LOCUS       sequence-440678-        1178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression GST fusion CD28 CAR hinge (I114-P152) in Escherichia 
            coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Folimonova V, Chen X, Negi H, Schwieters CD, Li J, Byrd RA, Taylor 
            N, Youkharibache P, Walters KJ
  TITLE     CD28 hinge used in chimeric antigen receptor (CAR) T-cells exhibits 
            local structure and conformational exchange amidst global disorder.
  JOURNAL   Commun Biol. 2024 Aug 31;7(1):1072. doi: 10.1038/s42003-024-06770-w.
  PUBMED    39217198
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-08-31"; volume: "7(1):1072. doi"; pages: " 
            10.1038/s42003-024-06770-w"
COMMENT     Sequence Label: pGEX6P1-CD28 CAR hinge (I114 – P152)
FEATURES             Location/Qualifiers
     source          1..1178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(175..198)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(205..858)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(225..247)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(870..892)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    881..897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(905..911,912..927,928..933))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 acgcgcgagg cagatcgtca gtcagtcacg atgcggccgc tcgagttacg gtttgcttgg
       61 acccgggaac aacggggacg ggcacaggtg cttaccctta acatggataa tggtgccatt
      121 gcttttctcg ttgtctagat aaggtggggg gtacattact tctatggatc ccaggggccc
      181 ctggaacaga acttccagat ccgattttgg aggatggtcg ccaccaccaa acgtggcttg
      241 ccagccctgc aaaggccatg ctatatactt gctggatttc aagtacttat caatttgtgg
      301 gatagcttca atacgttttt taaaacaaac taattttggg aacgcatcca ggcacattgg
      361 gtccatgtat aaaacaacat caagagcgtc atacaacatg aagtcaggat gggttacatg
      421 atcaccattt aaatatgttt tatgacataa acgatcttcg aacattttca gcatttcagg
      481 tagcttgcta agaaaatcaa ctttgagagt ttcaaagtct ttactatatg caattctcga
      541 aacaccgtat ctaatatcca aaaccgctcc ttcaagcatt gaaatctctg cacgctcttt
      601 tggacaacca cccaacatgt tgtgcttgtc agctatataa cgtatgatgg ccatagactg
      661 tgttaattta acatcaccat caatataata aggaagattg ggaaactcca aacccaattc
      721 aaactttttg tttcgccatt tatcaccttc atcgcgctca tacaaatgct cttcatattt
      781 ttcttcaaga tattccaaaa gaagtcgagt gggttgcaca aggcccttaa ttttccaata
      841 acctagtata ggggacatga atactgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      901 cacacattat acgagccgat gattaattgt caacagctca tttcagaata tttgccagaa
      961 ccgttatgat gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt gagcgacacg
     1021 aattatgcag tgatttacga cctgcacagc cataccacag cttccgatgg ctgcctgacg
     1081 ccagaagcat tggtgcaccg tgcagtcgaa aagctaattc cacgccatca aaataatcgc
     1141 gtntggcctt ctgtagcagc ttcatcacnt taaaggga
//
