LOCUS       sequence-441868-       11507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SV2A HaloTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11507)
  TITLE     Chapman Lab SV2A plasmids
REFERENCE   2  (bases 1 to 11507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFSynW SV2A HaloTag
FEATURES             Location/Qualifiers
     source          1..11507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3059..3068
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3653..4537
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEIS"
     misc_feature    6199..6787
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6252..6272)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6670..6681)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6790..6806)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6791..6807)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7312..7492
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7524..7748
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7794..8222
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7881..7900)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8091..8112
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8231..8251)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8236..8565
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8416..8551
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8478..8497
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8613..8660
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8679..9053
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9183..9304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9220..9239)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9274..9293
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9353..9369)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9353..9369)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9366..9388)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9377..9393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9401..9407,9408..9425,9426..9431))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9446..9467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9584..9601)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9755..10343)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9835..9854)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10514..11305,11306..11374))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11137..11156
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11375..11479)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctaggc
     3061 caccatggaa gaaggctttc gagaccgagc agcgttcatc cgtggggcca aagacattgc
     3121 caaggaagtt aagaagcacg cggccaagaa ggtggtgaag ggtctcgaca gagtccagga
     3181 tgaatattcc cgaaggtcct actcccgctt tgaggaggag gaggatgatg atgacttccc
     3241 tgcccctgct gacggctatt accgcggaga aggggcccag gatgaggagg aaggtggcgc
     3301 ttccagtgat gccactgagg gccacgatga ggatgatgag atctacgagg gagaatatca
     3361 gggcatcccc cgggcagagt ctgggggcaa aggcgaacgg atggcagatg gggcacccct
     3421 ggctggagtg agagggggct taagtgatgg ggagggtccc cctgggggtc gcggggaggc
     3481 gcagcggcgt aaagatcggg aagaattggc tcagcagtat gagaccatcc tccgggagtg
     3541 cggccatggt cgcttccagt ggacactcta cttcgtgctg ggtctggcgc tgatggccga
     3601 tggtgtagag gtctttgtgg tgggctttgt gctgcccagt gctgagaaag atatggaaat
     3661 cggtactggc tttccattcg acccccatta tgtggaagtc ctgggcgagc gcatgcacta
     3721 cgtcgatgtt ggtccgcgcg atggcacccc tgtgctgttc ctgcacggta acccgacctc
     3781 ctcctacgtg tggcgcaaca tcatcccgca tgttgcaccg acccatcgct gcattgctcc
     3841 agacctgatc ggtatgggca aatccgacaa accagacctg ggttatttct tcgacgacca
     3901 cgtccgcttc atggatgcct tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat
     3961 tcacgactgg ggctccgctc tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa
     4021 aggtattgca tttatggagt tcatccgccc tatcccgacc tgggacgaat ggccagaatt
     4081 tgcccgcgag accttccagg ccttccgcac caccgacgtc ggccgcaagc tgatcatcga
     4141 tcagaacgtt tttatcgagg gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt
     4201 cgagatggac cattaccgcg agccgttcct gaatcctgtt gaccgcgagc cactgtggcg
     4261 cttcccaaac gagctgccaa tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga
     4321 atacatggac tggctgcacc agtcccctgt cccgaagctg ctgttctggg gcaccccagg
     4381 cgttctgatc ccaccggccg aagccgctcg cctggccaaa agcctgccta actgcaaggc
     4441 tgtggacatc ggcccgggtc tgaatctgct gcaagaagac aacccggacc tgatcggcag
     4501 cgagatcgcg cgctggctgt ctactctgga gatttcctgc ctgtcggact ccaacaaagg
     4561 catgctaggc ctcattgtgt acctgggcat gatggtgggg gccttcctct ggggaggcct
     4621 ggctgatcgg ctgggtcgga gacagtgtct gctcatctcg ctctcagtca acagcgtctt
     4681 cgctttcttc tcatccttcg tccagggtta tggcaccttc cttttctgcc gcctcctttc
     4741 tggggttggg attggtggtt ccatccccat tgtcttctcc tatttttcgg agtttctggc
     4801 ccaggagaaa cgtggggagc atttgagctg gctctgtatg ttctggatga ttggtggcgt
     4861 gtatgcagct gcaatggcct gggccatcat cccccactat gggtggagtt tccagatggg
     4921 ctctgcttac cagttccaca gctggagggt ctttgtcctc gtctgtgcct ttccctctgt
     4981 gtttgccatc ggggctctga ctacgcagcc ggagagtccc cgcttcttct tagagaatgg
     5041 gaagcacgat gaggcctgga tggtgctgaa gcaggttcat gacaccaaca tgcgagccaa
     5101 gggccatcct gagcgagtct tctcagtaac ccacattaaa acgattcatc aggaggatga
     5161 attgattgag atccagtcag acacaggaac ctggtaccag cgctggggag tgcgggcttt
     5221 gagcctgggg ggtcaggttt gggggaactt cctctcctgc ttcagtccag agtaccggcg
     5281 catcactctg atgatgatgg gggtatggtt caccatgtcc ttcagctact acggtttgac
     5341 tgtctggttt cccgacatga tccgccatct ccaggctgtg gactatgcag cccgaaccaa
     5401 agtgttccca ggggagcgcg tggagcacgt gacatttaac ttcacactgg agaatcagat
     5461 ccaccgaggg ggacagtact tcaatgacaa gttcatcggg ctgcgtctga agtcagtgtc
     5521 ctttgaggat tccctgtttg aggaatgtta ctttgaagat gtcacatcca gcaacacatt
     5581 cttccgcaac tgcacattca tcaacaccgt gttctacaac acggacctgt ttgagtacaa
     5641 gttcgtgaac agccgcctgg tgaacagcac attcctgcac aataaggaag gttgcccact
     5701 agatgtgaca gggacgggcg aaggtgccta catggtgtac tttgtcagct tcttggggac
     5761 actggctgtg ctccctggaa atattgtgtc tgctctgctc atggacaaga ttggcaggct
     5821 cagaatgctt gctggttcca gtgtgttgtc ctgtgtttcc tgcttcttcc tgtcttttgg
     5881 gaacagtgag tcagccatga tcgctctgct ctgccttttt gggggagtca gtattgcatc
     5941 ctggaacgcg ctggacgtgc tgactgttga actctaccct tccgacaaga ggacgacggc
     6001 cttcggcttc ctgaatgccc tgtgtaagct ggcagctgta ctgggcatca gcatcttcac
     6061 gtcctttgtg ggaatcacca aggccgctcc catcctcttc gcctcagctg cgcttgccct
     6121 tggtagctct ctggctctga agctgcctga gacccgggga caggtgctgc agtaaaattc
     6181 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6241 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6301 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6361 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6421 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6481 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6541 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6601 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6661 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6721 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6781 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
     6841 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     6901 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     6961 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7021 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     7081 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     7141 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     7201 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     7261 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     7321 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7381 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7441 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     7501 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     7561 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     7621 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     7681 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     7741 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     7801 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7861 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     7921 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     7981 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8041 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8101 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8161 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8221 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     8281 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8341 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     8401 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8461 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     8521 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     8581 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     8641 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     8701 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     8761 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     8821 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     8881 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     8941 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     9001 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     9061 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     9121 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     9181 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     9241 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9301 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     9361 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     9421 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     9481 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     9541 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9601 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9661 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9721 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9781 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9841 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9901 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9961 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10021 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10081 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10141 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    10201 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    10261 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    10321 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    10381 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    10441 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    10501 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    10561 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10621 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10681 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10741 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10801 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10861 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10921 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10981 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    11041 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    11101 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    11161 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    11221 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    11281 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    11341 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    11401 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    11461 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
