LOCUS       sequence-439199-        8877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in 
            mammalian cells by retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Landau LM, Chaudhary N, Tien YC, Rogozinska M, Joshi S, Yao C, 
            Crowley J, Hullahalli K, Campbell IW, Waldor MK, Haigis M, Kagan JC
  TITLE     pLxIS-containing domains are biochemically flexible regulators of 
            interferons and metabolism.
  JOURNAL   Mol Cell. 2024 Jul 11;84(13):2436-2454.e10. doi: 
            10.1016/j.molcel.2024.05.030. Epub 2024 Jun 25.
  PUBMED    38925114
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05.030"; journalName: "Mol Cell"; date: 
            "2024-07-11- 11"; volume: "84"; issue: "13"; pages: "2436-2454.e10"
COMMENT     Sequence Label: pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)
FEATURES             Location/Qualifiers
     source          1..8877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..39
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1654..1707
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1708..1737
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        2327..2826
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2362..2385)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2759..2778
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2847..3446
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2847..2866)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3343..3363
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3627..4052
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(4155..4177)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4313..4333)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4318..4647
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4498..4633
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4669..4688
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4769..4786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4940..5528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5020..5039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5699..6490,6491..6559))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6322..6341
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6560..6664)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6732..6750
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        6850..7229
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7230..7433
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7434..7609
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    7672..7871
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             8072..8488
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     8418..8440
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     8456..8472
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        8528..8877
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    8536..8554
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8572..8590
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8608..8626
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8644..8662
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8680..8698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8716..8734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8752..8770
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8816..8840
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccgcca ccatggatta taaagacgat gacgacaaaa tgtccctgag ccgctccgaa
       61 gaaatgcaca gattgactga gaatgtatat aaaactatta tggagcaatt caacccctca
      121 cttagaaact ttatagctat gggtaagaat tacgagaagg ccctcgcagg tgtgaccttc
      181 gctgctaaag ggtacttcga tgctctcgtt aaaatgggcg agttggcaag tgaatcccag
      241 ggttctaagg agctcggaga cgtcctgttc caaatggccg aggtccaccg gcaaatacag
      301 aatcaattgg aagaaactct caagtctttt cacaacgaac tgttgaccca gctggagcaa
      361 aaggtcgagc ttgactctcg gtatctttcc gccgcactta aaaagtacca aacagagcaa
      421 aggtctaaag gagacgctct cgacaaatgc caggctgaac tgaagaaatt gcgcaaaaag
      481 tcccagggct ccaaaaaccc tcaaaaatat tccgataaag aactccaata cattgatgct
      541 atctctaaca aacaggggga acttgagaat tatgtgtcag acggctataa gactgcactt
      601 accgaggagc gaagacggtt ttgctttctt gtggaaaagc agtgcgcagt ggctaagaat
      661 agcgctgctt atcattccaa agggaaggaa ctgttggcac aaaagttgcc actctggcaa
      721 caagcttgtg ctgatccaaa taaaattcct gatcgagctg tgcagttgat gcaacagatg
      781 gcaaactcaa acgggtctat cctcccatca gccttgagcg cttcaaaatc taatcttgtg
      841 atctcagacc ctatacccgg cgcaaagccc ttgcctgtcc ccccagaact tgctcctttc
      901 gttgggagga tgtccgccca agaaaacgtc cctgtgatga atggggtagc cgggcccgac
      961 tccgaggact acaacccatg ggctgataga aaggccgcac aacccaaatc tctctcaccc
     1021 ccacagagtc aatctaaact ctctgattca tatagcaata ctcttcctgt taggaaatcc
     1081 gtaaccccta aaaacagcta cgccaccacc gagaataaaa ctttgcctcg cagctctagt
     1141 atggcagctg gtctcgagag aaatggtagg atgagggtaa aagcaatttt cagtcatgct
     1201 gccggcgaca attctaccct cctgtccttt aaggagggtg atttgatcac actgttggtc
     1261 cctgaggctc gagatggctg gcattacggc gagagcgaaa agacaaagat gcgcgggtgg
     1321 tttccattta gctatacacg ggtacttgat tcagatggct ccgaccgact tcacatgtcc
     1381 ctgcagcaag gaaaatcctc aagtaccggc aatcttttgg acaaagacga tctggccctc
     1441 cctccacctg attacggaac ttctagtcgc gcattcccaa cccagaccgc aggaacattt
     1501 aaacaacgcc catacagcgt ggccgttcct gccttttccc aaggtctgga cgattacgga
     1561 gctcggagcg tttctcggaa tccctttgct aacgtccatc tcaaaccaac cgtcaccaat
     1621 gaccgcagtg ctcccctgct ttctggaagc ggggagggca ggggttccct tctgacctgt
     1681 ggagatgtcg aagagaatcc aggtccagag caaaagttga tctccgaaga agatcttatg
     1741 caaacaatca agtgcgtagt cgtcggggac gtagccgtgg gaaaaacatg cttgctcata
     1801 tcctatacaa ccaataagtt tccaagcgag tacgtgccta ctgtgtttga taattatgcc
     1861 gtcacagtca tgatcggggg cgagccctat acactgggac tgttcgatac tgcaggtcaa
     1921 gaggactacg accgccttcg acccctgtca tatccccaaa ccgatgtatt tttggtatgt
     1981 ttctctgtcg tgagtccttc aagctttgaa aatgttaaag agaagtgggt accagaaatt
     2041 actcatcact gtcctaaaac ccctttcctg cttgtgggca cccagattga tctgcgagac
     2101 gatccatcaa caatagagaa attggcaaaa aataaacaaa agcctattac accagagact
     2161 gccgaaaaac tcgcacgcga tcttaaagca gtgaaatacg tcgaatgctc agctttgact
     2221 cagaaaggac ttaagaacgt ctttgacgaa gcaatcctgg ccgctctcga acccccagag
     2281 cccaaaaaat cccggaggtg tgtccttcta tgataagaat tctaccgggt aggggaggcg
     2341 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     2401 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     2461 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     2521 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     2581 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     2641 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     2701 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     2761 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     2821 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     2881 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     2941 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     3001 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     3061 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     3121 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     3181 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     3241 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     3301 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     3361 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     3421 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     3481 ggagcgcacg accccatgca tcggcgtctc gagatatcag tggtccaggc tctagttttg
     3541 actcaacaat atcaccagct gaagcctata gagtacgagc catagataaa ataaaagatt
     3601 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     3661 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
     3721 agatcaaggt caggaacaga tggaacaggg tcgaccctag agaaccatca gatgtttcca
     3781 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     3841 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     3901 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
     3961 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     4021 gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc
     4081 ctgcccaggg accaccgacc caccaccggg aggtaagctg gctgcctcgc gcgtttcggt
     4141 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     4201 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     4261 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt agatccggct gtggaatgtg
     4321 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4381 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4441 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4501 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4561 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4621 ttttttggag gcctaggctt ttgcaaaaag cttactggct taactatgcg gcatcagagc
     4681 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     4741 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4801 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4861 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4921 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4981 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5041 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5101 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5161 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5221 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5281 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5341 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     5401 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5461 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5521 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5581 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5641 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5701 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5761 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5821 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5881 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5941 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6001 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6061 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6121 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6181 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6241 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6301 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6361 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6421 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6481 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6541 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6601 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6661 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     6721 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta gcttggccat
     6781 tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac
     6841 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     6901 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     6961 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     7021 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     7081 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     7141 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     7201 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     7261 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     7321 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     7381 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcaataaa
     7441 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
     7501 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
     7561 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
     7621 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
     7681 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
     7741 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
     7801 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
     7861 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
     7921 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
     7981 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     8041 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     8101 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     8161 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     8221 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     8281 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     8341 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     8401 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     8461 tccagccctc actccttctc taggcgccgg aattgaagat ctcgatgagg ccctttcgtc
     8521 ttcactcgag tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
     8581 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
     8641 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
     8701 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
     8761 gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg cgcctataaa agcagagctc
     8821 gtttagtgaa ccgtcagatc gcctggagca attccacaac acttttgtct tatactt
//
