LOCUS       sequence-441737-        8205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MAC-tagged CCDC22 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Laulumaa S, Kumpula EP, Huiskonen JT, Varjosalo M
  TITLE     Structure and interactions of the endogenous human Commander 
            complex.
  JOURNAL   Nat Struct Mol Biol. 2024 Jun;31(6):925-938. doi: 
            10.1038/s41594-024-01246-1. Epub 2024 Mar 8.
  PUBMED    38459129
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-024-01246-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2024-06"; volume: "31"; issue: "6"; pages: "925-938"
COMMENT     Sequence Label: MAC_C_CCDC22
FEATURES             Location/Qualifiers
     source          1..8205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2708..2732)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2779..2802
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2839..2862
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2893..2919
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2893..2919
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(2920..3267,3268..3270,3271..3879)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(3923..3940)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3929..4153
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4286..4305)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4437..4484
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4492..5514
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4492..5514
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5644..5765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5681..5700)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5735..5754
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5814..5830)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5814..5830)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5827..5849)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5838..5854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5862..5868,5869..5886,5887..5892))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5907..5928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6045..6062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6216..6804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6296..6315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6975..7766,7767..7835))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7598..7617
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7836..7940)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8015..8034)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag ttggcatgga ggaggcggac
      841 cgaatcctca tccattcgct gcgccaggcc ggcacggcag ttcctccaga tgtgcagacc
      901 ttgcgcgcct tcaccactga gctggttgta gaggctgtgg tccgctgcct gcgtgtgatc
      961 aaccctgcgg tgggctctgg cctcagccct ctgctgcctc ttgccatgtc tgcccggttc
     1021 cgcctggcca tgagcctggc tcaggcctgc atggacctgg gctatccctt ggagcttggc
     1081 tatcagaact tcctctaccc cagtgagcct gacctccgag acctgcttct cttcttggct
     1141 gagcgtctgc ccaccgatgc ctctgaggat gcagaccagc ctgcaggtga ctcagctatt
     1201 ctcctccggg ccattgggag ccaaattcgg gaccagctgg cactgccttg ggtcccgccc
     1261 caccttcgca ctcccaagct gcagcacctc cagggctcgg ccctccagaa gcctttccat
     1321 gccagcaggc tggtcgtgcc agaattgagt tccagaggtg agccacggga gttccaggcg
     1381 agtcccctgc tgcttccagt ccctacccag gtgcctcagc ctgttggaag ggtggcctcg
     1441 ctcctcgaac accatgccct gcagctctgc cagcagacgg gccgggaccg gccaggggat
     1501 gaggactggg tccaccggac atcccgcctc ccaccccagg aggacacacg ggctcagcgg
     1561 cagcggctgc agaagcaact gactgagcat ctgcgccaaa gctggggcct gcttggggcc
     1621 cccatacaag cccgggacct gggagaactg ctgcaggcct ggggtgctgg ggccaagact
     1681 ggtgctccta agggctcccg cttcacgcac tcagagaagt tcaccttcca tctggagccc
     1741 caggcccagg ccactcaggt gtcagatgtg ccagccacct cccggcggcc tgaacaggtc
     1801 acgtgggcag ctcaggaaca ggagctcgag tcccttcggg agcagctgga aggagtgaac
     1861 cgcagcattg aggaggttga ggccgacatg aagaccctgg gcgtcagctt tgtgcaggca
     1921 gagtctgagt gccggcacag caagctcagt acagcagagc gtgagcaggc cctgcgcctg
     1981 aagagccgcg cggtggagct gctgcccgat gggactgcca accttgccaa gctgcagctt
     2041 gtggtggaga atagtgccca gcgggtcatc cacttggcgg gtcagtggga gaagcaccgg
     2101 gtcccactcc tcgctgagta ccgccacctc cgaaagctgc aggattgcag agagctggaa
     2161 tcttctcgac ggctggcaga gatccaagaa ctgcaccaga gtgtccgggc ggctgctgaa
     2221 gaggcccgca ggaaggagga ggtctataag cagctgatgt cagagctgga gactctgccc
     2281 agagatgtgt cccggctggc ctacacccag cgcatcctgg agatcgtggg caacatccgg
     2341 aagcagaagg aagagatcac caagatcttg tctgatacga aggagcttca gaaggaaatc
     2401 aactccctat ctgggaagct ggaccggacg tttgcggtga ctgatgagct tgtgttcaag
     2461 gatgccaaga aggacgatgc tgttcggaag gcctataagt atctagctgc tctgcacgag
     2521 aactgcagcc agctcatcca gaccatcgag gacacaggca ccatcatgcg ggaggttcga
     2581 gacctcgagg agcagatcga gacagagctg ggcaagaaga ccctcagcaa cctggagaag
     2641 atccgggagg actaccgagc cctccgccag gagaacgctg gcctcctagg ccgggtccgg
     2701 gaggcctgcc caactttctt gtacaaagtg gttgatatca aggccgccga catcaccagc
     2761 ctgtacaaga aagtggggtg gagccatccc cagttcgaga agggcggagg atctggcggc
     2821 ggaagcggcg gaggaagctg gtcccaccct cagtttgaga aaggcaccga gctgggcagc
     2881 accatggcca gctaccccta cgacgtgccc gactacgcca aggacaacac cgtgcccctg
     2941 aagctgatcg ccctgctggc caacggcgag ttccactctg gcgagcagct gggcgagaca
     3001 ctgggcatga gcagagccgc catcaacaag cacatccaga ccctgcggga ctggggcgtg
     3061 gacgtgttca cagtgcccgg caagggctac agcctgcccg agcccatcca gctgctgaac
     3121 gccaagcaga tcctgggcca gctggatggc ggatctgtgg ccgtgctgcc cgtgatcgac
     3181 agcaccaacc agtatctgct cgaccggatc ggcgagctga agtccggcga cgcctgtatc
     3241 gccgagtacc agcaggccgg cagaggcggc aggggcagaa agtggttcag ccccttcggc
     3301 gccaacctgt acctgagcat gttctggaga ctggaacagg gcccagccgc cgctattggc
     3361 ctgagcctgg tcatcggcat cgtgatggcc gaggtgctgc ggaagctggg cgccgacaaa
     3421 gtgcgcgtga agtggcccaa cgacctgtat ctgcaggacc ggaagctggc cggcatcctg
     3481 gtggaactga ccggcaaaac aggcgacgcc gcccagatcg tgatcggcgc tggcatcaac
     3541 atggccatgc ggcgggtgga agagagcgtg gtcaaccagg gctggatcac cctgcaggaa
     3601 gccggcatca acctggaccg gaacaccctg gccgccatgc tgatcagaga gctgagagcc
     3661 gccctggaac tgttcgagca ggaaggcctg gccccctacc tgagcagatg ggagaagctg
     3721 gacaacttca tcaaccggcc cgtgaaactg atcatcggcg acaaagagat cttcggcatc
     3781 agccggggca tcgacaagca gggggccctg ctcctggaac aggacggcat catcaagccc
     3841 tggatgggcg gcgagatcag cctgcggagc gccgagaagt gatgactcga gtctagaggg
     3901 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3961 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4021 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4081 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4141 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4261 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4321 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4381 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tacctagaag
     4441 ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc caaaaagcct
     4501 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     4561 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     4621 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     4681 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     4741 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     4801 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     4861 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     4921 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     4981 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     5041 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     5101 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     5161 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     5221 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     5281 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     5341 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     5401 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     5461 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgta
     5521 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     5581 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     5641 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5701 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5761 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     5821 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     5881 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5941 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6001 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6061 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6121 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6181 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6241 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6301 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6361 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6421 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6481 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6541 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6601 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6661 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6721 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6781 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6841 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6901 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6961 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7021 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7081 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7141 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7201 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7261 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7321 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7381 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7441 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7501 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7561 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7621 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7681 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7741 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7801 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7861 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7921 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     7981 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     8041 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     8101 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     8161 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
