LOCUS       sequence-441458-        9241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression AtRBX1-T7,  AtASK1-T7 and AtCUL1-S in in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Pu ZX, Wang JL, Li YY, Liang LY, Tan YT, Wang ZH, Li BL, Guo GQ, 
            Wang L, Wu L
  TITLE     A Bacterial Platform for Studying Ubiquitination Cascades Anchored 
            by SCF-Type E3 Ubiquitin Ligases.
  JOURNAL   Biomolecules. 2024 Sep 25;14(10):1209. doi: 10.3390/biom14101209.
  PUBMED    39456142
REFERENCE   2  (bases 1 to 9241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomolecules."; date: "2024-09-25"; volume: "14(10):1209. doi"; 
            pages: " 10.3390/biom14101209"
COMMENT     Sequence Label: pETDuet-AtRBX1-T7-AtASK1-T7-AtCUL1-S
FEATURES             Location/Qualifiers
     source          1..9241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     206..225
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        313..331
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    332..356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             371..393
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             844..876
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             2037..2069
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        2147..2165
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2166..2190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4519..4563
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(4601..4619)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4615..4662
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4699..5154
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4786..4805)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4996..5017
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5269..6060,6061..6129))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5892..5911
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6130..6222)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6303..6891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6792..6811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7045..7062
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7077..7216
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7142..7161)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7302..7324
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7318..7509)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8081..9163)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9125..9144
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9164..9241)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
       61 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
      121 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
      181 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
      241 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atcgatctcg
      301 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
      361 tagaaataat tttgtttaac tttaagaagg agatatacca tggcgactct agactccgac
      421 gttaccatga ttcctgccgg agaagcctcc agcagcgtag ccgcgtcgtc ttccaacaag
      481 aaagctaagc gattcgaaat taagaagtgg agcgccgttg ctctctgggc ttgggatatc
      541 gttgttgaca actgtgcgat ctgcagaaac cacatcatgg atctttgtat cgagtgtcag
      601 gctaatcagg ccagtgccac aagtgaagag tgcactgtag cttgggagga cgaccaaaat
      661 aactgcaata agtatttctg tattcttgat tgctcaatga aagatgacca cttggagggg
      721 gtttgcaatc acgccttcca ctttcactgc atcagcagat ggctaaagac tcgtcaagtt
      781 tgtccattgg ataacagtga gtgggagttt cagaaatatg gtcacagcca ggatccgaat
      841 tcgatggcta gcatgactgg tggacagcaa atgggtccat aaccccgaaa gaaggagata
      901 tacatatgga caagtacgag ctggtgaaag acataggtgc tgggaatttt ggagttgcca
      961 ggctcatgaa ggtcaaaaac tctaaggaac ttgttgccat gaagtacatc gagcgtggtc
     1021 ctaagattga tgagaatgtg gcaagagaga tcattaatca cagatcactt cgccatccga
     1081 atataatccg gttcaaggag gtggtgttga ctccaaccca tcttgccatt gctatggaat
     1141 atgctgctgg tggtgaacta ttcgagcgta tatgcagtgc tggaagattt agtgaggatg
     1201 aggaggaagg gaacaagaga aaacatgcga gatatttctt ccagcagctt atatcaggtg
     1261 ttagctattg ccatgctatg caaatatgcc atagagatct gaagctcgag aatacgctct
     1321 tggatggaag tcctgctcca cgtctgaaaa tctgtgattt tggttattcc aagtcctctc
     1381 tgctgcactc taggcccaaa tcaacagttg gaactccagc atatattgca cctgaggtcc
     1441 tttctcgaag agaatatgat ggcaagatgg ctgatgtatg gtcttgtggt gtgactcttt
     1501 atgtcatgct ggttggagca tacccatttg aagaccagga agaccccaag aacttcagga
     1561 aaacaataca aaaaataatg gctgtccagt acaagatccc ggactacgtc catatctcac
     1621 aggattgtaa aaatctcctt tcccgtatat ttgtcgccaa ttcactcaag aggatcacca
     1681 ttgcagaaat caagaaacat tcatggttcc taaagaattt gccaagggaa ctcacagaga
     1741 cagctcaagc tgcatatttc aagaaagaga acccaacctt ctcccttcag accgttgaag
     1801 agatcatgaa gatagtggct gacgccaaaa caccgcctcc tgtttcccga tccatcggag
     1861 gttttggctg gggaggaaat ggggatgcag atggaaaaga ggaagatgca gaagacgtgg
     1921 aggaggaaga ggaggaggtg gaagaagagg aagacgatga ggatgaatac gataagactg
     1981 taaaggaagt acacgcaagt ggagaagtga gaataagtag ctcggcgcgc ctgcagatgg
     2041 ctagcatgac tggtggacag caaatgggtg tcgacaagct tgcggccgca taatgcttaa
     2101 gtcgaacaga aagtaatcgt attgtacacg gccgcataat cgaaattaat acgactcact
     2161 ataggggaat tgtgagcgga taacaattcc ccatcttagt atattagtta agtataagaa
     2221 ggagatatac atatggcaga tctcaattgg atatcggccg gccacgcgat cgctgacgtc
     2281 ggtaccatgg agcgcaagac tattgacttg gagcaaggat gggactatat gcagactgga
     2341 attactaagc tgaaacggat tctggaagga ttgaatgagc cagcatttga ctctgagcaa
     2401 tatatgatgc tttatacgac tatctacaac atgtgcaccc agaaacctcc tcatgattac
     2461 tcacagcagc tttatgacaa gtatcgggaa gcatttgagg aatatattaa ctcaacagtt
     2521 ttgcctgcgt tgagggagaa gcatgatgag tttatgctgc gggagctatt taaaagatgg
     2581 tcaaaccata aagtaatggt cagatggcta tcccgcttct tctactacct tgaccgttac
     2641 ttcattgctc ggagatcact tccaccactg aatgaagttg gcctgacatg cttccgtgac
     2701 ctggtttata atgagctaca ttctaaggtc aaacaagctg taatagctct tgttgataaa
     2761 gaacgggagg gcgagcagat tgatagggcc ctgctgaaaa acgtattaga tatctatgta
     2821 gagattggaa tggggcagat ggagaggtat gaagaagatt ttgaaagctt catgcttcaa
     2881 gatacttctt cgtattattc tcgcaaggca tcaagctgga ttcaggaaga ttcttgccct
     2941 gattacatgt tgaagtctga agaatgtcta aagaaggaga gggagagagt ggctcactac
     3001 ctacactcaa gcagtgagcc aaagctggtt gagaaagtac aacatgaatt gctggttgtg
     3061 tttgcaagtc agcttctaga aaaagaacac tcagggtgcc gtgcattgct aagagatgac
     3121 aaggtggatg atctctccag gatgtacagg ctttaccata aaattttgcg aggcttggaa
     3181 cctgttgcaa acatctttaa gcagcatgtc acagcagagg gtaacgctct tgtccaacag
     3241 gccgaagaca cggctactaa tcaggttgca aatactgcta gcgtccagga acaggttctt
     3301 atcagaaaag tgattgaact tcatgataaa tacatggtat atgtcaccga gtgtttccag
     3361 aaccacaccc tcttccataa ggctttgaaa gaggcatttg agattttttg taacaaaacg
     3421 gttgctggaa gttcaagtgc agaactactt gcaacatttt gcgacaatat tctcaaaaag
     3481 gggggaagtg aaaagctgag tgatgaagct atcgaagata cgcttgagaa ggttgtcaaa
     3541 ttgcttgcat acataagtga caaggatctt ttcgctgagt tctacaggaa gaagctggcc
     3601 cgtaggctct tatttgatcg cagtgctaat gatgatcatg agagaagtat cctgacaaag
     3661 ctcaagcaac aatgtggtgg acagtttact tctaagatgg agggcatggt gacggatttg
     3721 acactggcaa gagaaaacca aaacagtttc gaggattatc taggcagtaa ccctgctgca
     3781 aacccaggga ttgacttgac cgtcactgtt cttaccactg gtttctggcc aagttacaaa
     3841 tcatttgaca taaatctacc cagtgaaatg atcaagtgtg ttgaagtctt caaagggttt
     3901 tatgaaacga aaacgaaaca caggaagctt acgtggatct attcactggg aacttgtcac
     3961 ataaacggga agtttgatca aaaggccatc gagttaatag tgtctactta ccaggctgct
     4021 gtgcttctac tctttaacac aactgacaag ttaagttaca ctgagatctt ggctcaactg
     4081 aacctaagcc atgaagatct agttaggttg cttcattcct tgtcatgtgc taagtacaag
     4141 atactcctta aggagccaaa caccaagact gtctcccaga atgatgcctt tgagttcaac
     4201 tccaaattca ccgatagaat gcgcagaatc aagatccctc ttcccccagt tgatgaaagg
     4261 aagaaagtcg ttgaagatgt cgataaagac agaagatatg caattgatgc tgccattgtc
     4321 aggatcatga agagcaggaa agtattggga catcaacaac ttgtttctga gtgtgttgag
     4381 caacttagcc gaatgttcaa gcctgatatc aaagcgatca agaagcgtat ggaggattta
     4441 ataaccagag attatttgga gagggacaag gagaatccta acatgtttag gtacttggct
     4501 ggtaccctcg agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca gcacatggac
     4561 tcgtctacta gcgcagctta attaacctag gctgctgcca ccgctgagca ataactagca
     4621 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     4681 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4741 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4801 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4861 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4921 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4981 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5041 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5101 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttctggcg
     5161 gcacgatggc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5221 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5281 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5341 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5401 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5461 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5521 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5581 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5641 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5701 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5761 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5821 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5881 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5941 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6001 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6061 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6121 aatactcata ctcttccttt ttcaatcatg attgaagcat ttatcagggt tattgtctca
     6181 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggtcat gaccaaaatc
     6241 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6301 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6361 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6421 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6481 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6541 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6601 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6661 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6721 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6781 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6841 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6901 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6961 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7021 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     7081 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca
     7141 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     7201 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     7261 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     7321 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     7381 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     7441 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     7501 tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat
     7561 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     7621 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
     7681 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca
     7741 tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact
     7801 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     7861 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     7921 accccgccag cctagccggg tcctcaacga caggagcacg atcatgctag tcatgccccg
     7981 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
     8041 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8101 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8161 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
     8221 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
     8281 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
     8341 cgtcgtatcc cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc
     8401 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
     8461 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
     8521 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
     8581 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
     8641 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
     8701 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
     8761 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
     8821 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
     8881 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
     8941 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
     9001 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
     9061 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
     9121 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
     9181 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
     9241 c
//
