg
      661 ccgaagtcgg atatgagccc aatactcggt tactggaaaa taaaaggtct ggttcagccg
      721 actcgtctcc ttttggaata ccttgaagaa aagtatgagg agcatctgta tgaacgtgac
      781 gagggtgaca agtggagaaa taagaaattc gagctcggct tggaatttcc taacctccct
      841 tattatatcg atggagatgt gaaactcaca cagtcaatgg ctattatcag gtacatcgcg
      901 gataagcata atatgcttgg aggctgtccg aaggaacgcg ctgagatttc gatgctggaa
      961 ggcgctgttc tcgatattag gtacggtgtc agtagaatcg cctactcaaa agacttcgaa
     1021 acgttgaagg tcgattttct cagcaagctg cccgagatgc tcaagatgtt tgaagatagg
     1081 ctgtgccaca agacctactt gaacggtgac catgtcaccc acccggattt catgttgtac
     1141 gacgcactcg acgtagtcct ctatatggac cctatgtgtt tggatgcctt cccaaagctg
     1201 gtgtgcttca aaaagaggat cgaggccata ccacagatcg ataaatatct taagtcttcg
     1261 aagtatatcg catggccact tcaaggttgg caggccactt ttggtggagg tgatcatccg
     1321 cctaagggtg ctggagcggg tgctgagaac ctctacttcc agggtggtgc tggcgctgga
     1381 gctatgcaat cgacttccaa tcatttgtgg ctgctgtcag atattcttgg ccagggagca
     1441 acagcaaatg tattccgtgg taggcacaaa aaaacaggag atttgtatgc tgtcaaagta
     1501 tttaacaaca tttccttctt gagaccagtg gatgtacaaa tgcgcgagtt cgaagttctc
     1561 aaaaaactga atcataagaa catagtaaaa cttttcgcaa tagaagagga gaccacgacg
     1621 agacataagg tccttataat ggagttctgc ccatgcggaa gcctgtacac tgtcttggaa
     1681 gagccaagta atgcctatgg cctgccggag agcgaattcc ttatagtgtt gagagatgtg
     1741 gttggaggca tgaaccatct ccgcgaaaat ggcatagtcc acagagatat aaaacctggc
     1801 aacataatga gggtaatagg tgaagatgga cagagtgtct ataagctcac cgactttgga
     1861 gctgctcgcg agttggagga tgacgagcaa ttcgtgtctt tgtatggaac tgaagaatac
     1921 ttgcatccag atatgtatga gagggctgtg cttaggaaag atcaccagaa aaagtacggc
     1981 gctacggtcg atctctggtc tgtaggagtg acgttctacc acgctgccac tggttccctt
     2041 ccttttaggc cttttgaagg tccgcgccgc aacaaggagg tgatgtataa gataattacg
     2101 ggcaagcctt cgggtgcaat ttcaggtgta caaaaggctg agaatggccc tatcgactgg
     2161 tccggcgata tgccgttgtc gtgctccttg tcgcagggac tgcaggcatt gcttacccct
     2221 gtgttggcaa acattctgga ggctgatcaa gagaagtgtt ggggatttga tcagttcttt
     2281 gcggagacta gtgacgtctt gcataggatg gtaatccacg tattttctct tcagcatatg
     2341 actgcacata aaatctatat acatagctac aataccgcgg ccgtctttca cgaactcgtg
     2401 tataagcaga caaaaattgt gagtagcaac caagaattga tctacgaggg caggagactc
     2461 gttttggaac tcggtaggct ggcacaacat ttccctaaaa cgactgagga gaaccctata
     2521 ttcgtaacaa gtcgcgaaca gcttaacacc gtcggcctca ggtacgaaaa aatatctttg
     2581 cccaaaatcc atcccaggta tgatcttgat ggagatgcga gtatggcgaa ggcggtaact
     2641 ggcgtcgtgt gctacgcgtg tcgtacagcc tcaactttgc tcctctatca agaactcatg
     2701 cgtaaaggag tccgctggtt ggtcgaattg gttaaagacg actacaacga gacggtccac
     2761 aagaaaaccg aggtcgtcat aacactcgac ttctgcattc gcaacattga gaaaacggtt
     2821 aaggtctacg agaaacttat gaaagtaaac ttggaagccg ctgagctcgg cgaaatctcc
     2881 gatatacaca caaagctcct gagacttagt agttcacaag gaaccataga gtcctcactc
     2941 caagacattt cctcacgcct ttctccggga ggacttttgg cggatacttg ggcgcatcag
     3001 gaaggaacac acccacgtga tagaaatgtt gaaaagcttc aagtgctcct caattgcatc
     3061 actgaaatat attatcaatt taagaaggat aaagcagagc gtagactggc atacaatgag
     3121 gagcagattc acaagttcga taagcaaaag ctttactatc atgcgaccaa agcgatgtca
     3181 cacttttcag aggagtgcgt gagaaaatat gaagctttca aagataagag cgaagaatgg
     3241 atgaggaaga tgttgcacct taggaagcag ctccttagtc ttaccaacca atgcttcgat
     3301 attgaggagg aagtcagtaa gtaccaagat tatactaatg agttgcaaga gacattgccg
     3361 cagaagatgc tcgcagcgtc tggcggcgtc aaacacgcta tggcaccaat atatccctct
     3421 agcaataccc tcgtcgaaat gactctggga atgaaaaaat tgaaagagga aatggagggc
     3481 gtcgttaaag agctggcgga gaacaatcac atacttgaga gatttggatc gcttaccatg
     3541 gacggtggtt tgcgcaatgt cgattgcctc tgataactcg aggcatgcgg taccaagctt
     3601 gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct
     3661 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     3721 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3781 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3841 tcttatcatg tctggatctg atcactgata tcgcctagga gatccgaacc agataagtga
     3901 aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc
     3961 cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc
     4021 ctcccagttc ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt
     4081 ttaatcaaac atcctgccaa ctccatgtga caaaccgtca tcttcggcta ctttttctct
     4141 gtcacagaat gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat
     4201 atcaacgctt atttgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     4261 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4321 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4381 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4441 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4501 ccctttgacg ttggagtcca cgttcttaat agtggactct tgttccaaac tggaacaaca
     4561 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4621 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4681 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6481 gctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6541 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6601 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     6661 gcatctgtgc ggtatttcac accgcataga ccagccgcgt aacctggcaa aatcggttac
     6721 ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa
     6781 ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa
     6841 actgaaatca gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc
     6901 aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg
     6961 gcatggtaaa gactatattc gcggcgttgt gacaatttac cgaacaactc cgcggccggg
     7021 aagccgatct cggcttgaac gaattgttag gtggcggtac ttgggtcgat atcaaagtgc
     7081 atcacttctt cccgtatgcc caactttgta tagagagcca ctgcgggatc gtcaccgtaa
     7141 tctgcttgca cgtagatcac ataagcacca agcgcgttgg cctcatgctt gaggagattg
     7201 atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag atcatagata
     7261 tagatctcac tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca
     7321 accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct tactacggag caagttcccg
     7381 aggtaatcgg agtccggctg atgttgggag taggtggcta cgtctccgaa ctcacgaccg
     7441 aaaagatcaa gagcagcccg catggatttg acttggtcag ggccgagcct acatgtgcga
     7501 atgatgccca tacttgagcc acctaacttt gttttagggc gactgccctg ctgcgtaaca
     7561 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
     7621 gcccgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     7681 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     7741 ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc cttcatccgt
     7801 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
     7861 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
     7921 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
     7981 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
     8041 ggttttctgg aaggcgagca tcgtttgttc gcccaggact ctagctatag ttctagtggt
     8101 tggctacgta tactccggaa tattaataga tcatggagat aattaaaatg ataaccatct
     8161 cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa aacctataaa
     8221 tattccggat tattcatacc gtcccaccat cgggcgcgga tctcggtccg aaaccatgtc
     8281 gtactaccat caccatcacc atcacgatta cgatatccca acgaccgaaa acctgtattt
     8341 tcagggcgcc atg
//
