LOCUS       sequence-441661-        5670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  osm-10 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY113
FEATURES             Location/Qualifiers
     source          1..5670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1035..1055
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1077..1097
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3261..3283)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3383..3402
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3480..3498)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3566..3670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3671..3739,3740..4531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3889..3908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4702..5290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5191..5210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5444..5461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5578..5599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5614..5619,5620..5637,5638..5644)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5652..5668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5657..5670,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt gaattggatc
      181 acacaccgga agtggtatga cagtattcat taacagattc aatttgaaat attaatttta
      241 ggttagatgt gtattctgca aaagctcatt cgatcagcca ctggaaatga acgatgaaca
      301 atgggcacac tttttcgctc atatttatgg ggttagtaat tcaccattcg aatctagaag
      361 tcgattctcc tgactgacat ttttaaaacc attttttcca ggaaatcctc tactcccgcc
      421 ttgccgacta cgaggatcgt gctttgaaca ctgcatgaat tatatatttg attcgatggt
      481 tagcttaata aagaattttt ctgttgtact tcaacctttt tctgaaatag gaaataatca
      541 caagttgtct gaaaaatgtg atcagctatc cagatcaaac agtgaaaatg ttgtctgcaa
      601 tgattcatag ctgaacatct gaactcttaa attttcagcg aatcatgatt tgcattgtga
      661 tatattattc ttatggttgc aaaagaaaaa agattacgaa aatatcttgt gacagagtaa
      721 gaaaccattg aaatttaatt aatcaccgtg gagacaggtg tgcactctgc tcttattata
      781 ttatcaaaac gagaaacgcg tgagcaagaa gtgggcgtga cttggtagaa aggtacttaa
      841 tcgagatgtt gagccaactt tcgagattat tctaaaagtt tccatatcaa taaacttttt
      901 tggaaatttc atcttgcaag cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
      961 atactaacat aacatagaac attttcagga ggacccttgc ttggagggta ccgagctcag
     1021 aaaaaatgac cgctccaaag aagaagcgca aggttccagt cgagaagatg actgccccaa
     1081 aaaagaaacg taaggtcccg cggatgtcag agcttattaa ggagaatatg catatgaaac
     1141 tgtacatgga aggaaccgtg gacaaccatc actttaagtg cacttcggag ggggaaggca
     1201 aaccgtacga gggcacccag accatgcgaa tcaaggtggt cgagggaggc ccactgccct
     1261 tcgccttcga cattctggta agtttaaaca tatatatact aactaaccct gattatttaa
     1321 attttcaggc tactagcttc ctgtacggca gcaagacttt tattaatcac acccagggca
     1381 tcccggactt cttcaaacag tcgttccctg agggcttcac atgggagcgt gtgaccacat
     1441 acgaagacgg tggcgttctc accgctaccc aggacaccag cctccaggac ggttgcctca
     1501 tatataatgt ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     1561 agaagatccg gggagtaaac ttcacatcca atggtccggt tatgcagaag aaaacactcg
     1621 gctgggaggc cttcaccgag acgctgtatc cggctgacgg aggcctggaa ggtcgaaacg
     1681 acatggccct gaagctcgtc ggcggtagcc atctcatcgc aaacgctaag accacatata
     1741 gatccaagaa accggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1801 ttcaggctaa gaaccttaaa atgccgggag tctactatgt tgactacaga ctggaacgga
     1861 tcaaggaggc caacaacgag acctacgtcg agcagcacga ggttgcagtt gccagatact
     1921 gcgacctccc gagcaaactg ggtcacaagc ttaatcctag gataccggtg ggtgaagacc
     1981 caccaaagcc atctgccaag ggagccaaga aggccgccaa gaccgttacg aagccaaagg
     2041 acggaaagaa gagacgtcat gcccgtaagg aatcatactc cgtctacatc taccgtgtcc
     2101 tcaagcaagt tcatccagac actggagttt cctccaaagc catgtctatc atgaactctt
     2161 ttgtcaacga tgtcttcgag cgtattgctg ctgaagcatc ccgtcttgct cactacaaca
     2221 agcgttccac aatctcatcc cgcgaaattc agaccgctgt ccgtctgatc cttccaggag
     2281 agcttgccaa gcacgccgtg tctgagggaa ccaaggccgt taccaagtac acttccagca
     2341 agtaggaatt ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat
     2401 aataggggcc gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata
     2461 tttaaatttt cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac
     2521 atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac
     2581 ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt
     2641 agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc
     2701 cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt
     2761 ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc
     2821 atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca
     2881 tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca
     2941 agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt
     3001 gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg
     3061 caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat
     3121 aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca
     3181 atataccaaa cataactgtt tcctactagt cggccgtacg ggccctttcg tctcgcgcgt
     3241 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     3301 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     3361 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     3421 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg
     3481 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3541 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3601 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3661 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3721 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3781 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3841 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3901 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3961 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4021 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4081 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4141 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4201 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4261 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4321 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4381 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4441 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4501 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4561 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4621 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4681 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4741 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4801 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4861 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4921 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4981 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5041 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5101 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5161 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5221 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5281 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5341 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5401 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     5461 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     5521 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     5581 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5641 gttgtgtgga attgtgagcg gataacaatt
//
