LOCUS       sequence-439825-        2232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV
            capsid in mice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Tabebordbar M, Lagerborg KA, Stanton A, King EM, Ye S, Tellez L, 
            Krunnfusz A, Tavakoli S, Widrick JJ, Messemer KA, Troiano EC, 
            Moghadaszadeh B, Peacker BL, Leacock KA, Horwitz N, Beggs AH, Wagers
            AJ, Sabeti PC
  TITLE     Directed evolution of a family of AAV capsid variants enabling 
            potent muscle-directed gene delivery across species.
  JOURNAL   Cell. 2021 Sep 16;184(19):4919-4938.e22. doi: 
            10.1016/j.cell.2021.08.028. Epub 2021 Sep 9.
  PUBMED    34506722
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.08.028"; journalName: "Cell"; date: 
            "2021-09-16- 16"; volume: "184"; issue: "19"; pages: "4919-4938.e22"
COMMENT     Sequence Label: kiCAP-AAV-MyoAAV2A
FEATURES             Location/Qualifiers
     source          1..2232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2074..2085
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggctgccg atggttatct tccagattgg ctcgaggaca accttagtga aggaattcgc
       61 gagtggtggg ctttgaaacc tggagcccct caacccaagg caaatcaaca acatcaagac
      121 aacgctcgag gtcttgtgct tccgggttac aaataccttg gacccggcaa cggactcgac
      181 aagggggagc cggtcaacgc agcagacgcg gcggccctcg agcacgacaa ggcctacgac
      241 cagcagctca aggccggaga caacccgtac ctcaagtaca accacgccga cgccgagttc
      301 caggagcggc tcaaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag
      361 gccaaaaaga ggcttcttga acctcttggt ctggttgagg aagcggctaa gacggctcct
      421 ggaaagaaga ggcctgtaga gcagtctcct caggaaccgg actcctccgc gggtattggc
      481 aaatcgggtg cacagcccgc taaaaagaga ctcaatttcg gtcagactgg cgacacagag
      541 tcagtcccag accctcaacc aatcggagaa cctcccgcag ccccctcagg tgtgggatct
      601 cttacaatgg cttcaggtgg tggcgcacca gtggcagaca ataacgaagg tgccgatgga
      661 gtgggtagtt cctcgggaaa ttggcattgc gattcccaat ggctggggga cagagtcatc
      721 accaccagca cccgaacctg ggccctgccc acctacaaca atcacctcta caagcaaatc
      781 tccaacagca catctggagg atcttcaaat gacaacgcct acttcggcta cagcaccccc
      841 tgggggtatt ttgacttcaa cagattccac tgccacttct caccacgtga ctggcagcga
      901 ctcatcaaca acaactgggg attccggcct aagcgactca acttcaagct cttcaacatt
      961 caggtcaaag aggttacgga caacaatgga gtcaagacca tcgccaataa ccttaccagc
     1021 acggtccagg tcttcacgga ctcagactat cagctcccgt acgtgctcgg gtcggctcac
     1081 gagggctgcc tcccgccgtt cccagcggac gttttcatga ttcctcagta cgggtatctg
     1141 acgcttaatg atggaagcca ggccgtgggt cgttcgtcct tttactgcct ggaatatttc
     1201 ccgtcgcaaa tgctaagaac gggtaacaac ttccagttca gctacgagtt tgagaacgta
     1261 cctttccata gcagctacgc tcacagccaa agcctggacc gactaatgaa tccactcatc
     1321 gaccaatact tgtactatct ctcaaagact attaacggtt ctggacagaa tcaacaaacg
     1381 ctaaaattca gtgtggccgg acccagcaac atggctgtcc agggaagaaa ctacatacct
     1441 ggacccagct accgacaaca acgtgtctca accactgtga ctcaaaacaa caacagcgaa
     1501 tttgcttggc ctggagcttc ttcttgggct ctcaatggac gtaatagctt gatgaatcct
     1561 ggacctgcta tggccagcca caaagaagga gaggaccgtt tctttccttt gtctggatct
     1621 ttaatttttg gcaaacaagg aactggaaga gacaacgtgg atgcggacaa agtcatgata
     1681 accaacgaag aagaaattaa aactactaac ccggtagcaa cggagtccta tggacaagtg
     1741 gccacaaacc accaggggcc gggtagagga gaccagacta cgttggcaca ggcgcagacc
     1801 ggctgggttc aaaaccaagg aatacttccg ggtatggttt ggcaggacag agatgtgtac
     1861 ctgcaaggac ccatttgggc caaaattcct cacacggacg gcaactttca cccttctccg
     1921 ctgatgggag ggtttggaat gaagcacccg cctcctcaga tcctcatcaa aaacacacct
     1981 gtacctgcgg atcctccaac ggccttcaac aaggacaagc tgaactcttt catcacccag
     2041 tattctactg gccaagtcag cgtggagatc gagtgggagc tgcagaagga aaacagcaag
     2101 cgctggaacc cggagatcca gtacacttcc aactattaca agtctaataa tgttgaattt
     2161 gctgttaata ctgaaggtgt atatagtgaa ccccgcccca ttggcaccag atacctgact
     2221 cgtaatctgt aa
//
