LOCUS       sequence-439805-        7747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            SOHLH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Pierson Smela M, Kramme CC, Fortuna PRJ, Wolf B, Srikar Kavirayuni 
            V, Adams J, Ma C, Velychko S, Widocki U, Goel S, Chen T, Vincoff S, 
            Dong E, Kohman RE, Kobayashi M, Shioda T, Church GM, Chatterjee P
  TITLE     Rapid Human Oogonia-like Cell Specification via Combinatorial 
            Transcription Factor-Directed Differentiation
  JOURNAL   bioRxiv 2022.07.11.499564
REFERENCE   2  (bases 1 to 7747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.11.499564"
COMMENT     Sequence Label: pPB-cT3G-cERH-SOHLH1
FEATURES             Location/Qualifiers
     source          1..7747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     160..177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    383..404
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(419..424,425..442,443..449)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    457..473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     462..484
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     481..497
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     481..497
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   523..585
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    761..842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(959..1984)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVHRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1985..2041)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2051..2794)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(2795..2818)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2818..2827
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2847..3058)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        3059..3438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    3647..3665
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3683..3701
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3719..3737
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3755..3773
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3791..3809
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3827..3845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3863..3881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3908..3932
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3998..4022
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4025..4034
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5019..5043)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5045..5071
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    5085..5219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5491..5525
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5536..5553)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5536..5552)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5545..5567)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5761..5780)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5882..5904
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5943..5961)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6029..6133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6134..6202,6203..6994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6352..6371)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(7165..7747,1..6)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7654..7673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
       61 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      121 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      181 gcggatgagc gcccaatacg caaaccgcct ctcaccgcgc gttggccgat tcattaatgc
      241 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aaaccgcctc
      301 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      361 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      421 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      481 caggaaacag ctatgaccat gattacgcca aggtcgactt aaccctagaa agataatcat
      541 attgtgacgt acgttaaaga taatcatgcg taaaattgac gcatgtgttt tatcggtctg
      601 tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat ttacacttac
      661 atactaataa taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaac
      721 aaaaactcaa aatttcttct ataaagtaac aaaactttta gcagtgaaaa aaatgcttta
      781 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      841 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      901 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctggagatct
      961 attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     1021 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     1081 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     1141 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     1201 gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     1261 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     1321 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     1381 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     1441 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     1501 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccacgta gtgtattgac
     1561 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
     1621 tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt
     1681 cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt
     1741 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgatgaacat
     1801 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
     1861 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
     1921 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
     1981 tcatggggcc ggggttctcc tccacgtcgc cggcctgctt cagcaggctg aagttggtgg
     2041 cgccgctgcc cccggggagc atgtcaaggt caaaatcgtc aagagcgtca gcaggcagca
     2101 tatcaaggtc aaagtcgtca agggcatcgg ctgggagcat gtctaagtca aaatcgtcaa
     2161 gggcgtcggt cggcccgccg ctttcgcact ttagctgttt ctccaggcca catatgatta
     2221 gttccaggcc gaaaaggaag gcaggttcgg ctccctgccg gtcgaacagc tcaattgctt
     2281 gtttcagaag tgggggcata gaatcggtgg taggtgtctc tctttcctct tttgctactt
     2341 gatgctcctg ttcctccaat acgcagccca gtgtaaagtg gcccacggcg gacagagcgt
     2401 acagtgcgtt ctccagggag aagccttgct gacacaggaa cgcgagctga ttttccaggg
     2461 tttcgtactg tttctctgtt gggcgggtgc cgagatgcac tttagccccg tcacgatgtg
     2521 agaggagagc acagcggtat gacttggcgt tgttccgcag aaagtcttgc catgactcgc
     2581 cttccagggg gcaggagtgg gtatgatgcc tgtccagcat ctcgattggc agggcatcga
     2641 gcagggcccg cttgttcttc acgtgccagt acagggtagg ctgctcaact cccagctttt
     2701 gagcgagttt ccttgtcgtc aggccttcga taccgactcc attgagtaat tccagagcag
     2761 agtttatgac tttgctcttg tccagtctag acatcttatc gtcatcgtct ttgtaatcca
     2821 tggtggcgga tcccgcgtca cgacacctgt gttctggcgg caaacccgtt gcgaaaaaga
     2881 acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg aaaaaggcgg
     2941 agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta ggcaccggtt
     3001 caattgccga cccctccccc caacttctcg ggcactgtgg gccatgtgcg ctctgcccca
     3061 tggtaatagc catgactaat acgtagatgt actgccaagt aggaaagtgc cataaggtca
     3121 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     3181 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     3241 attgacgtca atggaaagtg cctattggcg ttactatggg aacatacgtc attattgacg
     3301 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     3361 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     3421 tagtcaataa tcaatgtcat aagctctaat cgcataatcg agctaagagc gattaatatg
     3481 cttagctagc ttagctaagc tagatttctc tattctccca caatatatag cagctattag
     3541 cttagcagtc cgaatacgat cgtccacgat ccataagcgt aacacatttg agtagcgact
     3601 gacgggcacc ggagccactc gagtggaatt atcacctcga gtttactccc tatcagtgat
     3661 agagaacgta tgaagagttt actccctatc agtgatagag aacgtatgca gactttactc
     3721 cctatcagtg atagagaacg tataaggagt ttactcccta tcagtgatag agaacgtatg
     3781 accagtttac tccctatcag tgatagagaa cgtatctaca gtttactccc tatcagtgat
     3841 agagaacgta tatccagttt actccctatc agtgatagag aacgtataag cttgcctata
     3901 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     3961 acctccatag aagacaccgg gaccgatcca gcctaccaca agtttgtaca aaaaagcagg
     4021 cttagccacc atggcgtccc ggtgctccga gccctacccg gaggtctcca gaatccccac
     4081 cgtcagggga tgcaacggct ctctatctgg tgcactatct tgttgtgaag attcagcaca
     4141 aggttctggt ccgcccaagg cccctacggt ggccgagggt cccagctcct gccttcggcg
     4201 gaacgtgatc agcgagaggg agcgcaggaa gcggatgtcg ttgagctgtg agcgtctgcg
     4261 ggccctgctg ccccagttcg atggccggcg ggaggacatg gcctcggtcc tggagatgtc
     4321 tgtgcagttc ctgcggcttg ccagcgccct ggggcccagt caggagcagc acgctattct
     4381 tgcttcctcc aaggaaatgt ggcactcgtt gcaggaggat gttttacagt tgacgttgtc
     4441 gagtcagatt caagcaggtg tgccagaccc tgggacggga gcgtccagcg ggactcgaac
     4501 cccagatgtg aaggcgtttc tggaaagtcc ttggtccctg gatccagcgt cggccagccc
     4561 agagcccgtg ccgcacatcc ttgcgtcctc caggcagtgg gaccccgcga gctgcacgtc
     4621 cctgggcacg gacaagtgtg aggcactgtt ggggctgtgc caggtgcggg gtgggctgcc
     4681 ccctttctca gaaccttcca gcctggtgcc gtggccccca ggccggagtc ttcctaaggc
     4741 tgtgaggcca cccctgtcct ggcctccgtt ctcgcagcag cagaccttgc ccgtgatgag
     4801 cggggaggca cttggttggc tgggccaggc tggttccctg gccatggggg ctgcacctct
     4861 gggggagcca gccaaggagg accccatgct ggcgcaggag gccgggtctg cgttggggtc
     4921 tgatgtggac gatgggacgt ccttcctgct gactgctggt cccagctcgt ggccgggtga
     4981 gtggggccct gggttcaggg ctggtccccc tgcgtaatac ccagctttct tgtacaaagt
     5041 ggtataccca tacgatgttc cagattacgc ttaactttct ccgcgatcca gacatgataa
     5101 gatacattga tgagtttgga caaaccaaaa ctagaatgca gtgaaaaaaa tgccttattt
     5161 gtgaaatttg tgatgctatt gccttatttg taaccattat aagctgcaat aaacaagtta
     5221 tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa
     5281 tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt
     5341 gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct
     5401 cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat
     5461 ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc
     5521 tagggttaag aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5581 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5641 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     5701 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     5761 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     5821 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccatgtc
     5881 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacacgc gagttcgaaa
     5941 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     6001 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6061 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6121 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6181 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6241 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6301 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6361 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6421 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6481 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6541 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6661 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6721 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6781 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6841 tgagcgtggt tcacgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6961 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7021 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7081 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7741 gagccta
//
