LOCUS       sequence-44213-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB8k-GFP
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      44..115
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      131..158
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(225..887)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVLAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 actctcaata aggatccaaa ctcgagtaag gatctccagg catcaaataa aacgaaaggc
       61 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      121 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatacctagg ctacagccga
      181 tagtctggaa cagcgcactt acgggttgct gcgcaaccca agtgctaccg gcgcggcagc
      241 gtgacccgtg tcggcggctc caacggctcg ccatcgtcca gaaaacacgg ctcatcgggc
      301 atcggcaggc gctgctgccc gcgccgttcc cattcctccg tttcggtcaa ggctggcagg
      361 tctggttcca tgcccggaat gccgggctgg ctgggcggct cctcgccggg gccggtcggt
      421 agttgctgct cgcccggata cagggtcggg atgcggcgca ggtcgccatg ccccaacagc
      481 gattcgtcct ggtcgtcgtg atcaaccacc acggcggcac tgaacaccga caggcgcaac
      541 tggtcgcggg gctggcccca cgccacgcgg tcattgacca cgtaggccaa cacggtgccg
      601 gggccgttga gcttcacgac ggagatccag cgctcggcca ccaagtcctt gactgcgtat
      661 tggaccgtcc gcaaagaacg tccgatgagc ttggaaagtg tcttctggct gaccaccacg
      721 gcgttctggt ggcccatctg cgccacgagg tgatgcagca gcattgccgc cgtgggtttc
      781 ctcgcaataa gcccggccca cgcctcatgc gctttgcgtt ccgtttgcac ccagtgaccg
      841 ggcttgttct tggcttgaat gccgatttct ctggactgcg tggccatgc
//
