LOCUS       sequence-440956-        8834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  METTL1-WT gene expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: pdest N-FLAG METTL1-WT puro
FEATURES             Location/Qualifiers
     source          1..8834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    229..250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(265..270,271..288,289..295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     327..343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     362..382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        364..382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        410..636
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             637..817
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    864..989
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1482..1715
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1900..1944
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2211..2327
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2350..2653
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2654..2857
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2808..2828
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2854..2878
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2962..2986
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2995..3018
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(3848..3872)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3903..4491
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3956..3976)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4374..4385)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4505..5004
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4540..4563)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4937..4956
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5025..5624
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5025..5044)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5521..5541
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5762..5995
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6067..6188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6104..6123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6158..6177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6228..6363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6383..6402)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6384..6402)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6412..6429)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6412..6428)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6421..6443)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6570..7025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6657..6676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6867..6888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7051..7155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7156..7224,7225..8016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7374..7393)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8187..8775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8676..8695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
      361 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
      421 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
      481 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
      541 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
      601 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
      661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      781 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      841 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      901 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      961 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1021 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1081 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1141 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1201 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1261 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1321 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1381 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1441 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1501 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1561 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1621 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1681 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1741 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1801 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1861 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1921 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1981 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2041 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2101 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2161 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga
     2221 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2281 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2341 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2401 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2461 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2521 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2581 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2641 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2701 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2761 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2821 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2881 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agtccagtgt
     2941 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggcttcac catggactac
     3001 aaagacgatg acgacaaggc agccgagact cggaacgtgg ccggagcaga ggccccaccg
     3061 ccccagaagc gctactaccg gcaacgtgct cactccaacc ccatggcgga ccacacgctg
     3121 cgctaccctg tgaagccaga ggagatggac tggtctgagc tatacccaga gttcttcgct
     3181 ccactcactc aaaatcagag ccacgacgat ccgaaagaca aaaaggaaaa gagagctcag
     3241 gcccaagtgg agtttgcaga cataggctgt ggctatggtg gcctgttagt ggaactgtca
     3301 ccgctgttcc cagacacact tattctgggt ctggagatcc gtgttaaagt gtctgattac
     3361 gtacaagacc ggattcgggc cctacgcgca gctcctgcag gtggcttcca gaacatcgcc
     3421 tgtctccgta gcaatgccat gaagcacctg ccgaattttt tttataaagg ccagctgaca
     3481 aagatgttct tcctcttccc cgacccgcac tttaaacgta cgaaacacaa gtggcgaatc
     3541 atcagtccca ccctgctagc agaatatgcc tacgtgctaa gagttggggg gctggtgtat
     3601 accataaccg atgtgctgga gctacacgat tggatgtgta cccactttga agagcaccca
     3661 ctgtttgagc gtgtgcctct ggaggacctg agtgaagacc ccgttgtggg acatctaggc
     3721 acctcaactg aggaggggaa gaaagttcta cgtaatggag ggaagaattt cccagccatc
     3781 ttccgaagaa tacaagatcc cgtcctccag gcagtgacct cccaaaccag cctgcctggt
     3841 cactgacacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
     3901 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3961 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4021 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4081 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4141 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4201 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4261 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     4321 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     4381 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4441 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     4501 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     4561 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     4621 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     4681 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     4741 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     4801 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     4861 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     4921 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     4981 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
     5041 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     5101 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     5161 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     5221 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     5281 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     5341 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     5401 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     5461 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     5521 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     5581 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     5641 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta
     5701 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5761 ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5821 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5881 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5941 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     6001 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6061 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6181 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     6241 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6301 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6361 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     6421 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6481 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6541 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     6601 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6661 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6721 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6781 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6841 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6901 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6961 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     7021 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7081 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7141 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7201 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7261 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7321 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7381 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7441 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7501 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7561 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7621 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7681 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7741 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7801 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7861 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7921 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7981 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8041 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8101 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8161 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8221 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8281 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8341 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8401 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8461 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8521 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8581 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8641 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8701 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8761 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8821 ccttttgctc acat
//
