LOCUS       sequence-442068-        3963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC7A13_STOP
FEATURES             Location/Qualifiers
     source          1..3963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(271..298)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(390..476)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     525..547
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     539..556
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     540..556
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    572..671
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2091..2190)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2207..2226)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2208..2226)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2231..2247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2231..2247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2349..2368)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2360..3169
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2427..2446)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3316..3904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3805..3824
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
      241 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
      301 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
      361 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
      421 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
      481 ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg
      541 taaaacgacg gccagtctta agctcgggcc ccaaataatg attttatttt gactgatagt
      601 gacctgttcg ttgcaacaca ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      661 aaaagcaggc tccaccatgg accgcggcga gaagatccag ctgaagcggg tgtttggcta
      721 ctggtggggc accagcttcc tgctgatcaa catcatcgga gcaggaatct tcgtgagccc
      781 aaagggcgtg ctggcctatt cctgcatgaa tgtgggcgtg tctctgtgcg tgtgggcagg
      841 atgtgcaatc ctggccatga cctccacact gtgctctgcc gagatctcta tcagcttccc
      901 ttgttccggc gcccagtact atttcctgaa gagatacttt ggctctaccg tggcctttct
      961 gaacctgtgg acaagcctgt tcctgggatc cggagtggtg gcaggacagg ccctgctgct
     1021 ggccgagtat tccatccagc ccttctttcc ttcctgctct gtgccaaagc tgcccaagaa
     1081 gtgtctggcc ctggccatgc tgtggatcgt gggcatcctg accagcaggg gcgtgaagga
     1141 ggtgacatgg ctgcagatcg ccagctccgt gctgaaggtg tctatcctga gctttatctc
     1201 cctgaccggc gtggtgttcc tgatcagagg caagaaggag aacgtggaga ggttccagaa
     1261 tgcctttgac gccgagctgc ctgatatctc tcacctgatc caggccatct tccagggcta
     1321 ctttgcctat agcggcggcg cctgctttac actgatcgcc ggcgagctga agaagcctcg
     1381 caccacaatc ccaaagtgta tcttcaccgc cctgccactg gtgacagtgg tgtacctgct
     1441 ggtgaacatc tcttatctga ccgtgctgac accccgcgag atcctgtcta gcgacgcagt
     1501 ggcaatcacc tgggcagata gggcctttcc tagcctggcc tggatcatgc cattcgccat
     1561 cagcacatcc ctgttttcta atctgctgat cagcatcttc aagtcctcta ggccaatcta
     1621 cctggcatcc caggagggac agctgcctct gctgtttaac accctgaatt ctcacagctc
     1681 cccattcacc gccgtgctgc tgctggtgac actgggcagc ctggccatca tcctgacctc
     1741 cctgatcgac ctgatcaact acatcttctt tacaggctct ctgtggagca tcctgctgat
     1801 gatcggcatc ctgcggagaa ggtaccagga gcccaatctg tctatccctt ataaggtgtt
     1861 cctgagcttt cccctggcca ccatcgtgat cgatgtgggc ctggtggtca tcccactggt
     1921 gaagagcccc aacgtgcact acgtgtatgt gctgctgctg gtgctgtccg gcctgctgtt
     1981 ctacatccct ctgatccact ttaagatcag actggcctgg ttcgagaaga tgacatgcta
     2041 tctgcagctg ctgttcaata tctgtctgcc agacgtgagc gaggagtaag acccagcttt
     2101 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2161 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2221 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2281 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     2341 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     2401 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     2461 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     2521 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     2581 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     2641 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     2701 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     2761 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     2821 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     2881 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     2941 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3001 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3061 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3121 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     3181 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     3241 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     3301 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3361 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3421 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3481 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3541 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3601 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3661 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3721 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3781 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3841 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3901 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3961 cat
//
