LOCUS       sequence-441663-        5583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  oig-1 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY112
FEATURES             Location/Qualifiers
     source          1..5583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1258..1278
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1303..1323
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1345..1365
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3174..3196)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3296..3315
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3393..3411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3479..3583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3584..3652,3653..4444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3802..3821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4615..5203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5104..5123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5491..5512
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5527..5532,5533..5550,5551..5557)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5565..5581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5570..5583,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc cacatggctt
      181 aactctctcc catttttcga cgtttcgaga acctttcatt tcgtgtcagg gtctttttat
      241 tttgaaatcg aaaaaaaaca ctcttacttt tatgtcaatt gaaaaaaaag gcttggaaag
      301 gggcatagac gggaggaaaa ttgaatcgag tctccctcct gaagtccctt ttttgaaaaa
      361 tccgtttctt tcactctcct ttttagcttt tcaacaacct tttcaaccca ttcgtgacct
      421 gaaaacttta gtttttgcta attgtcattt tgttttcgag gcgacgtttt gatgctagga
      481 aaacaatttt caaagaagct gtgatgcgct taaataacgt ctattttcat aattttatga
      541 gttcctaact ttttcaaagg ttttatagca tttaaataat atgtttgctt tcgattctga
      601 tccaaaaata aaacctaacc tattgtagga attttatcta gaacttgcat cactgttttt
      661 atctgaacta atcgaaacct aaccattcca aaagattaat cccaaaacga tcacatcatt
      721 tcaaaggaga gccttccttg ctgtgctatt gtgctctccg tgttacgcct cattactcaa
      781 ttttttgacc tctttcctaa tgaaatcggc ttcaaagctc agagctcgca gaacaagagg
      841 aacgttgaga aaaaggaatt ttttctaaaa atcacgtgtt tcttgtgctt ccggatttag
      901 gggttccttt aagcagaaaa atggtattaa aaatgtatct taaaagtttt caatcacaaa
      961 gaaattgaat taaactcctt acaattttga acttttcatg ttaacgcaca atctttattt
     1021 ccgtcattgt tgaatgtttt ttactcatat aaatttccaa gtatattaac atattcattc
     1081 cagttctcat tatgacataa ttctgcaaaa cgttcgactc ccgggattgg ccaaaggacc
     1141 caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag gacccttgga
     1201 gggtaccggt tttcccagtc acgacgcgtg gtaccgagct cagaaaaaat gaccgctcca
     1261 aagaagaaac gcaaagtacc gagctcagaa aaaatgacgg cacctaaaaa gaaacgcaaa
     1321 gtaccggtcg ccgagaagac agccccaaaa aagaagcgta aagttccacg cgtcatggga
     1381 gtaagtttaa acagttcggt actaactaac catacatatt taaattttca gcgcgctgtc
     1441 tccaagggag aggagctcat caaggagaac atgcacacca agctctacat ggagggaacc
     1501 gtcaacaacc accacttcaa gtgcacccac gaaggggagg gcaagcccta cgagggcacc
     1561 cagaccaaca ggattaaggt ggtggaggga ggccccctgc cgttcgcatt cgacatcctg
     1621 gccacctgct ttatgtacgg gagcaagacc ttcatcaacc acacccaagg aatcccagac
     1681 ttcttcaagc aatccttcgt aagtttaaac atgattttac taactaacta atctgattta
     1741 aattttcagc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
     1801 cttaccgtta cccaggacac cagcctccag gacggctgct tgatctacaa cgtcaagctc
     1861 agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag
     1921 gccagtaccg agaccctgta ccccgctgac ggcggcctgg aaggcagatg cgacatggcc
     1981 ctgaagctcg tgggcggggg ccacctgcac tgcaacctga agaccacata cagatccaag
     2041 aaacccgcta agaacctcaa gatgccagga gtaagtttaa acatatatat actaactaac
     2101 cctgattatt taaattttca ggtctacttt gtggaccgca gactggaaag aatcaaggag
     2161 gccgacaatg agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc
     2221 cctagcaaac tggggcacaa acttaatggc atggacgagc tgtacaagta agcgccggtc
     2281 gctaccatta ccaacttgtc tggtgtcaaa aataataggg gccgctgtca tcagagtaag
     2341 tttaaactga gttctactaa ctaacgagta atatttaaat tttcagcatc tcgcgcccgt
     2401 gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc
     2461 tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct
     2521 tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa
     2581 taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc
     2641 tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca
     2701 tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc
     2761 aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa
     2821 aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc
     2881 tgcttttgct ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc
     2941 ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt
     3001 ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg
     3061 ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact
     3121 agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     3181 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     3241 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     3301 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     3361 aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt
     3421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4741 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5221 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5341 ccgaacgacc gagcgcagcg agtcagtgag cgatgaagcg gaagagcgcc caatacgcaa
     5401 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     5461 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     5521 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     5581 att
//
