LOCUS       sequence-439173-        9531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of GMIP mutant in mammalian cells by 
            retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Landau LM, Chaudhary N, Tien YC, Rogozinska M, Joshi S, Yao C, 
            Crowley J, Hullahalli K, Campbell IW, Waldor MK, Haigis M, Kagan JC
  TITLE     pLxIS-containing domains are biochemically flexible regulators of 
            interferons and metabolism.
  JOURNAL   Mol Cell. 2024 Jul 11;84(13):2436-2454.e10. doi: 
            10.1016/j.molcel.2024.05.030. Epub 2024 Jun 25.
  PUBMED    38925114
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05.030"; journalName: "Mol Cell"; date: 
            "2024-07-11- 11"; volume: "84"; issue: "13"; pages: "2436-2454.e10"
COMMENT     Sequence Label: pRetroX-TRE3G-GMIP P363D P364D P366D
FEATURES             Location/Qualifiers
     source          1..9531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..81
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(3040..3063)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3413..3432
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3501..4100
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3501..3520)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3997..4017
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4281..4706
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(4967..4987)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4972..5301
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5152..5287
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5323..5342
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5423..5440)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5594..6182)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5674..5693)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6353..7144,7145..7213))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6976..6995
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7214..7318)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7386..7404
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        7504..7883
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7884..8087
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8088..8263
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    8326..8525
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             8726..9142
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     9072..9094
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     9110..9126
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        9182..9531
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    9190..9208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9226..9244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9262..9280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9298..9316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9334..9352
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9370..9388
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9406..9424
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     9470..9494
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccgcca ccatggacta caaggatcac gacggtgatt acaaggacca cgacatcgac
       61 tacaaggacg acgacgacaa gatggacagt gctgaaactg agctcacacc agcccccgag
      121 ggcaggaagc gatattctga tatatttcaa agtctcgaca atctggagat ctctctcgga
      181 aacgtgactt tcgaccccct tgcaggagat ccagtaagac gcgaagatct cgagcctgat
      241 aaggcagaca ccgccaccgt tgtgaccgag gagaacagtg aggcaagcag ttggcgcgac
      301 ttgagtccag agggcccagc cccccttaca gaagaggagc ttgatcttcg cttgatcagg
      361 accaaagggg gcgtggacgc cgcccttgaa tatgccaagg catggagtcg atacgcaaag
      421 gaactgctcg cttggacaga taaaagagca aactacgaac tcgagtttgc caaatcaata
      481 atgaagttgg cagaagctgg gaaagtatcc atattgcaac aatctcagat gcctttgcaa
      541 tacatctata ctttgtttct cgagcatgac ttgtccctcg gcgcattggc tttggaaact
      601 gtagcccaac aaaagcgcga ttactatcag cctcttgcag ccaaaaggat ggaaatcgaa
      661 aagtggagaa aagagttcaa ggaacaatgg ttgaaggagc aaaaaagaat gaatgaagca
      721 gtccaggcct tgagacgctc tgagctccag tacatacagc gacgcgagga cctgcgggca
      781 cgctcacagg gttctcctga ggaccctccc tcacaggcat cccccggctc caacaaacaa
      841 caagaaagac ggcggcgcag tagggaggaa gcacaggcta aggcccacga agccgaggca
      901 ctttaccagg catgtgtgag agaggctaat tcacgccaac aagatttgga aactacaaaa
      961 cggcggatcg tctcacatgt tcgcaagctc gtgctgcagg gggatgaagt actccgacgg
     1021 gttacacttg gacttttcga acttaggggg gctcaggcag agcggggacc tcgcagcttt
     1081 tccgctctgg ccgaatgttg cgtaccattc gaacccggtc aaagatatca agaatttgta
     1141 aggactctcc agccaggtgc ccctcctgac gactccgatg ccttctgctt ccaagaattt
     1201 actgctgtag tccattcatt ccctcaggat acaaagaaga agttttctgg ccccctgccc
     1261 ccaaggcttg aagaggaagg aagtcccgag cccggtccat gggaggttgc aagcttgggt
     1321 tctcagggta ttccaggcag cgacgttgat tcagtagggg gaggctctga atctcgatcc
     1381 ttggattctc ctaccagttc tcccggcgca ggggccagaa ggctggtaaa ggccagttcc
     1441 acaggaaccg agtcctctga cgactttgaa gaacgggacc ccgaccttgg agatggaatc
     1501 gaaaatggtg taggcagtcc attcaggaag tggacattgt ctactgccgc acaaactcat
     1561 aggctgcgcc ggcttagagg tcccgcaaag tgcagggaat gtgaagcttt catggtttct
     1621 ggtaccgagt gcgaggaatg ttttttgacc tgccataagc gatgtttgga gacccttctt
     1681 atactgtgtg gccatcgacg ccttccagcc cgcatgtcac tctttggagt agacttcctc
     1741 cagctgccac gggactttcc tgaagaagtg cccttcgtca taacacggtg taccgctgag
     1801 atagaacata gggctttggg tcttcagggg atttacagag tctctgggtc ccgagtaagg
     1861 gttgaaagac tttgtcaagc cttcgaaaac gggcgagctt tggttgagct cagtggcaat
     1921 agtccccatg acattacaag cgtactcaaa aggtttctgc aggaactgac tgaccccgta
     1981 gttccattcc atctttatga tgcttttatc tctttggcaa agacactcca tgctgacccc
     2041 ggggacgatc caggtacccc aaacccatct cccgagatca tcagatcact gaaaacattg
     2101 cttgttcaac tccccgatag taattattcc accctccgcc acctcgtcgc acaccttttt
     2161 agagtcgctg ctcggttcga agaaaacaaa atgagcgcaa ataacctggg cattgtcttc
     2221 ggaccaacct tgctcaggcc cccagatggg cctcgagcaa ctggggctag ccccgtagcc
     2281 tgccttttgg acagcggcca ccaagcacag ctggtcgaat ttctcatcgt gcattacgag
     2341 caaatatttg gaatggacga gctgcctctt gcaagtgaac ctctgacaca ggaccccggc
     2401 ctggctccag cttgtttgga gtccagtccc caacaccctg catctctcct tgcccaagat
     2461 acccagccac ttacaattgc attggatagt agtccagacc ctaagcacca ttctgcactt
     2521 gagaaatgcc ccgaggttac accacccgaa ttggccacat tgcagcgaga tcagagggag
     2581 gaggaggttg aagacacacg cgacggtgct ggagatggct caagtcactg tcccgaagat
     2641 cttgcactgg gggcccagtc tcgaggtcac ttttcaaggc aaccagtaaa gtattcccgc
     2701 ggtggagtga ggcccgtgac ccaccaattg tcaagcctcg ctctcgtcgc ctcaaaactt
     2761 tgtgaagaga caccagttac cgtctccgca gtccacagag gtagtttgcg cgtaaggggc
     2821 ctgggccctg ccgccgcttg ccccgaggga agtcctcttc gccgcaaccc tctccctaaa
     2881 cattttgaga tcacacaaga aactgcacgc ttgctcagta aattgaattc agacgctgtc
     2941 tcccgcacca cttgttgcgc tgatccagaa cccgaggagt ccgaggagca cctctgataa
     3001 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     3061 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     3121 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     3181 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     3241 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     3301 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     3361 aggggtgggt ccgggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     3421 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     3481 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     3541 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     3601 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     3661 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     3721 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     3781 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     3841 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     3901 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     3961 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     4021 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     4081 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     4141 cacgacccca tgcatcggcg tctcgagata tcagtggtcc aggctctagt tttgactcaa
     4201 caatatcacc agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt
     4261 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     4321 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     4381 aggtcaggaa cagatggaac agggtcgacc ctagagaacc atcagatgtt tccagggtgc
     4441 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     4501 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     4561 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     4621 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     4681 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc
     4741 agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac
     4801 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     4861 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     4921 gccatgaccc agtcacgtag cgatagcgga gtgtagatcc ggctgtggaa tgtgtgtcag
     4981 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5041 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5101 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5161 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5221 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5281 ggaggcctag gcttttgcaa aaagcttact ggcttaacta tgcggcatca gagcagattg
     5341 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     5401 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5461 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5521 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5581 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5641 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5701 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5761 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5821 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5881 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5941 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6001 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6061 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6121 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6181 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6241 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6301 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6361 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6421 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6481 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6541 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6601 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6661 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6721 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6781 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6841 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6901 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6961 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7021 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7081 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7141 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7201 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7261 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7321 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     7381 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attagcttgg ccattgcata
     7441 cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca ttaccgccat
     7501 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     7561 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     7621 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     7681 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     7741 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     7801 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     7861 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     7921 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     7981 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     8041 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcaa taaaagagcc
     8101 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
     8161 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
     8221 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
     8281 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
     8341 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
     8401 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
     8461 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
     8521 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
     8581 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
     8641 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
     8701 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
     8761 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
     8821 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
     8881 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
     8941 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
     9001 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
     9061 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
     9121 cctcactcct tctctaggcg ccggaattga agatctcgat gaggcccttt cgtcttcact
     9181 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     9241 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     9301 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     9361 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     9421 gagaacgtat gtcgaggtag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag
     9481 tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatact t
//
