LOCUS       sequence-43895-e        9643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9643)
  TITLE     Lees Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lox-Stop-Lox TOPO Hygro
FEATURES             Location/Qualifiers
     source          1..9643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    362..395
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      426..435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    460..581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1006..1026)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1156..1221
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1314..1435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1860..1880)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          2010..2075
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    2168..2289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2714..2734)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          2864..2929
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    3022..3143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3568..3588)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          3718..3783
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        4393..4892
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4428..4451)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4825..4844
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4936..5961
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4936..5961
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    6452..6485
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6530..6549)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6531..6549)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6556..6573)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6556..6572)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6565..6587)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6710..7012
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6891..6910
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             7361..8155
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7361..8155
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7415..7434)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8025..8044
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      8356..8365
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8362..8736
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      8874..9462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9363..9382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9616..9633
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccaat ctcgagcctt
      301 aattaaccac gcgtggcgcg ccttgagctc ggatccgggc cccccctcga tcgaggtcga
      361 cataacttcg tataatgtat gctatacgaa gttattaggt ccctcgacct gcagacttac
      421 tcaagccatg gtggctagct tgcggaattc cagacatgat aagatacatt gatgagtttg
      481 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
      541 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
      601 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
      661 acaaatgtgg tatggctgat tatgatctct agtcaaggca ctatacatca aatattcctt
      721 attaacccct ttacaaatta aaaagctaaa ggtacacaat ttttgagcat agttattaat
      781 agcagacact ctatgcctgt gtggagtaag aaaaaacagt atgttatgat tataactgtt
      841 atgcctactt ataaaggtta cagaatattt ttccataatt ttcttgtata gcagtgcagc
      901 tttttccttt gtggtgtaaa tagcaaagca agcaagagtt ctattactaa acacagcatg
      961 actcaaaaaa cttagcaatt ctgaaggaaa gtccttgggg tcttctacct ttctcttctt
     1021 ttttggagga gtagaatgtt gagagtcagc agtagcctca tcatcactag atggcatttc
     1081 ttctgagcaa aacaggtttt cctcattaaa ggcattccac cactgctccc attcatcagt
     1141 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
     1201 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
     1261 tgtcacacca cagaagtaag gttccttcac aaagatccct cgaccagaca tgataagata
     1321 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     1381 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     1441 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     1501 caagtaaaac ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac
     1561 atcaaatatt ccttattaac ccctttacaa attaaaaagc taaaggtaca caatttttga
     1621 gcatagttat taatagcaga cactctatgc ctgtgtggag taagaaaaaa cagtatgtta
     1681 tgattataac tgttatgcct acttataaag gttacagaat atttttccat aattttcttg
     1741 tatagcagtg cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta
     1801 ctaaacacag catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct
     1861 acctttctct tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca
     1921 ctagatggca tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc
     1981 tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt
     2041 ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt
     2101 agtttgtcca attatgtcac accacagaag taaggttcct tcacaaagat ccctcgacca
     2161 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2221 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2281 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     2341 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag
     2401 tcaaggcact atacatcaaa tattccttat taaccccttt acaaattaaa aagctaaagg
     2461 tacacaattt ttgagcatag ttattaatag cagacactct atgcctgtgt ggagtaagaa
     2521 aaaacagtat gttatgatta taactgttat gcctacttat aaaggttaca gaatattttt
     2581 ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag
     2641 caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt
     2701 ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag
     2761 tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg
     2821 cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa
     2881 ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt
     2941 aaatctctgt aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa
     3001 agatccctcg accagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     3061 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3121 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3181 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     3241 attatgatct ctagtcaagg cactatacat caaatattcc ttattaaccc ctttacaaat
     3301 taaaaagcta aaggtacaca atttttgagc atagttatta atagcagaca ctctatgcct
     3361 gtgtggagta agaaaaaaca gtatgttatg attataactg ttatgcctac ttataaaggt
     3421 tacagaatat ttttccataa ttttcttgta tagcagtgca gctttttcct ttgtggtgta
     3481 aatagcaaag caagcaagag ttctattact aaacacagca tgactcaaaa aacttagcaa
     3541 ttctgaagga aagtccttgg ggtcttctac ctttctcttc ttttttggag gagtagaatg
     3601 ttgagagtca gcagtagcct catcatcact agatggcatt tcttctgagc aaaacaggtt
     3661 ttcctcatta aaggcattcc accactgctc ccattcatca gttccatagg ttggaatcta
     3721 aaatacacaa acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt
     3781 taccttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta
     3841 aggttccttc acaaagatcc ctcgatcgag aaaaaaaata taaaagagat ggaggaacgg
     3901 gaaaaagtta gttgtggtga taggtggcaa gtggtattcc gtaagaacaa caagaaaagc
     3961 atttcatatt atggctgaac tgagcgaaca agtgcaaaat ttaagcatca acgacaacaa
     4021 cgagaatggt tatgttcctc ctcacttaag aggaaaacca agaagtgcca gaaataacat
     4081 gagcaactac aataacaaca acggcggcta caacggtggc cgtggcggtg gcagcttctt
     4141 tagcaacaac cgtcgtggtg gttacggcaa cggtggtttc ttcggtggaa acaacggtgg
     4201 cagcagatct aacggccgtt ctggtggtag atggatcgat ggcaaacatg tcccagctcc
     4261 aagaaacgaa aaggccgaga tcgccatatt tggtgtcccc gaggatccct gtggaaaaaa
     4321 aagggacagg ataagtatga catcatcaag gaaaccctgg actactgcgc ccttcgacct
     4381 cgaaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     4441 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     4501 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     4561 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     4621 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     4681 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     4741 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     4801 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     4861 tgttctcctc ttcctcatct ccgggccttt cgacctgcat ccatctagat ctcgatcgag
     4921 cagctgaagc ttaccatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     4981 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     5041 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     5101 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     5161 gaagtgcttg acattgggga atttagcgag agcctgacct attgcatctc ccgccgtgca
     5221 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     5281 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     5341 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     5401 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     5461 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     5521 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     5581 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     5641 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     5701 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     5761 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     5821 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     5881 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     5941 cgtccgaggg caaaggaata gagtatcgat gatatcagat ccccgggatg cagaaattga
     6001 tgatctatta aacaataaag atgtccacta aaatggaagt ttttcctgtc atactttgtt
     6061 aagaagggtg agaacagagt acctacattt tgaatggaag gattggagct acgggggtgg
     6121 gggtggggtg ggattagata aatgcctgct ctttactgaa ggctctttac tattgcttta
     6181 tgataatgtt tcatagttgg atatcataat ttaaacaagc aaaaccaaat taagggccag
     6241 ctcattcctc ccactcatga tctatagatc tatagatctc tcgtgggatc attgtttttc
     6301 tcttgattcc cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc
     6361 ctgaagaacg agatcagcag cctctgttcc acatacactt cattctcagt attgttttgc
     6421 caagttctaa ttccatcaga agctggtcga gataacttcg tataatgtat gctatacgaa
     6481 gttatgtcga cagcggccgc tcgagcatgc atctagaggg cccaattcgc cctatagtga
     6541 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     6601 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     6661 ggcccgcacc gatcgccctt cccaacagtt gcgcagccta tacgtacggc agtttaaggt
     6721 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     6781 tgacacgccg gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     6841 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     6901 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     6961 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     7021 catgagatta tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca
     7081 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc
     7141 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc
     7201 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg
     7261 gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg
     7321 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     7381 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     7441 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     7501 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     7561 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     7621 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     7681 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     7741 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     7801 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     7861 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     7921 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     7981 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     8041 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     8101 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattat
     8161 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     8221 ccgcatacag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8281 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8341 tagcacgtga ggagggccac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     8401 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     8461 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     8521 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     8581 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     8641 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     8701 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctaaaact tcatttttaa
     8761 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8821 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8881 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8941 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9001 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     9061 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9121 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9181 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9241 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9301 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9361 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9421 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9481 tttttacggt tcctgggctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9541 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9601 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//
