LOCUS       sequence-440072-        1704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of ZFPL1-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ugur B, Schueder F, Shin J, Hanna MG, Wu Y, Leonzino M, Su M, McAdow
            AR, Wilson C, Postlethwait J, Solnica-Krezel L, Bewersdorf J, De 
            Camilli P
  TITLE     VPS13B is localized at the cis-trans Golgi complex interface and is 
            a functional partner of FAM177A1.
  JOURNAL   bioRxiv [Preprint]. 2023 Dec 18:2023.12.18.572081. doi: 
            10.1101/2023.12.18.572081.
  PUBMED    38187698
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Dec 18:2023.12.18.572081. doi: 
            10.1101/2023.12.18.572081."
COMMENT     Sequence Label: ZFPL1-GFP
FEATURES             Location/Qualifiers
     source          1..1704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(988..990,991..1704)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             988..1701
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1030..1051)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1291..1310)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1638..1659
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggggcttt gtaagtgccc caagagaaag gtgaccaacc tgttctgctt cgaacatcgg
       61 gtcaacgtct gcgagcactg cctggtagcc aatcacgcca agtgcatcgt ccagtcctac
      121 ctgcaatggc tccaagatag cgactacaac cccaattgcc gcctgtgcaa catacccctg
      181 gccagccgag agacgacccg ccttgtctgc tatgatctct ttcactgggc ctgcctcaat
      241 gaacgtgctg cccagctacc ccgaaacacg gcacctgccg gctatcagtg ccccagctgc
      301 aatggcccca tcttcccccc aaccaacctg gctggccccg tggcctccgc actgagagag
      361 aagctggcca cagtcaactg ggcccgggca ggactgggcc tccctctgat cgatgaggtg
      421 gtgagcccag agcccgagcc cctcaacacg tctgacttct ctgactggtc tagttttaat
      481 gccagcagta cccctggacc agaggaggta gacagcgcct ctgctgcccc agccttctac
      541 agccaggccc cccggccccc agcttcccca ggccggcccg agcagcacac agtgatccac
      601 atgggcaatc ctgagccctt gactcacgcc cctaggaagg tgtatgatac gcgggatgat
      661 gaccggacac caggcctcca tggagactgt gacgatgaca agtaccgacg tcggccggcc
      721 ttgggttggc tggcccggct gctaaggagc cgggctgggt ctcggaagcg gccgctgacc
      781 ctgctccagc gggcggggct gctgctactc ttgggactgc tgggcttcct ggccctcctt
      841 gccctcatgt ctcgcctagg ccgggccgca gctgacagcg atcccaacct ggacccactc
      901 atgaaccctc acatccgcgt gggcccctcc ggatccacta gtccagtgtg gtggaattct
      961 gcagatatcc agcacagtgg cggccgcatg agcaagggcg aggagctgtt caccggggtg
     1021 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1081 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1141 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     1201 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1261 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1321 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1381 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1441 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1501 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1561 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     1621 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactcac
     1681 ggcatggacg agctgtacaa gtaa
//
