LOCUS       sequence-439272-        8420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  equimolar expression of ymStayGold and mCherry in budding yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Botman D, de Groot DH, Wehrens M, van Rossum K, Yska S, van der 
            Kieft B, Albert V, Minghetti N, Goedhart J, Teusink B
  TITLE     Side-by-side systematic characterization of novel FPs in budding 
            yeast
  JOURNAL   bioRxiv 2025.06.27.661975; doi: 
            https://doi.org/10.1101/2025.06.27.661975
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.27.661975; doi: https://doi.org/10.1101/2025.06.27.661975"
COMMENT     Sequence Label: pDRF1 ymStayGold-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..8420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             831..1508
                     /codon_start=1
                     /product="bright and highly photostable monomeric green 
                     fluorescent protein (Ando et al., 2023)"
                     /label=mStayGold(J)
                     /note="includes multiple mutations plus an N-terminal 
                     iinker sequence"
                     /translation="MVSTGEELFTGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
                     KYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGS
                     IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMDVSLPNEVQHIPIDDGVECTVTL
                     QYPLLSDESKCVEAYQNTIIKPLHNQPAPDVPFHWIRKQYTQSKDDTEERDHIIQSETL
                     EAHL"
     CDS             1515..1568
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1584..1593
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1590..2300
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1735..1753)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2003..2022
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2310..2334)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2396..2415)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2397..2415)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2563..2750
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2618..2642)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2762..2779)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2762..2778)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2771..2793)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3214..4560
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4671..5474)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5475..5690)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5715..5733)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5801..5905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5906..5974,5975..6766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6124..6143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6937..7525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7426..7445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7679..7696
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7813..7834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7849..7854,7855..7872,7873..7879)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7887..7903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7892..7914
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7911..7927
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7911..7927
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7957..8412)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8094..8115)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8306..8325
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atggtttcta
      841 ctggtgaaga attgtttact ggtgttgttc cattcaagtt ccaattaaag ggtactatta
      901 acggtaagtc tttcactgtt gaaggtgaag gtgaaggtaa ctctcatgaa ggttctcata
      961 agggtaagta cgtttgtact tctggtaagt tgccaatgtc ttgggctgct ttaggtactt
     1021 ctttcggtta cggtatgaag tactacacta agtacccatc tggtttgaag aactggttcc
     1081 atgaagttat gcctgaaggt ttcacttacg atagacatat tcaatacaag ggtgatggtt
     1141 ctattcatgc taagcatcaa catttcatga agaacggtac ttaccataac attgttgaat
     1201 tcactggtca agatttcaag gaaaactccc cagttttgac tggtgatatg gatgtttctt
     1261 tgccaaacga agttcaacat attccaattg atgatggtgt tgaatgtact gttactttgc
     1321 aatacccatt gttgtctgat gaatctaagt gtgttgaagc ctaccaaaat actattatta
     1381 agccattgca taaccaacca gctccagatg ttccattcca ctggattaga aaacaataca
     1441 ctcaatctaa ggatgatact gaagaaagag atcatattat tcaatctgaa actttggaag
     1501 ctcatttgtc cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
     1561 ccggccctgg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
     1621 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1681 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1741 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1801 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1861 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1921 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1981 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     2041 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     2101 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     2161 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     2221 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     2281 tggacgagct gtacaagtaa agcggccgca cccagctttc ttgtacaaag tggtgtagtt
     2341 aattcatgat ggccgctgca ggtcgacctc gagggggggc ccggtaccca attcgcccta
     2401 tagtgagtcg tattacgcgc ggatccagct ttggacttct tcgccagagg tttggtcaag
     2461 tctccaatca aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag
     2521 ggtcaaatcg ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt
     2581 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac
     2641 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg
     2701 ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg catgccaatt
     2761 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     2821 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     2881 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     2941 ttacgcatct gtgcggtatt tcacaccgca taatcggatc gtacttgtta cccatcattg
     3001 aattttgaac atccgaacct ggaagttttc cctgaaacag atagtatatt tgaacctgta
     3061 taataatata tagtctagcg ctttacggaa gacaatgtat gtatttcggt tcctggagaa
     3121 actattgcat ctattgcata ggtaatcttg cacgtcgcat ccccggttca ttttctgcgt
     3181 ttccatcttg cacttcaata gcatatcttt gttaacgaag catctgtgct tcattttgta
     3241 gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     3301 acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt
     3361 ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     3421 tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt
     3481 cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga
     3541 ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt
     3601 aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag
     3661 cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag
     3721 ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga
     3781 aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt
     3841 gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta
     3901 tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa
     3961 atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat
     4021 agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg
     4081 gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg
     4141 cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagctag
     4201 agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa
     4261 tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg
     4321 cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac
     4381 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc
     4441 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg
     4501 ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc
     4561 gatccgatga taagctgtca aacatgagaa ttgggtaata actgatataa ttaaattgaa
     4621 gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc
     4681 tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc
     4741 ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta
     4801 gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa
     4861 cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt
     4921 tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta
     4981 gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct
     5041 ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc
     5101 accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag
     5161 tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa
     5221 ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc
     5281 aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc
     5341 agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc
     5401 atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat
     5461 gtagctttcg acatgattta tcttcgtttc ctgcatgttt ttgttctgtg cagttgggtt
     5521 aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata taccaatcta
     5581 agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt
     5641 caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa aagctaattc
     5701 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     5761 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5821 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5881 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5941 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6001 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6061 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6121 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6181 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6241 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6301 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6361 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6421 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6481 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6541 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6601 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6661 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6721 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6781 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6841 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6901 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6961 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7021 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7081 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7141 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7201 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7261 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7321 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7381 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7441 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7501 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7561 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7621 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7681 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     7741 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     7801 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     7861 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     7921 ctatgaccat gattacgcca agcttaccgc atcaggaaat tgtaagcgtt aatattttgt
     7981 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     8041 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     8101 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     8161 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     8221 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     8281 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     8341 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     8401 tacagggcgc gtccattcgc
//
