LOCUS       sequence-441671-        7336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sra-11(pB) fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY120
FEATURES             Location/Qualifiers
     source          1..7336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3011..3031
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3056..3076
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3098..3118
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4927..4949)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5049..5068
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5146..5164)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5232..5336
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5337..5405,5406..6197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5555..5574)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6368..6956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6857..6876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7244..7265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7280..7285,7286..7303,7304..7310)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7318..7334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7323..7336,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt taatcaatgt
      181 gtttgctccg atgcgcctag tctcaaagcc ttctcatgtt gcaaacaaat gcactaacta
      241 aactaattaa caagtcacgg aggagattca aaacatttca cttttcagga aagtagcatt
      301 tttaaatata ttcatgttgt agatttgtca ttttgcaata ttttttatca aaattggatt
      361 acaccctctt ttgagcagtc tgacttgttg gctgacttga ttttctttta aagtgatgaa
      421 attttattca tatttgattt ttagtaaata aattattgca aaccccatta tgcaactttg
      481 acagcttata tctcagttgc cgtttgtttt atgaaaaatt gtcaacttac caaataattc
      541 ttagttacga tctgaaattt ttcgaaaact ctaatcttta gtcaaaaatg ttcagcaatg
      601 taaacatttt ttcattacaa aaaaagatga tgtagatgca tacatcaagt atctcaaccg
      661 gaacaccccc tattttgcac catctctgtc atctttttct tcctttttca gatgggaaac
      721 atacaattaa tgctcacatg tcatgtctag tcatttgttt ttttttgttt gtttttaatc
      781 cgaatctctt attcatcttt agacgttcca ttcggcagag gcgagttggg gcgcctctta
      841 ctcgcctcaa attttgtgtt tgcactcata atcaaagaat aaattatctt gcccattttg
      901 gtccttgacc atttgtgtag gcaataggca ggcagacata ttttcttaaa cagaagaccg
      961 aactgtgagt gtacaaaaat aaattaaata ttacctacct ttgttagaat ttggaataag
     1021 attaaaaaat tcgttgtcct ctgtggaatt gctcattatt agatttttac gaaatcatgc
     1081 ggattagaaa gtttaatatt tatgtacagt gcgtccaact tctatagcac ccccctccaa
     1141 ttcggccgtt tggagctttt caaaatattt ttcggaaaag tgttagtgca gttcgatagc
     1201 aaatattgaa aaacctatgc tccccaattt ttatcatgat atctcaaaaa tcacggaaat
     1261 tgggtcaaaa tatctgaaaa atggccgtcg aacttctata gcaccccctc tgatttttga
     1321 caaatttctg taaattcggg gttttcttcg tagttaatga atttattttg taaataactg
     1381 cacaccaaaa taatcaggtg gtttcacttt cacatttaaa aaataaatat aacacttgaa
     1441 aacacgtgga atgcctggga ttgatcaaaa aattgatcaa aatgttcttc tccgtctatt
     1501 cattactatg taattagttg tagaaagcat ttattcatat gaaccaaatg gtaaagggag
     1561 caacttcagt ggtttttaaa aggctcgatt taaccatgaa aacgagcgtt cggaggcaca
     1621 aagctgaagg ttttgacttc gtattgtaca gaacttcagc tcatcctgct caaatgtctt
     1681 tgaagcttcg attttgatta gatgtctttg aaaatttata cggaaaagat catgagaccg
     1741 ctcaaaaaga ggacaaaggc aaagcgaggc cgcaagtctt tttttcaagc taacatgcga
     1801 aaattttaga cagaaaagta agatatacct ttccaaatga cgattttgct agttgtattt
     1861 ataactcaag ttaaatttag cgaattcgct cattgaatct ttttttggtc aagtaatgca
     1921 aaagctacca gacaaaacat tgaacctgcg attgtagatg tctggcatct gatttaccca
     1981 tggatttcca cggatttttt gttcatataa aataattttt aatttttgct ataatgtagt
     2041 aatttttagt cgaaaatgta ttcccaacca ttatctctgc gcctccgaac gctcgttttc
     2101 atggttaaat cgaacctttt aaaaaccact gaagttgctc cctttaccat ttggttcata
     2161 tgaataaatg ctttctacaa ctaattatat agtaatgaat agacggagaa gaacattttg
     2221 atctattttg gtgctgcgaa aatcattggg agccaagata tggcctctga agcaaataag
     2281 aacgttgagg ggggtgctat agaagttgga cgcactgtat ttccaattat tttttaagaa
     2341 tttcgatttt ataaacagcc tttgctccgc ctatttgagc tcatgatgtt tctcttttgt
     2401 tttcaagatt gttgattcct tcctattgtc ctattgtgat ttttaaattt ttaaattgct
     2461 cattaacttg atttttattt agtacatatc tctaatatgt tgtttcacat tgcaccaacc
     2521 ggtctcaatt tttgaaatta tttattgtta tgtttgaata ttttaaacaa atttaaaaat
     2581 ttagaaaaat tgctgagctg ttgcctaaac tcataagtgt ttcaaaattt gctacgattt
     2641 tgcatgtact ttttaatatt ttatatgctt tttcgtattc caaaacaaac aatttagttt
     2701 actttacaaa ttatgaacaa aaaaaatttg atgtaacgga gagaaaatta ttaaacgtta
     2761 catgtatttt ttgatcgtat cgagaaaaat gcaaaaaata tcaaattgta atttttccgc
     2821 acatctccgc aacacacaat tttaaaaaaa tcccttatat ttcagctaac cacccgggat
     2881 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
     2941 gaggaccctt ggagggtacc ggttttccca gtcacgacgc gtggtaccga gctcagaaaa
     3001 aatgaccgct ccaaagaaga aacgcaaagt accgagctca gaaaaaatga cggcacctaa
     3061 aaagaaacgc aaagtaccgg tcgccgagaa gacagcccca aaaaagaagc gtaaagttcc
     3121 acgcgtcatg ggagtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     3181 tcagcgcgct gtctccaagg gagaggagct catcaaggag aacatgcaca ccaagctcta
     3241 catggaggga accgtcaaca accaccactt caagtgcacc cacgaagggg agggcaagcc
     3301 ctacgagggc acccagacca acaggattaa ggtggtggag ggaggccccc tgccgttcgc
     3361 attcgacatc ctggccacct gctttatgta cgggagcaag accttcatca accacaccca
     3421 aggaatccca gacttcttca agcaatcctt cgtaagttta aacatgattt tactaactaa
     3481 ctaatctgat ttaaattttc agcctgaggg cttcacatgg gagagagtca ccacatacga
     3541 agacgggggc gtgcttaccg ttacccagga caccagcctc caggacggct gcttgatcta
     3601 caacgtcaag ctcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac
     3661 actcggctgg gaggccagta ccgagaccct gtaccccgct gacggcggcc tggaaggcag
     3721 atgcgacatg gccctgaagc tcgtgggcgg gggccacctg cactgcaacc tgaagaccac
     3781 atacagatcc aagaaacccg ctaagaacct caagatgcca ggagtaagtt taaacatata
     3841 tatactaact aaccctgatt atttaaattt tcaggtctac tttgtggacc gcagactgga
     3901 aagaatcaag gaggccgaca atgagaccta cgtcgagcag cacgaggtgg ctgtggccag
     3961 atactgcgac ctccctagca aactggggca caaacttaat ggcatggacg agctgtacaa
     4021 gtaagcgccg gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg
     4081 tcatcagagt aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc
     4141 atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc
     4201 tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct
     4261 ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag
     4321 aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt
     4381 ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa
     4441 tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc
     4501 agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat
     4561 gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg
     4621 aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag
     4681 gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga
     4741 agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga
     4801 gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata
     4861 actgtttcct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     4921 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     4981 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     5041 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     5101 gcacagatgc gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc
     5161 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5221 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5281 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5341 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5401 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5461 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5521 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5581 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5641 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5701 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5761 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5821 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5881 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5941 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6001 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6061 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6121 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6181 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6241 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6301 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6421 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6481 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6541 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6601 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6661 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6721 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6781 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6841 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6901 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6961 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7021 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7081 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgatgaa gcggaagagc
     7141 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7201 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7261 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7321 tgagcggata acaatt
//
