LOCUS       sequence-441127-       11776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EF1-alpha-driven expression of RfxCas13d in mammalian cells. For 
            cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' 
            direct repeat. Clone using BsmBI. (Adapted from plasmid #138147).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11776)
  TITLE     LncRNA CISTR-ACT regulates cell size in human and mouse
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE...
FEATURES             Location/Qualifiers
     source          1..11776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..37)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(435..446)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             624..857
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(883..900)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    889..1113
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1159..1587
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1246..1265)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1456..1477
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1596..1616)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1601..1930
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1781..1916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1843..1862
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1978..2025
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2044..2418
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2548..2669
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2585..2604)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2639..2658
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2718..2734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2718..2734)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2731..2753)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2742..2758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2766..2772,2773..2790,2791..2796))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2811..2832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2949..2966)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3120..3708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3200..3219)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3879..4670,4671..4739))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4502..4521
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4740..4844)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4919..4938)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5110..5489
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5491..5689
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5644..5664
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5707..5887
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5934..6059
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6552..6785
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6970..7014
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7312..7429
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7480..7728
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7480..7500
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7651..7670
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7730..7765
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        7782..7817
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     promoter        7880..8091
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             8116..11013
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="MIEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDS
                     IRSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLE
                     KRYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTV
                     YTYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYII
                     NYGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITN
                     ELTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRK
                     DMSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFV
                     INLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPA
                     GRDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAF
                     FKDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDE
                     NGNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQ
                     KKQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGRE
                     NAEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLE
                     EKGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKK
                     AEEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVH
                     AYINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCV
                     PFGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGN"
     CDS             11014..11061
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             11071..11127
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             11128..11724
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     11621..11641
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(11740..11776,1..552)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
ORIGIN
        1 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
       61 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
      121 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
      181 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
      241 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
      301 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
      361 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
      421 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
      481 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
      541 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
      601 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
      661 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      721 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      781 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
      841 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
      901 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
      961 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     1021 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     1081 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     1141 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     1201 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     1261 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     1321 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     1381 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     1441 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     1501 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     1561 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     1621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1681 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     1741 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     1801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     1861 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     1921 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     1981 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     2041 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     2101 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     2161 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     2221 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     2281 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     2341 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     2401 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     2461 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     2521 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     2581 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2641 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     2701 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2761 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2821 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2881 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2941 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3001 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3061 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3121 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3181 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3241 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3301 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3361 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3421 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3481 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3541 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3601 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3661 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3721 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3781 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3841 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3901 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3961 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4021 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4081 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4141 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4201 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4261 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4321 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4381 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4441 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4501 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4561 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4621 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4681 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4741 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4801 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4861 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     4921 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     4981 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     5041 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     5101 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     5161 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     5221 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     5281 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     5341 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     5401 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     5461 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     5521 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     5581 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     5641 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc
     5701 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5761 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5821 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5881 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     5941 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     6001 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     6061 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     6121 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     6181 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     6241 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     6301 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     6361 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     6421 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     6481 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     6541 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     6601 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     6661 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     6721 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     6781 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     6841 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     6901 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     6961 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     7021 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     7081 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     7141 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     7201 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     7261 tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag
     7321 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     7381 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     7441 cagggacagc agagatccag tttggttaat taaggtaccg agggcctatt tcccatgatt
     7501 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
     7561 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
     7621 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
     7681 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc aagtaaaccc
     7741 ctaccaactg gtcggggttt gaaacggaga cggacgtctc tcaagtaaac ccctaccaac
     7801 tggtcggggt ttgaaacttt ttttgaattc gctagctagg tcttgaaagg agtgggaatt
     7861 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     7921 ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     7981 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     8041 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggttc
     8101 tagagcgctg ccaccatgat cgaaaaaaaa aagtccttcg ccaagggcat gggcgtgaag
     8161 tccacactcg tgtccggctc caaagtgtac atgacaacct tcgccgaagg cagcgacgcc
     8221 aggctggaaa agatcgtgga gggcgacagc atcaggagcg tgaatgaggg cgaggccttc
     8281 agcgctgaaa tggccgataa aaacgccggc tataagatcg gcaacgccaa attcagccat
     8341 cctaagggct acgccgtggt ggctaacaac cctctgtata caggacccgt ccagcaggat
     8401 atgctcggcc tgaaggaaac tctggaaaag aggtacttcg gcgagagcgc tgatggcaat
     8461 gacaatattt gtatccaggt gatccataac atcctggaca ttgaaaaaat cctcgccgaa
     8521 tacattacca acgccgccta cgccgtcaac aatatctccg gcctggataa ggacattatt
     8581 ggattcggca agttctccac agtgtatacc tacgacgaat tcaaagaccc cgagcaccat
     8641 agggccgctt tcaacaataa cgataagctc atcaacgcca tcaaggccca gtatgacgag
     8701 ttcgacaact tcctcgataa ccccagactc ggctatttcg gccaggcctt tttcagcaag
     8761 gagggcagaa attacatcat caattacggc aacgaatgct atgacattct ggccctcctg
     8821 agcggactga ggcactgggt ggtccataac aacgaagaag agtccaggat ctccaggacc
     8881 tggctctaca acctcgataa gaacctcgac aacgaataca tctccaccct caactacctc
     8941 tacgacagga tcaccaatga gctgaccaac tccttctcca agaactccgc cgccaacgtg
     9001 aactatattg ccgaaactct gggaatcaac cctgccgaat tcgccgaaca atatttcaga
     9061 ttcagcatta tgaaagagca gaaaaacctc ggattcaata tcaccaagct cagggaagtg
     9121 atgctggaca ggaaggatat gtccgagatc aggaaaaatc ataaggtgtt cgactccatc
     9181 aggaccaagg tctacaccat gatggacttt gtgatttata ggtattacat cgaagaggat
     9241 gccaaggtgg ctgccgccaa taagtccctc cccgataatg agaagtccct gagcgagaag
     9301 gatatctttg tgattaacct gaggggctcc ttcaacgacg accagaagga tgccctctac
     9361 tacgatgaag ctaatagaat ttggagaaag ctcgaaaata tcatgcacaa catcaaggaa
     9421 tttaggggaa acaagacaag agagtataag aagaaggacg cccctagact gcccagaatc
     9481 ctgcccgctg gccgtgatgt ttccgccttc agcaaactca tgtatgccct gaccatgttc
     9541 ctggatggca aggagatcaa cgacctcctg accaccctga ttaataaatt cgataacatc
     9601 cagagcttcc tgaaggtgat gcctctcatc ggagtcaacg ctaagttcgt ggaggaatac
     9661 gcctttttca aagactccgc caagatcgcc gatgagctga ggctgatcaa gtccttcgct
     9721 agaatgggag aacctattgc cgatgccagg agggccatgt atatcgacgc catccgtatt
     9781 ttaggaacca acctgtccta tgatgagctc aaggccctcg ccgacacctt ttccctggac
     9841 gagaacggaa acaagctcaa gaaaggcaag cacggcatga gaaatttcat tattaataac
     9901 gtgatcagca ataaaaggtt ccactacctg atcagatacg gtgatcctgc ccacctccat
     9961 gagatcgcca aaaacgaggc cgtggtgaag ttcgtgctcg gcaggatcgc tgacatccag
    10021 aaaaaacagg gccagaacgg caagaaccag atcgacaggt actacgaaac ttgtatcgga
    10081 aaggataagg gcaagagcgt gagcgaaaag gtggacgctc tcacaaagat catcaccgga
    10141 atgaactacg accaattcga caagaaaagg agcgtcattg aggacaccgg cagggaaaac
    10201 gccgagaggg agaagtttaa aaagatcatc agcctgtacc tcaccgtgat ctaccacatc
    10261 ctcaagaata ttgtcaatat caacgccagg tacgtcatcg gattccattg cgtcgagcgt
    10321 gatgctcaac tgtacaagga gaaaggctac gacatcaatc tcaagaaact ggaagagaag
    10381 ggattcagct ccgtcaccaa gctctgcgct ggcattgatg aaactgcccc cgataagaga
    10441 aaggacgtgg aaaaggagat ggctgaaaga gccaaggaga gcattgacag cctcgagagc
    10501 gccaacccca agctgtatgc caattacatc aaatacagcg acgagaagaa agccgaggag
    10561 ttcaccaggc agattaacag ggagaaggcc aaaaccgccc tgaacgccta cctgaggaac
    10621 accaagtgga atgtgatcat cagggaggac ctcctgagaa ttgacaacaa gacatgtacc
    10681 ctgttcagaa acaaggccgt ccacctggaa gtggccaggt atgtccacgc ctatatcaac
    10741 gacattgccg aggtcaattc ctacttccaa ctgtaccatt acatcatgca gagaattatc
    10801 atgaatgaga ggtacgagaa aagcagcgga aaggtgtccg agtacttcga cgctgtgaat
    10861 gacgagaaga agtacaacga taggctcctg aaactgctgt gtgtgccttt cggctactgt
    10921 atccccaggt ttaagaacct gagcatcgag gccctgttcg ataggaacga ggccgccaag
    10981 ttcgacaagg agaaaaagaa ggtgtccggc aataagcgac ctgccgccac aaagaaggct
    11041 ggacaggcta agaagaagaa aggatccggc gcaacaaact tctctctgct gaaacaagcc
    11101 ggagatgtcg aagagaatcc tggaccgacc gagtacaagc ccacggtgcg cctcgccacc
    11161 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
    11221 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
    11281 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
    11341 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
    11401 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
    11461 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc
    11521 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
    11581 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
    11641 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
    11701 atgacccgca agcccggtgc ctgaacgcgt taagtcgaca atcaacctct ggattacaaa
    11761 atttgtgaaa gattga
//
