LOCUS       sequence-44141-e        3666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: Wnt5B STOP
FEATURES             Location/Qualifiers
     source          1..3666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1791..1890)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1907..1926)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1908..1926)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1931..1947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1931..1947)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2049..2068)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2060..2869
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2127..2146)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3016..3604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3505..3524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccat gcccagcctg ctgctgctgt tcacggctgc
      721 tctgctgtcc agctgggctc agcttctgac agacgccaac tcctggtggt cattagcttt
      781 gaacccggtg cagagacccg agatgtttat catcggtgcc cagcccgtgt gcagtcagct
      841 tcccgggctc tcccctggcc agaggaagct gtgccaattg taccaggagc acatggccta
      901 cataggggag ggagccaaga ctggcatcaa ggaatgccag caccagttcc ggcagcggcg
      961 gtggaattgc agcacagcgg acaacgcatc tgtctttggg agagtcatgc agataggcag
     1021 ccgagagacc gccttcaccc acgcggtgag cgccgcgggc gtggtcaacg ccatcagccg
     1081 ggcctgccgc gagggcgagc tctccacctg cggctgcagc cggacggcgc ggcccaagga
     1141 cctgccccgg gactggctgt ggggcggctg tggggacaac gtggagtacg gctaccgctt
     1201 cgccaaggag tttgtggatg cccgggagcg agagaagaac tttgccaaag gatcagagga
     1261 gcagggccgg gtgctcatga acctgcaaaa caacgaggcc ggtcgcaggg ctgtgtataa
     1321 gatggcagac gtagcctgca aatgccacgg cgtctcgggg tcctgcagcc tcaagacctg
     1381 ctggctgcag ctggccgagt tccgcaaggt cggggaccgg ctgaaggaga agtacgacag
     1441 cgcggccgcc atgcgcgtca cccgcaaggg ccggctggag ctggtcaaca gccgcttcac
     1501 ccagcccacc ccggaggacc tggtctatgt ggaccccagc cccgactact gcctgcgcaa
     1561 cgagagcacg ggctccctgg gcacgcaggg ccgcctctgc aacaagacct cggagggcat
     1621 ggatggctgt gagctcatgt gctgcgggcg tggctacaac cagttcaaga gcgtgcaggt
     1681 ggagcgctgc cactgcaagt tccactggtg ctgcttcgtc aggtgtaaga agtgcacgga
     1741 gatcgtggac cagtacatct gtaaatgaat tctgaagggt gggcgcgccg acccagcttt
     1801 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     1861 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     1921 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     1981 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     2041 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     2101 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     2161 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     2221 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     2281 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     2341 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     2401 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     2461 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     2521 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     2581 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     2641 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     2701 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     2761 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     2821 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     2881 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     2941 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     3001 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3061 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3121 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc
     3421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3661 catgtt
//
