LOCUS       sequence-438710-       14496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional reporter for ARF7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14496)
  TITLE     Bishopp Lab ARF7 Reporter Plasmids 2024
REFERENCE   2  (bases 1 to 14496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pro::GFP
FEATURES             Location/Qualifiers
     source          1..14496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    83..107
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(198..786)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(278..297)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(957..1745)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2036..2471
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2598..2620
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        2670..2688
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2670..2687
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(2721..3428)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    3447..3471
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5274..5329
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    complement(5351..5375)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(10391..10393,10394..10396,10397..11107)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(10436..10457)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10697..10716)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11044..11065
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      11416..12057
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             12740..13768
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      13776..14483
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 ggtacctggt gcgatcgctg ttggcgcgcc gtgtttaatt aaggttgcgg ccgcttactt
       61 cgtccgagcc tagttcgagc cttgacagga tatattggcg ggtaaactaa gtcgctgtat
      121 gtgtttgttt gagatctcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      181 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      241 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      301 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      361 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      421 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      481 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      541 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      601 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      661 ctgctgaagc cagttacctt cggaagaaga gttggtagct cttgatccgg caaacaaacc
      721 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      781 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      841 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      901 taaaaatgaa gttttaaatc aatctaaagt atatatgtgt aacattggtc tagtgattat
      961 ttgccgacta ccttggtgat ctcgcctttc acgtagtgaa caaattcttc caactgatct
     1021 gcgcgcgagg ccaagcgatc ttcttgtcca agataagcct gcctagcttc aagtatgacg
     1081 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
     1141 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
     1201 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
     1261 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
     1321 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
     1381 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
     1441 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
     1501 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
     1561 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
     1621 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
     1681 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga
     1861 ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac
     1921 cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc
     1981 atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc gatccccatc
     2041 caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata gagggtagtt
     2101 atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt ccggcccgct
     2161 ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa tcggagtacg
     2221 tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga gaacgctaat
     2281 agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt tacagcaagt
     2341 agtatgttca attagctttt caattatgaa tatatatatc aattattggt cgcccttggc
     2401 ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag cggttgccca
     2461 ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt ttataaattt
     2521 ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct ggatttccga
     2581 tccccggaat tagatcttgg caggatatat tgtggtgtaa cgttggatct ggctgagaac
     2641 gccagctgtg catgcttggt ctagaataca tttaggtgac actatagaat atcactagta
     2701 agctagcttg catgccggtc ctgctgagcc tcgacatgtt gtcgcaaaat tcgccctgga
     2761 cccgcccaac gatttgtcgt cactgtcaag gtttgacctg cacttcattt ggggcccaca
     2821 tacaccaaaa aaatgctgca taattctcgg ggcagcaagt cggttacccg gccgccgtgc
     2881 tggaccgggt tgaatggtgc ccgtaacttt cggtagagcg gacggccaat actcaacttc
     2941 aaggaatctc acccatgcgc gccggcgggg aaccggagtt cccttcagtg agcgttatta
     3001 gttcgccgct cggtgtgtcg tagatactag cccctggggc acttttgaaa tttgaataag
     3061 atttatgtaa tcagtctttt aggtttgacc ggttctgccg ctttttttaa aattggattt
     3121 gtaataataa aacgcaattg tttgttattg tggcgctcta tcatagatgt cgctataaac
     3181 ctattcagca caatatattg ttttcatttt aatattgtac atataagtag tagggtacaa
     3241 tcagtaaatt gaacggagaa tattattcat aaaaatacga tagtaacggg tgatatattc
     3301 attagaatga accgaaaccg gcggtaagga tctgagctac acatgctcag gttttttaca
     3361 acgtgcacaa cagaattgaa agcaaatatc atgcgatcat aggcgtctcg catatctcat
     3421 taaagcagga ctctagagga tcatcaacca ctttgtacaa gaaagctggg tcggcgcgcc
     3481 caccctttta cttgtacaac tcgtccatac cgtagaggaa gaggtggtgg cggccctcgg
     3541 aacgctcgta ctgttcgaca atggtgtaat cctcgttgtg ggaggtaatg tccaacttgg
     3601 tatcaacgta gtaataacca ggcaattgaa cgggtttctt ggccatgtag atggtcttaa
     3661 actccacgag gtaatgtcca ccgtccttca acttcaatgc ctggtgaatc tcgcccttca
     3721 acactccatc gcggggatac aagcgctctg ttgaagcctc ccagcccata gtcttcttct
     3781 gcataactgg gccgtctggt gggaaattgg ttccacgcat tttgaccttg tagataagcg
     3841 taccatcttg aagggaggag tcttgggtga ctgtaaccaa accaccgtcc tcgaagttca
     3901 taacgcgctc ccacttaaat ccttctggga aagacaactt cttataatct ggaatatcgg
     3961 ctgggtgttt cacgtaagcc ttggaaccgt acataaactg tggtgagaga atatcccaag
     4021 cgaaaggaag tggtccaccc ttggtgactt tcaacttagc tgtctgagtg ccctcatatg
     4081 ggcggccctc accctctcct tcgatctcga actcgtggcc attcattgag ccctccatac
     4141 ggactttgaa gcacataaac tccttgatca cagccatgtt attatcctcg gatgaagcgg
     4201 tgccagagct gccactaccg gtgcttccag taccatgacc caagaaaagg tgatggcgac
     4261 cctcagacct ctcatattgt tcaacgatgg tgtagtcctc gttgtgggag gtgatatcga
     4321 gtttagtgtc cacgtagtaa tagccgggga gttgcacggg cttcttagcc atgtagatgg
     4381 tcttgaactc caccaagtag tggcctccat ccttaagttt gagagcttgg tggatttcac
     4441 ccttaagaac gccgtctctt gggtaaagcc tctcagtgga ggcttcccaa cccatagttt
     4501 tcttttgcat gactggacca tcgggaggaa agtttgtgcc tctcatcttc accttgtaga
     4561 tcaaagtgcc gtcctgcaaa ctagaatctt gggtcacagt cacaagacct ccatcctcga
     4621 agttcatgac cctttcccac ttgaaaccct ctgggaagga caactttttg taatcgggga
     4681 tgtcagcagg atgcttaacg tatgccttag agccatacat gaattggggg gacaagatgt
     4741 cccaagcgaa gggcaaagga ccacccttag tcactttgag ctttgcggtt tgggtgccct
     4801 cgtagggtct accttcaccc tcgccctcga tctcgaactc gtgaccgttc atggaacctt
     4861 ccatcctaac cttgaatctc atgaactctt tgataacctc ctcacccttg gaaaccatgg
     4921 atcgggctgc cgcagcggca gcagccgcag caggtttggt gaggacataa atggcgtata
     4981 tgatcccagg aatataccca agtagcgtca aaaccaaaca tatccaaaac tcaacctgca
     5041 acaaaattca taatttcaac ttttaatttc tagtgtggat aaatgtaaaa aaagaaccat
     5101 atttgactaa agtataagat taattaacca aaataaaact cagtctaaat atgagaggaa
     5161 agataaatac cccgcaacca aatctgagaa agacaccgag tggaggcaag aggatggcga
     5221 taataatatc aacgaaagta gctgtactca ttttggtgaa gggggcggcc gctgtaattg
     5281 taaatagtaa ttgtaatgtt gtttgttgtt tgttgttgtt ggtaattgtt gtaaaaatac
     5341 gcggccgcgg agcctgcttt tttgtacaaa cttgttgatg atccctgtta atcagaaaaa
     5401 ctcagattaa tcgacaaatt cgatcgcaca aactagaaac taacaccaga tctagataga
     5461 aatcacaaat cgaagagtaa ttattcgaca aaactcaaat tatttgaaca aatcggatga
     5521 tatctatgaa accctaatcg agaattaaga tgatatctaa cgatcaaacc cagaaaatcg
     5581 tcttcgatct aagattaaca gaatctaaac caaagaacat atacgaaatt gggatcgaac
     5641 gaaaacaaaa tcgaagattt tgagagaata aggaacacag aaatttacct tgatcacggt
     5701 agagagaatt gagagaaagt ttttaagatt ttgagaaatt gaaatctgaa ttgtgaagaa
     5761 gaagagctct ttgggtattg ttttatagaa gaagaagaag aaaagacgag gacgactagg
     5821 tcacgagaaa gctaaggcgg tgaagcaata gctaataata aaatgacacg tgtattgagc
     5881 gttgtttaca cgcaaagttg tttttggcta attgccttat ttttaggttg aggaaaagta
     5941 tttgtgcttt gagttgataa acacgactcg tgtgtgccgg ctgcaaccac tttgacgccg
     6001 tttattactg actcgtcgac aaccacaatt tctaacggtc gtcataagat ccagccgttg
     6061 agatttaacg atcgttacga tttatatttt tttagcatta tcgttttatt ttttaaatat
     6121 acggtggagc tgaaaattgg caataattga accgtgggtc ccactgcatt gaagcgtatt
     6181 tcgtattttc tagaattctt cgtgctttat ttcttttcct ttttgttttt ttttgccatt
     6241 tatctaatgc aagtgggctt ataaaatcag tgaatttctt ggaaaagtaa cttctttatc
     6301 gtataacata ttgtgaaatt atccatttct tttaattttt tagtgttatt ggatattttt
     6361 gtatgattat tgatttgcat aggataatga cttttgtatc aagttggtga acaagtctcg
     6421 ttaaaaaagg caagtggttt ggtgactcga tttattcttg ttatttaatt catatatcaa
     6481 tggatcttat ttggggcctg gtccatattt aacactcgtg ttcagtccaa tgaccaataa
     6541 tattttttca ttaataacaa tgtaacaaga atgatacaca aaacattctt tgaataagtt
     6601 cgctatgaag aagggaactt atccggtcct agatcatcag ttcatacaaa cctccataga
     6661 gttcaacatc ttaaacaaga atatcctgat ctgaagaatg tggaggcttt agtcccttgg
     6721 atacttggga ggctgtggaa gaacagaaac gagctggtgc ttaaagggag ggaatttgga
     6781 accaatgagg tattagtaag gacacaagaa gatgcagatg agtggattag aaggaaagag
     6841 gctcagaatg taaggaaagc accaaccaca acaccgtcgg gacagacgag aaacatggga
     6901 agctccacca caatcttggg ttaagtgcaa ctttgatggg gcatggccaa cagaaggatt
     6961 aaaatgtggc ttagggtggg tgcttcgcga tcatacaggg aaggtgttat ggttaggtgc
     7021 aagagctgtg gtaaaagtaa gaagcgtgct ggaagtagaa gtggaggctc ttagatgggc
     7081 tgtgctgtca ttatcccgat tcaattatag gaagatcatt tttgaggtgg attctcagca
     7141 acttgtgtct ttggttacat gaaagttatg cttgtcaagt ctcaatccaa ttatccaaga
     7201 cataaagtat ctacttagca agtttgagga ttttatgctt gtgcatacaa gccgagaagg
     7261 aaatggagtg gcagatagaa tagctaagga atctctttct tttgagaatt atgatctaaa
     7321 gttgtattct attgtaccaa tttgggttaa aagctctgtt gagctagact catatgcata
     7381 cctaagagat gtcgcaaacc agctcatgtc accaaacaac aattagagcc aatgtgaaat
     7441 ggggtgggag tgtgaccacg tcaggtggga tttgccgtga gaggcccttc tttattgttt
     7501 gttccttttt cttcaattat tattttcaat aataatgtaa ttaaacttat catcaaatac
     7561 aaatattcga taaattaaag gatcctctac aagaaatatg aatattgata acacataaga
     7621 atagcaaaat ttttgtaact attattaaaa ataatattcg agatttgtta accatagtat
     7681 aacatttgta aaccactaaa cataaaagta acgagaaaat ctcctaaagc aaagtaacgt
     7741 ttctctatat ttagcgtttt acaaaattta tgttaaaaaa ttatgtttag cgttttacaa
     7801 aaattatgct agaaaagttt atgtttagcg gaaatctaaa atcacttaaa acgcaaaaaa
     7861 ttaatcagta cgtagattat tttccacaac tctctcttac atacaaatta acaaataatg
     7921 catatcagaa tgacatagta aagtcgagaa aatcatggaa tcgtaacgaa acttcctcgt
     7981 ctctctctga taattcatcc aactcaagtg gtgtattttg tggttacatt tacatatatt
     8041 gtcaacacca actgtcgaat tcattaaatc ggctaaaata cgttgatgtg attctggaaa
     8101 tatctttagg acagtatatg agttattcat cttgttcatt ttccactttc cactgcggac
     8161 accatacgga cacacaaccg gttctgaaat gcgtcacgct cttcttcctt tcattccaac
     8221 tttaatacat gcatgaagtc gtagttcgga gttcatacta aatttcggtc actttgtaat
     8281 aagaaaacta ttaacatttt tctttttctt tttttaaacg ttagctaggc ctcaaagttt
     8341 cacaaaaaaa aaagaaaaaa aaaagctagg cgtcgaaatc cataacttca aaaggccttc
     8401 ttacattcat ccaatctcca aacttctgtc ttcacgcatt tattgctcag ggacatcatg
     8461 tcgctactca ctgccactta tttgaattga catccattca ttccatacat atatattacc
     8521 ttcacttaaa gttgtaagta tatagaattt taagcattcc tgccaaataa gttttggatc
     8581 gaacctaaac taacaaagag gttactgttt tgtaactttc ttttttgatg tggggtcatt
     8641 caaagggttt acatgcattt tacaatggtc tgtgatgaat atggaggttc agtctttggt
     8701 agataaaact tgttatgtgg ccttaaacca aatagattgt agtttgtaca atgacatatt
     8761 tatggttata ttcggttttc caatatgttt aggaatatat atcaccgttt ttttttgggt
     8821 agacatttta acgattatag aggaaaattt tgaatgatgg ctgtactggg aaaagagtgt
     8881 aagttgtgat catcatatgt aattttattt tgtgtgacat tataacactt cttccaaaag
     8941 acaaaagttt aggttaatta atataagaaa ccaataaaga atcatgaaca aagagagaac
     9001 ttctatcatt tttgatatga aatcgtccgt taaaatattg ttctttgtga tcgcatatgc
     9061 tgtatgtact tagatttgat caattaatca acatttacaa caaaataaga tcaattgttt
     9121 ataatgatga tctaagcgaa tttagatttg tttgttatta tagaactcat tactaaacat
     9181 ttaagttcca ttctcgttta aaacatgaac gctagtttat gaaactcaga accaaccatg
     9241 taaaactcca caaaatttct agtaaaatgc ttttgttcaa aataatgaaa atcataaaat
     9301 tttttgatga attatgaatc ctaaaatcta tctgaattaa atatagttaa ctacaaaaga
     9361 aattgccaaa catatatgta cgcactttga tcaaataact agctagtcag cttagtttgt
     9421 ttttttcaat acatccccta taaatctatg ttatccctaa tcaagtatta caataaatga
     9481 tacagtaata ataaccttaa tgaaaaaaac ttatcaaatt atcagagata aggaaaaaga
     9541 aaacactaat aaagttgttt ttttgtgtgt caaagttaaa attttagagg acaataataa
     9601 cattaagaaa ttaaatcgtg gccagtttgc tagtgaatat gtcggcttca tgagcataac
     9661 taaataggta taaaaaaata aaaatatcgg aaaaagaaaa gagaaggaaa aaaaaaggag
     9721 aggaagaaga aaaagctgtt cctgtgcttt agcagacctt ttggtagttg tcctggtcgc
     9781 tgatcatccc tttccgacaa ctctgttgct ctttctctca cgtttgagtc ttaatcttcg
     9841 attgtccttg gtcatcctta agttttgttt ttctctttcg attcattttc cagatttctt
     9901 tttatagaaa cccgtctctt tgatttgatt ccccccaaga aaggaaagca aaacaaagaa
     9961 gagcagctga atcaagtaac tttgtgggtg tttttgaatt atctgactcg agataaagag
    10021 cttcgttaaa aacggaatct tttttaaggt cgtatgcttt gtttgtctct ccggagattc
    10081 cgcgtgtgaa aagtcgtggt ttttgatctc tttgagatac ttaaaatttc cgagaaattg
    10141 gcggctctgt cggagataaa aagctgagat cttggagttc cccatttctg attaacgatt
    10201 ctctctgctg ttacttgagc taggaagcta ttaacaactt gagtgaaagt ttagggcttt
    10261 tgatgaagct ctcacgcaaa gagcttagct ttgtataagc tcagattcag attatttatt
    10321 gggtttattc ttcagagaaa gtaaagttga gtgatcaaca gtattcagtc gactggtacc
    10381 aacaggctcc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
    10441 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
    10501 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
    10561 ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcagcc gctaccccga
    10621 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
    10681 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
    10741 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
    10801 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
    10861 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
    10921 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
    10981 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
    11041 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct
    11101 gtacaagatt gctgcagcgg ccgaattcaa gcgtgaagag caagcaagga aagctaaggt
    11161 gaacaatgag aaaaagacgg aaatagtgaa accagagagt tgtagcaatg aaggagatgt
    11221 caaggatctg aaaagaaagg actctgagga tggaaacgag ggtgaggaag aagaagcttc
    11281 ttcgaaaccg aaaaagccaa aagttgctct ttctcatctt caggacattg acgacacaga
    11341 agctgatcaa gaagaagagt caggtggatc ctagataacc tttaccttca ttccctgtaa
    11401 attggtaccc tgcaaagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca
    11461 atgcatcagt ttcattgcgc acacaccaga atcctactga gtttgagtat tatggcattg
    11521 ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg
    11581 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc
    11641 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat
    11701 ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc
    11761 ctctaatgac cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat
    11821 tcactaggca acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag
    11881 tatgtcctct tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct
    11941 tgtcagattc taatcattgc tttataatta tagttatact catggatttg tagttgagta
    12001 tgaaaatatt ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaatcg
    12061 actagtcgac gagtcagtaa taaacggcgt caaagtggtt gcagccggca cacacgagtc
    12121 gtgtttatca actcaaagca caaatacttt tcctcaacct aaaaataagg caattagcca
    12181 aaaacaactt tgcgtgtaaa caacgctcaa tacacgtgtc attttattat tagctattgc
    12241 ttcaccgcct tagctttctc gtgacctagt cgtcctcgtc ttttcttctt cttcttctat
    12301 aaaacaatac ccaaagagct cttcttcttc acaattcaga tttcaatttc tcaaaatctt
    12361 aaaaactttc tctcaattct ctctaccgtg atcaaggtaa atttctgtgt tccttattct
    12421 ctcaaaatct tcgattttgt tttcgttcga tcccaatttc gtatatgttc tttggtttag
    12481 attctgttaa tcttagatcg aagacgattt tctgggtttg atcgttagat atcatcttaa
    12541 ttctcgatta gggtttcata gatatcatcc gatttgttca aataatttga gttttgtcga
    12601 ataattactc ttcgatttgt gatttctatc tagatctggt gttagtttct agtttgtgcg
    12661 atcgaatttg tagattaatc tgagtttttc tgattaacac cacaccaatc tggtaccaga
    12721 aaaggggaga agcaaaacca tgggtaaaaa gcctgaactc accgcgacgt ctgtcgagaa
    12781 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
    12841 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
    12901 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
    12961 gattccggaa gtgcttgaca ttggggcatt cagcgagagc ctgacctatt gcatctcccg
    13021 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
    13081 accggtcgcg gaggcgatgg atgccatcgc tgcggccgat cttagccaga cgagcgggtt
    13141 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
    13201 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
    13261 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
    13321 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
    13381 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
    13441 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
    13501 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
    13561 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
    13621 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
    13681 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
    13741 cagcactcgt ccgagggcaa aggaatagat gcatcctgct ttaatgagat atgcgagacg
    13801 cctatgatcg catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca
    13861 tgtgtagctc agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta
    13921 ctatcgtatt tttatgaata atattctccg ttcaatttac tgattgtacc ctactactta
    13981 tatgtacaat attaaaatga aaacaatata ttgtgctgaa taggtttata gcgacatcta
    14041 tgatagagcg ccacaataac aaacaattgc gttttattat tacaaatcca attttaaaaa
    14101 aagcggcaga accggtcaaa cctaaaagac tgattacata aatcttattc aaatttcaaa
    14161 agtgccccag gggctagtat ctacgacaca ccgagcggcg aactaataac gctcactgaa
    14221 gggaactccg gttccccgcc ggcgcgcatg ggtgagattc cttgaagttg agtattggcc
    14281 gtccgctcta ccgaaagtta cgggcaccat tcaacccggt ccagcacggc ggccgggtaa
    14341 ccgacttgct gccccgagaa ttatgcagca tttttttggt gtatgtgggc cccaaatgaa
    14401 gtgcaggtca aaccttgaca gtgacgacaa atcgttgggc gggtccaggg cgaattttgc
    14461 gacaacatgt cgaggctcag caggaccggc atgcaa
//
