LOCUS       sequence-440841-       11030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NRAS G12V and ASK1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Odawara T, Yamauchi S, Ichijo H
  TITLE     Apoptosis signal-regulating kinase 1 promotes inflammation in 
            senescence and aging.
  JOURNAL   Commun Biol. 2024 Jun 5;7(1):691. doi: 10.1038/s42003-024-06386-0.
  PUBMED    38839869
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-06-5"; volume: "7(1):691. doi"; pages: " 
            10.1038/s42003-024-06386-0"
COMMENT     Sequence Label: pT/Caggs-NRAS G12V-IRES-ASK1
FEATURES             Location/Qualifiers
     source          1..11030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..658
                     /label=chicken beta-actin promoter
     intron          659..1675
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1599..1621
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1683..1702
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        complement(1748..1766)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1749..1766)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1800..1817
                     /codon_start=1
                     /product="epitope tag from middle T antigen of mouse 
                     polyoma virus"
                     /label=Glu-Glu tag
                     /translation="EYMPME"
     misc_feature    join(2388..2962,2963..2965,2966..2974)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2555..2572)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2782..2801
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(7125..7142)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7131..7355
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7809..7829)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7809..7827)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7848..7864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7848..7864)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7861..7883)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7872..7888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7896..7902,7903..7920,7921..7926))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7941..7962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8079..8096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8250..8838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8330..8349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9009..9800,9801..9869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9632..9651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9870..9974)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10001..10456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10088..10107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10298..10319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10582..10604
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10596..10613
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10597..10613
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10623..10642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10623..10641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(join(11015..11030,1))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(11016..11030,1..2))
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      541 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
      841 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
      901 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     1021 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     1081 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     1141 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     1201 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     1261 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     1321 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     1381 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1501 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     1561 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1621 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1681 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcc tcgagggggg
     1741 gccctattct atagtgtcac ctaaatgcta gagctcgctg atccaccatg gaggaagagg
     1801 aatatatgcc tatggaaatg actgagtaca aactggtggt ggttggagca gttggtgttg
     1861 ggaaaagcgc actgacaatc cagctaatcc agaaccactt tgtagatgaa tatgatccca
     1921 ccatagagga ttcttacaga aaacaagtgg ttatagatgg tgaaacctgt ttgttggaca
     1981 tactggatac agctggacaa gaagagtaca gtgccatgag agaccaatac atgaggacag
     2041 gcgaaggctt cctctgtgta tttgccatca ataatagcaa gtcatttgcg gatattaacc
     2101 tctacaggga acagattaag cgagtaaaag actcggatga tgtacctatg gtgctagtgg
     2161 gaaacaagtg tgatttgcca acaaggacag ttgatacaaa acaagcccac gaactggcca
     2221 agagttacgg gattccattc attgaaacct cagccaagac cagacagggt gttgaagatg
     2281 ctttttacac actggtaaga gaaatacgcc agtaccgaat gaaaaaactc aacagcagtg
     2341 atgatgggac tcagggttgt atgggattgc catgtgtggt gatgtaaccc ctctccctcc
     2401 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     2461 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     2521 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     2581 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     2641 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     2701 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     2761 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     2821 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca
     2881 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     2941 ctttgaaaaa cacgatgata atatggccac aaccatgggc accgaagccg gcgagggcat
     3001 cactttctcc gtgccgccct ttgcgtccgt gggcttttgc accatcccgg agggcggcag
     3061 ctgccggagg ggaggaggag cggcgacggc ggcggaggga gagccgagcc tgcagccctt
     3121 gcttgtgccg ccgccgccgc caccgccggg cagtttctgg aacgtggaga gcgcggcggc
     3181 tcccggcacc agctgcccta ccaccgcccc cgggagcagc gccacccgag gccggggcaa
     3241 ctctggcagc gggggcggcc ggcgtaccac cgtggcgtat gtgatcaatg aggcgagcca
     3301 ggggcagctg gtggtggcgg agagcgaggc cctgcagagc ctgcgggagg cgtgcgaggc
     3361 ggtgggcgcc accctggaga ccctgcattt cgggaagctg gacttcgggg aaaccgccgt
     3421 gctggaccgt ttttacaacg cagatattgc tgtggtggag atgagcgacg ccttccggca
     3481 gccttccttg ttttaccatc ttggagtgcg agaaagcttc agtatggcca acaacatcat
     3541 cctctactgc gatactaatt ccgattcact ccagtccctg agggaaatta tttgccagaa
     3601 gaatactgtg tgcaccggga actacacctt catcccctac atggtgacac cacacaacaa
     3661 ggtctactgc tgtgacagca gcttcatgaa gggcctgact gagctcatgc agcctaactt
     3721 cgagctcctg ctggggccca tctgcttacc cctcgtggac cgttttgttc agcttttgaa
     3781 ggtggcgcaa gcgagttcca gccagtactt ccgggaatcc atactcagcg atatcaggaa
     3841 agcacggaac ctgtacactg gtaaagaatt ggcagctgaa ttggcgagga tccggcaacg
     3901 ggtagacaat attgaagttt tgacagcaga cattgttatc aacctgttgc tttcctacag
     3961 agacatccag gactatgact ccatagtgaa gctggtagag acgttagaaa aacttccaac
     4021 cttcgacttg gcctcgcacc accacgtgaa gtttcattac gcatttgcac tgaacaggag
     4081 aaatctccct ggagacagag caaaagctct tgacatcatg atccccatgg tacaaagtga
     4141 agagcaagtt gcttcggata tgtattgtct cgttggccga atctacaaag atatgttttt
     4201 ggattcaaat ttcactgaca cggaaagcag ggaccatgga gcatcttggt tcaaaaaggc
     4261 atttgagtct gagccaacac tacagtcagg aattaattat gcagttctct tgctggcggc
     4321 tgggcaccag tttgaatctt cttttgagct tcggaaagtt ggagtgaagc tgagtagcct
     4381 tctgggtaaa aaggggaact tggaaaaact ccagagctac tgggaagtcg gattttttct
     4441 gggagccagt gtcctggcca acgaccacct cagggtcatt caggcatccg agaagctgtt
     4501 cagactgaag acaccagcgt ggtacctcaa gtctatcgtg gagaccattc tgatatataa
     4561 acactttgtg aaactgacca cagagcagcc ctcggctaag caggaactcg tggacttctg
     4621 gatggatttc ctggtcgagg ccacaaaaac agatgttact gttgtcaggt tcccagtatt
     4681 aatattagag ccaaccaaaa tctatcagcc ttcttacctg tctatcaata atgaagttga
     4741 ggagaagaca atatctattt ggcatgttct ccctgatgac aagaaaggta tacacgagtg
     4801 gaacttcggt gcttcctctg tcaggggagt gagcatctct aagtttgaag aacgatgctg
     4861 tttcctttat gtgcttcaca attctgatga cttccaaatc tacttctgca cagaacttca
     4921 ctgtaaaagg ttttttgaaa tggtgaatac catcacggaa gagaagggga gaggcgcgga
     4981 ggacggagac tgtgagggtg actccctgga gtatgactac gaatacgatg aaaatggaga
     5041 cagagttgtg ttagggaagg gcacttatgg gatcgtctat gcaggacgag acctgagcaa
     5101 ccaggtcaga attgctatta aggaaatccc agagagagac agcagatact ctcagcccct
     5161 gcatgaagaa atcgccctgc acaagcatct gaaacacaaa aacattgtcc agtatctggg
     5221 ctccttcagt gagaacggct tcatcaaaat cttcatggag caagtcccag gaggaagcct
     5281 ctctgctctc cttcgttcaa aatggggccc attaaaggac aacgaacaga cgattggctt
     5341 ctatacgaag cagatactgg aaggattaaa ataccttcac gacaatcaga tagtccaccg
     5401 ggatataaag ggtgacaatg tattgattaa tacctacagt ggtgtgctca agatctctga
     5461 cttcgggaca tccaagaggc ttgctggcat aaacccatgt accgaaacct ttacaggtac
     5521 ccttcagtat atggcgccag aaataatcga taaaggacca cgaggctatg gaaaggccgc
     5581 cgacatttgg tcactgggct gcacaatcat cgaaatggct actggaaaac caccatttta
     5641 tgaactagga gagccacagg cagccatgtt caaggtgggg atgtttaagg tccacccgga
     5701 gatcccagag tccatgtcgg cggaggccaa ggcgttcata ctgaagtgtt ttgaaccaga
     5761 ccctgacaag agagcctgtg ctaatgactt gcttattgat gagttcttaa aagtgtccag
     5821 caaaaagaaa aagacacaac ccaaactttc agctctctcg actggatcga atgagtatct
     5881 tagaagcatc tccctgccgg tccctgtcct ggtagaagac accagcagca gtagcgagta
     5941 tggctctgtt tctcctgata cagagctgaa ggcggacccc ttctccttca aagccagagc
     6001 taagtcctgt ggagaaaagg acgggaaagg gatacggaca ttgttcctgg gcattccaga
     6061 tgaaaatttt gaagatcaca gtgcgccccc atctcctgaa gagaaagact cgggcttttt
     6121 tatgctgaga aaggatagtg agaggcgagc tacccttcac agaatcctga ccgaagacca
     6181 ggacaaggtt gtaaggaacc taatggaatc tctggcccag ggtgctgaag agcctaaact
     6241 aaagtgggaa cacatcacaa ccctcatctc aagcctcaga gagtttgtga ggtccactga
     6301 ccgaaaaatc atagccacta cattgtccaa gctcaagctg gagctggact tcgacagcca
     6361 cggcatcagc caggtgcagg tggtcctctt tggcttccag gatgcggtca ataaagttct
     6421 tcggaatcat aacatcaagc cacactggat gtttgccctg gacagcatca tccgaaaggc
     6481 tgtgcagact gcaattacca ttctggttcc agagttgagg ccacatttta gccttgcatc
     6541 agagagcgac acagctgatc cggaagacct ggatgtcgaa gatgaacacg aggaattgtc
     6601 ttcaaatcag acagtccgac ggcctcaggc catcactgag gacgctgtgg ctacctcggg
     6661 ggtgagcacg ctaagctcca ctgtgtccca tgactcccag aatgcacacc ggtcgctgaa
     6721 cgtgcagctt ggaaggatga agattgaaac taataggtta cttgaagagc tggttcggaa
     6781 agagagagag ttacaggcac tcctccatca agccattgag gaaaaagacc aagaaattag
     6841 gcacctgaag ctcaagtccc agcccataga tatccctggg tttcctgtgt gccacctgaa
     6901 ctctcctggc acgaccacgg aagattctga acttcctggc tggctgagag aaaatggagc
     6961 tgatgaagac actataagtc ggtttctggc tgaggattac acgctagtgg atgttcttta
     7021 ctacgtcaca cgtgatgatc tgaagtgcct cagactaagg ggcgggatgc tgtgcacgct
     7081 gtggaaggcc atcattgact ttcggaacaa atgctaagga tcagcctcga ctgtgccttc
     7141 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     7201 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     7261 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     7321 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagcca
     7381 tcacaaagct ctgacctcaa tcctatagaa aggaggaatg agccaaaatt cacccaactt
     7441 attgtgggaa gcttgtggaa ggctactcga aatgtttgac ccaagttaaa caatttaaag
     7501 gcaatgctac caaatactaa ttgagtgtat gtaaacttct gacccactgg gaatgtgatg
     7561 aaagaaataa aagctgaaat gaatcattct ctctactatt attctgatat ttcacattct
     7621 taaaataaag tggtgatcct aactgaccta agacagggaa tttttactag gattaaatgt
     7681 caggaattgt gaaaaagtga gtttaaatgt atttggctaa ggtgtatgta aacttccgac
     7741 ttcaactgta tagggatcct ctagctagag tcgacctcga gggggggccc ggtacccagc
     7801 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     7861 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7921 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7981 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8041 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8101 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8161 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8221 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8281 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8341 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8401 tacctgtccg cctttctccc ttcgggaacc gtggcgcttt ctcatagctc acgctgtagg
     8461 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8521 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8581 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8641 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8701 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8761 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8821 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8881 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8941 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9001 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9061 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9121 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9181 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9241 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9301 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9361 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9421 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaactt ggccgcagtg
     9481 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9541 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9601 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9661 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9721 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9781 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9841 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9901 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9961 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
    10021 ttaagcgcgg cgggagtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    10081 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    10141 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    10201 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    10261 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    10321 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    10381 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    10441 ttaacgctta caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    10501 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    10561 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
    10621 cgtaatacga ctcactatag ggcgaattgg agctcggatc cctatacagt tgaagtcgga
    10681 agtttacata cacttaagtt ggagtcatta aaactcgttt ttcaactact ccacaaattt
    10741 cttgttaaca aacaatagtt ttggcaagtc agttaggaca tctactttgt gcatgacaca
    10801 agtcattttt ccaacaattg tttacagaca gattatttca cttataattc actgtatcac
    10861 aattccagtg ggtcagaagt ttacatacac taagttgact gtgcctttaa acagcttgga
    10921 aaattccaga aaatgatgtc atggctttag aagctagatc tggccatcta gagcggccgc
    10981 gcgcactagt gaattccatg gatatcaagc ttatcgatac cgtcgacctc
//
