LOCUS       sequence-438564-       14661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14661)
  AUTHORS   Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R
  TITLE     Cas9 effector-mediated regulation of transcription and 
            differentiation in human pluripotent stem cells.
  JOURNAL   Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
  PUBMED    24346702
REFERENCE   2  (bases 1 to 14661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-01"; volume: "141"; issue: 
            "1"; pages: "219-23"
COMMENT     Sequence Label: pHAGE TRE dCas9-KRAB
FEATURES             Location/Qualifiers
     source          1..14661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             67..93
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        179..1390
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1227..1246
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(1449..2066,2067..2072,2073..2108,2109..2111,2112..2147,
                     2148..2150,2151..2186,2187..2195)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    2248..2821
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2415..2432)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2642..2661
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2837..3631
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2837..3631
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2891..2910)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3501..3520
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3808..4396
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3861..3881)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4279..4290)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4471..4704
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4823..4841)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4909..5013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5014..5082,5083..5874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5232..5251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6045..6633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6534..6553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6787..6804
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7044..7065
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7080..7085,7086..7103,7104..7110)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7118..7134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7123..7145
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7142..7158
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7142..7158
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7208..7227
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7419..8052
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8099..8224
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8721..8954
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9139..9183
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9476..9593
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(9648..9666,9667..9689,9690..9708,9709..9731,9732..9750,
                     9751..9773,9774..9792,9793..9815,9816..9834,9835..9857,
                     9858..9876,9877..9899,9900..9918)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9951..9989
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9986..10010
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    10119..10143
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      10147..10156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10153..10179,10180..10182,10183..12666,12667..12672,
                     12673..12738,12739..12741,12742..14256)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(10153..10179,10180..10182,10183..12669,12670..12672,
                     12673..14256)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10153..14256
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             14269..14289
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14290..14379
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             14416..14610
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     protein_bind    complement(join(14651..14661,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg
       61 ttcgaatacc catacgacgt cccagactac gcttagtaat gattaattaa actagaaatt
      121 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gtaacccacc caagatctgg
      181 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc
      241 acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg
      301 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
      361 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
      421 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
      481 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
      541 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
      601 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
      661 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
      721 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
      781 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg
      841 ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga
      901 cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta
      961 tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt
     1021 gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg
     1081 gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg
     1141 acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt
     1201 tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt
     1261 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
     1321 atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt
     1381 tttgttagac gaagcttggt accgagctcg gatctccacc ccgtaccggt cctgcagtcg
     1441 aattcaccat gtctagactg gacaagagca aagtcataaa cggagctctg gaattactca
     1501 atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc
     1561 agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg
     1621 agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact
     1681 ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta
     1741 aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg
     1801 cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg
     1861 gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa
     1921 gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg
     1981 accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg
     2041 agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag
     2101 acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc
     2161 ttgacgattt tgaccttgac atgctccccg ggtaactaag taaggatccg cggccgcact
     2221 agaggaattc cgcccctctc cccccccccc ctctccctcc ccccccccta acgttactgg
     2281 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     2341 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     2401 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     2461 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     2521 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     2581 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     2641 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     2701 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     2761 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     2821 agcttaccgg tccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     2881 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     2941 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     3001 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     3061 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     3121 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     3181 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     3241 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     3301 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     3361 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     3421 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     3481 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     3541 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     3601 cttctatcgc cttcttgacg agttcttctg atgtacaagt aaagcggccg cgactctaga
     3661 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     3721 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgtttagtcc ctcccaattc
     3781 gatatcaagc ttatcgcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     3841 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3901 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3961 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4021 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4081 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4141 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4201 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4261 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4321 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     4381 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     4441 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4501 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4561 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4621 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4681 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     4741 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     4801 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4861 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5221 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     5281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5521 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5701 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5881 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6181 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6721 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6781 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6841 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt
     6901 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6961 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa
     7021 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7081 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7141 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat
     7201 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
     7261 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
     7321 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
     7381 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
     7441 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     7501 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     7561 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     7621 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     7681 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     7741 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     7801 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     7861 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7921 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7981 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8041 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     8101 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     8161 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     8221 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     8281 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     8341 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     8401 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     8461 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     8521 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
     8581 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     8641 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     8701 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     8761 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     8821 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     8881 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     8941 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     9001 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     9061 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     9121 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     9181 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     9241 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     9301 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     9361 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     9421 gtgaacggat ctcgacggta tcgccgaatt cacaaatggc agtattcatc cacaatttta
     9481 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9541 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9601 attacaggga cagcagagat ccagtttgga ctaggatcct ttaccactcc ctatcagtga
     9661 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     9721 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     9781 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     9841 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     9901 ccctatcagt gatagagaaa agtgaaagtc gagctcggta cccgggtcga ggtaggcgtg
     9961 tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
    10021 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
    10081 ccgaactagt ccagtgtggt ggaattctgc agatatcaac aagtttgtac aaaaaagcag
    10141 gctggcgcca ccatggacaa gaagtactcc attgggctcg ctatcggtac caacagcgtc
    10201 ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc
    10261 aataccgatc gccacagcat aaagaagaac ctcattggag ccctcctgtt cgactccggg
    10321 gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag
    10381 aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct
    10441 ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac
    10501 ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat
    10561 catctgagga agaagctggt agacagtact gataaggctg acttgcggtt gatctatctc
    10621 gcgctggcgc acatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca
    10681 gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc
    10741 gaggagaacc cgatcaacgc atccggcgtt gacgccaaag caatcctgag cgctaggctg
    10801 tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc
    10861 ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc
    10921 gacctggccg aagatgccaa gctgcaactg agcaaagaca cctacgatga tgatctcgac
    10981 aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg
    11041 tcagacgcca ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg
    11101 ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag
    11161 gcccttgtca gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa
    11221 aatggctacg ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt
    11281 aagcccatct tggaaaaaat ggacggcacc gaggagctgc tggtaaagct gaacagagaa
    11341 gatctgttgc gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg
    11401 ggcgaactgc acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac
    11461 agggaaaaga ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgct
    11521 cggggaaatt ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg
    11581 aacttcgagg aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact
    11641 aactttgata aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag
    11701 tacttcacag tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag
    11761 ccagcattcc tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac
    11821 cggaaagtta ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac
    11881 tctgttgaaa tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat
    11941 ctcctgaaaa tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt
    12001 gaggacattg tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg
    12061 aaaacttacg ctcatctctt cgacgacaaa gtcatgaaac agctcaagag acgccgatat
    12121 acaggatggg ggcggctgtc aagaaaactg atcaatggca tccgagacaa gcagagtgga
    12181 aagacaatcc tggattttct taagtccgat ggatttgcca accggaactt catgcagttg
    12241 atccatgatg actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag
    12301 ggggacagtc ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga
    12361 atactgcaga ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc
    12421 gagaatatcg ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac
    12481 agtagggaaa ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt
    12541 aaggaacacc cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg
    12601 cagaacggca gggacatgta cgtggatcag gaactggaca tcaaccggtt gtccgactac
    12661 gacgtggatg ctatcgtgcc ccaaagcttt ctcaaagatg attctattga taataaagtg
    12721 ttgacaagat ccgataaaaa tagagggaag agtgataacg tcccctcaga agaagttgtc
    12781 aagaaaatga aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag
    12841 ttcgataatc tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc
    12901 atcaaaaggc agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat
    12961 tcacgcatga acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt
    13021 actctgaagt ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga
    13081 gagatcaaca attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca
    13141 cttatcaaaa aatatcccaa gctggaatct gaatttgttt acggagacta taaagtgtac
    13201 gatgttagga aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac
    13261 ttcttttaca gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag
    13321 attcggaagc gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag
    13381 ggtagggatt tcgcgacagt ccgcaaggtc ctgtccatgc cgcaggtgaa catcgttaaa
    13441 aagaccgaag tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc
    13501 gacaagctga tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct
    13561 cctacagtcg cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa
    13621 ctcaaaagcg tcaaggaact gctgggcatc acaatcatgg agcgatccag cttcgagaaa
    13681 aaccccatcg actttctcga agcgaaagga tataaagagg tcaaaaaaga cctcatcatt
    13741 aagctgccca agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt
    13801 gcgggcgagc tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg
    13861 tatctggcca gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag
    13921 ctgttcgtgg aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgagttc
    13981 tccaaaagag tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag
    14041 cacagggata agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc
    14101 aacttgggcg cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac
    14161 acctctacaa aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat
    14221 gaaacaagaa tcgacctctc tcagctcggt ggagacggca ccggcgggcc caagaagaag
    14281 aggaaggtat acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac
    14341 tatgcaggat cgtatcctta tgacgttcca gattacgctg gatccatgga cgcgaaatca
    14401 cttacggcat ggtcgagaac actggttacg ttcaaggacg tgtttgtgga ctttacacgt
    14461 gaggagtgga aattgctgga tactgcgcaa caaattgtgt atcgaaatgt catgcttgag
    14521 aattacaaga acctcgtcag tctcggatac cagttgacga aaccggatgt gatccttagg
    14581 ctcgaaaagg gggaagaacc ttggctggta taattcgaat ccggataagc ggccgcctcg
    14641 agatatctag acccagcttt c
//
