LOCUS       sequence-442124-        3636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The backbone vector for rice genome editing is based on PrCas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Liu S, He Y, Fan T, Zhu M, Qi C, Ma Y, Yang M, Yang L, Tang X, Zhou 
            J, Zhong Z, An X, Qi Y, Zhang Y
  TITLE     PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single 
            transcript unit systems for efficient singular and multiplexed 
            genome editing in rice, maize, and tomato.
  JOURNAL   Plant Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486.
  PUBMED    39387219
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486."
COMMENT     Sequence Label: pGEL1023
FEATURES             Location/Qualifiers
     source          1..3636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atcattggcc gggatttcaa tatgtactac cagaacctca ccaagatgta cccaatctcc
       61 aagacactca ggaacgagct gattcctgtg ggcaagaccc tggagaatat ccgcaagaac
      121 ggcattctgg aggccgacat ccagcggaag gcggattacg agcacgtgaa gaagctcatg
      181 gacaactacc ataagcagct catcaacgag gccctccagg gcgtccacct ctcagacctg
      241 tctgatgcgt acgacctcta cttcaacctg tccaaggaga agaatagcgt ggatgccttc
      301 tcaaagtgcc aggacaagct ccgcaaggag atcgtcagcc tcctgaagaa ccatgagaat
      361 ttcccaaaga ttggcaacaa ggagatcatt aagctcctgc agtcactcta cgacaatgat
      421 accgactaca aggcgctgga ctccttcagc aacttctaca catacttctc cagctacaat
      481 gaggtgagga agaacctcta ctccgacgag gagaagtcat ctacagtcgc ctaccgcctg
      541 atcaacgaga atctccctaa gttcctggac aacattaagg cctacgcgat cgccaagaag
      601 gccggcgtgc gcgccgaggg cctctcagag gaggatcagg actgcctgtt catcattgag
      661 acattcgagc ggaccctcac acaggatggc attgacaact acaatgccgc gatcggcaag
      721 ctcaacacag cgattaacct gttcaatcag cagaacaaga agcaggaggg cttccggaag
      781 gtccctcaga tgaagtgcct ctacaagcag atcctgtctg atagggagga ggccttcatt
      841 gacgagttct ccgacgatga ggatctcatc accaatattg agagcttcgc ggagaacatg
      901 aatgtgttcc tgaactcaga gatcattaca gacttcaaga tcgccctcgt cgagtccgat
      961 ggcagcctgg tgtacattaa gaatgacgtg tccaagacct ccttctccaa catcgtgttc
     1021 ggctcctgga atgccattga tgagaagctc tccgacgagt acgacctggc gaactccaag
     1081 aagaagaagg acgagaagta ctacgagaag cgccagaagg agctgaagaa gaacaagtcc
     1141 tacgacctgg agacaatcat cggcctgttc gacgataact ctgatgtgat cggcaagtac
     1201 attgagaagc tggagtccga catcacagcg attgccgagg cgaagaatga tttcgacgag
     1261 atcgtcctca ggaagcacga caagaacaag agcctgcgca agaacaccaa tgccgtggag
     1321 gcgattaagt catacctcga tacagtcaag gacttcgaga gggatatcaa gctgattaat
     1381 ggctctggcc aggaggtgga gaagaacctc gtggtctacg ccgagcagga gaacatcctg
     1441 gcggagatta agaatgtcga ttctctctac aatatgtcca ggaactacct gacccagaag
     1501 cctttctcca cagagaagtt caagctcaac ttcaatcgcg cgaccctcct gaatggctgg
     1561 gacaagaaca aggagacaga taatctcggc atcctgttcg agaaggatgg catgtactac
     1621 ctcggcatta tgaataccaa ggccaacaag atcttcgtga acattccgaa ggcgacatcc
     1681 aatgacgtgt accacaaggt caactacaag ctcctgccgg gcccaaataa gatgctcccg
     1741 aaggtgttct tcgcccagtc taacctggac tactacaagc catccgagga gctgctggcc
     1801 aagtacaagg cgggcaccca caagaagggc gacaacttct ccctggagga ctgccatgcc
     1861 ctgatcgact tcttcaaggc gtccattgag aagcatccag attggtccag cttcggcttc
     1921 gagttcagcg agacatgcac atacgaggac ctctcaggct tctacaggga ggtggagaag
     1981 cagggctaca agatcaccta cacagatgtg gacgccgatt acattaccag cctggtcgag
     2041 agggacgagc tgtacctgtt ccagatctac aataaggact tctcaccgta ctctaagggc
     2101 aacctcaatc tgcacacaat ctacctccag atgctgttcg atcagcgcaa tctcaacaat
     2161 gtggtctaca agctgaacgg cgaggcggag gtgttctaca ggccagcgag catcaacgat
     2221 gaggaggtca tcattcacaa ggccggcgag gagattaaga acaagaattc taagagggcg
     2281 gtggacaagc caacctccaa gttcggctac gacatcatta aggataggcg ctactcaaag
     2341 gataagttca tgctccatat ccctgtgacc atgaacttcg gcgtcgacga gacacggagg
     2401 ttcaatgacg tggtcaacga tgcgctgcgg aatgatgaga aggtgagggt catcggcatt
     2461 gacaggggcg agcgcaacct cctctacgtg gtcgtggtcg acaccgatgg cacaatcctg
     2521 gagcagattt ccctgaatag catcattaac aatgagtact ctatcgagac agattaccat
     2581 aagctcctgg acgagaagga gggcgaccgc gatcgggcca ggaagaactg gaccacaatc
     2641 gagaatatta aggagctgaa ggagggctac ctgtcccagg tggtcaacgt gatcgccaag
     2701 ctcgtcctga agtacaatgc gatcatttgc ctggaggacc tcaacttcgg cttcaagcgc
     2761 ggccggcaga aggtggagaa gcaggtctac cagaagttcg agaagatgct catcgacaag
     2821 ctcaattacc tcgtgatcga taagtcccgc aagcaggaca agccagagga gttcggcggc
     2881 gccctgaacg cgctccagct gacctcaaag ttcacatctt tcaaggatat gggcaagcag
     2941 accggcatca tctactacgt ccctgcctac ctcacatcca agatcgaccc gaccacaggc
     3001 ttcgcgaatc tgttctacgt gaagtacgag aacgtcgaga aggccaagga gttcttcagc
     3061 aggttcgatt ccatcagcta caacaatgag tcaggctact tcgagttcgc cttcgattac
     3121 aagaagttca ccgacagggc atgcggcgcg cggagccagt ggaccgtgtg cacatacggc
     3181 gagaggatca ttaagttccg caataccgag aagaacaata gcttcgacga taagacaatc
     3241 gtcctctcag aggagttcaa ggagctgttc agcatctacg gcatttcata cgaggacggc
     3301 gccgagctga agaacaagat catgtctgtg gatgaggcgg acttcttccg gtccctcacc
     3361 aggctgttcc agcagacaat gcagatgcgc aattcatcta acgacgtcac ccgggattac
     3421 atcatttccc ctatcatgaa tgatcggggc gagttcttca actctgaggc ctgcgacgcg
     3481 tccaagccaa aggacgccga tgcgaatggc gccttcaata tcgccaggaa gggcctctgg
     3541 gtgctggagc agattaggaa tacaccaagc ggcgacaagc tcaacctggc catgtcaaat
     3601 gcggagtggc tggagtacgc ccagaggaac cagatc
//
