LOCUS       sequence-442130-        3783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The STU backbone vector for tomato genome editing is based on 
            Mb3Cas12a-RRR (D172R/N573R/K579R).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Liu S, He Y, Fan T, Zhu M, Qi C, Ma Y, Yang M, Yang L, Tang X, Zhou 
            J, Zhong Z, An X, Qi Y, Zhang Y
  TITLE     PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single 
            transcript unit systems for efficient singular and multiplexed 
            genome editing in rice, maize, and tomato.
  JOURNAL   Plant Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486.
  PUBMED    39387219
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486."
COMMENT     Sequence Label: pGEL1029
FEATURES             Location/Qualifiers
     source          1..3783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctcttcc aggacttcac ccacctctac ccactgtcca agacagtgcg gttcgagctg
       61 aagcctatcg gcaagaccct cgagcacatt catgccaaga acttcctcaa tcaggacgag
      121 acaatggccg atatgtacca gaaggtcaag gcgatcctgg acgattacca tagggacttc
      181 attgccgata tgatgggcga ggtgaagctc accaagctgg cggagttcta cgatgtctac
      241 ctgaagttcc gcaagaaccc aaaggacgat ggcctccaga agcagctcaa ggacctccag
      301 gccgtgctgc ggaaggagat cgtcaagcct attggcaatg gcggcaagta caaggccggc
      361 tacgataggc tgttcggcgc gaagctcttc aaggacggca aggagctcgg cgatctggcc
      421 aagttcgtga ttgcccagga gggagagtcc agccctaagc tcgcccacct ggcgcatttc
      481 gagaagttct caacctactt cacaggcttc cacaggaaca ggaagaatat gtactctgac
      541 gaggataagc acaccgccat cgcgtaccgg ctgattcatg agaacctccc gaggttcatt
      601 gataatctcc agatcctggc caccattaag cagaagcatt ccgcgctgta cgaccagatc
      661 attaacgagc tcacagccag cggcctcgat gtctcactgg cgtctcacct cgacggctac
      721 cataagctcc tgacacagga gggcatcacc gcctacaata cactcctggg cggcatttca
      781 ggagaggccg gcagccgcaa gatccagggc attaacgagc tcatcaattc acaccataac
      841 cagcactgcc ataagtctga gaggatcgcc aagctccgcc cgctgcacaa gcagattctg
      901 tccgatggca tgggcgtgtc cttcctccca agcaagttcg ccgacgatag cgaggtgtgc
      961 caggcggtca acgagttcta ccggcattac gccgacgtgt tcgcgaaggt ccagtccctc
     1021 ttcgatggct tcgacgatta ccagaaggac ggcatctacg tcgagtacaa gaacctcaat
     1081 gagctgagca agcaggcctt cggcgatttc gccctcctgg gcagggtgct ggatggatac
     1141 tacgtcgatg tggtcaatcc ggagttcaac gagcgcttcg ccaaggcgaa gaccgataac
     1201 gccaaggcga agctcacaaa ggagaaggac aagttcatta agggagtgca ctcactggcc
     1261 agcctggagc aggccattga gcactacacc gccaggcatg acgatgagtc agtgcaggcc
     1321 ggcaagctgg gacagtactt caagcatggc ctcgccggcg tggacaaccc aatccagaag
     1381 attcacaaca atcatagcac aatcaagggc ttcctcgagc gggagcgccc ggccggcgag
     1441 agggccctgc caaagatcaa gtccgataag tctccagaga ttcggcagct gaaggaactc
     1501 ctggacaacg ccctcaatgt cgcccacttc gcgaagctcc tgaccacaaa gaccacactc
     1561 cataatcagg atggcaactt ctacggcgag ttcggcgcgc tgtacgacga gctcgccaag
     1621 atcgcgaccc tgtacaacaa ggtgcgcgat tacctctcac agaagccttt ctctaccgag
     1681 aagtacaagc tcaacttcgg caatccgaca ctcctgcggg gctgggacct gaacagggag
     1741 aaggataatt tcggcgtcat cctgcagaag gacggctgct actacctcgc cctcctggat
     1801 aaggcgcaca agaaggtgtt cgacaacgcc ccgaatacag gcaagagcgt ctaccagaag
     1861 atgatctaca agctcctgcc gggcccaaac aagatgctgc caaaggtgtt cttcgcgaag
     1921 tccaacctcg attactacaa tcctagcgcc gagctcctgg acaagtacgc gcagggcacc
     1981 cacaagaagg gcgacaactt caatctgaag gattgccatg ccctcatcga cttcttcaag
     2041 gcgggcatta ataagcaccc ggagtggcag catttcggct tcaagttctc cccaacatca
     2101 tcttaccagg acctcagcga tttctaccgc gaggtggagc cacagggcta ccaggtgaag
     2161 ttcgtggaca tcaacgccga ttacattaat gagctcgtcg agcagggcca gctctacctg
     2221 ttccagatct acaacaagga tttctcccca aaggcccacg gcaagcctaa tctccatacc
     2281 ctgtacttca aggcgctgtt cagcgaggac aacctcgtga atcctatcta caagctcaac
     2341 ggcgaggccg agattttcta ccggaaggcg tccctggata tgaacgagac aacaattcac
     2401 agggccggcg aggtgctcga gaacaagaat cctgacaacc cgaagaagcg ccagttcgtc
     2461 tacgacatca ttaaggataa gcggtacaca caggataagt tcatgctcca tgtgccgatc
     2521 accatgaatt tcggcgtcca gggcatgaca attaaggagt tcaataagaa ggtgaaccag
     2581 tcaatccagc agtacgatga ggtgaacgtc attggaattg atcgcggcga gaggcacctc
     2641 ctgtacctca ccgtcatcaa ttctaagggc gagattctgg agcagaggtc actcaacgat
     2701 atcaccacag cctctgcgaa tggcacacag atgaccacac cataccataa gatcctggac
     2761 aagagggaga ttgagaggct caatgcccgg gtgggatggg gcgagatcga gacaatcaag
     2821 gagctgaagt caggctacct ctctcacgtg gtccatcaga tcagccagct catgctgaag
     2881 tacaacgcca ttgtggtcct cgaggacctg aatttcggct tcaagcgggg caggttcaag
     2941 gtggagaagc agatctacca gaacttcgag aatgcgctga ttaagaagct caaccacctc
     3001 gtcctgaagg ataaggccga cgatgagatc ggctcataca agaatgcgct ccagctgacc
     3061 aacaatttca cagacctgaa gtctatcggc aagcagaccg gcttcctctt ctacgtgcct
     3121 gcctggaaca cctcaaagat tgatccggag acaggcttcg tggacctcct gaagcctcgc
     3181 tacgagaaca ttgcccagtc tcaggcgttc ttcggcaagt tcgataagat ctgctacaat
     3241 gcggaccggg gctacttcga gttccacatt gattacgcca agttcaacga caaggcgaag
     3301 aattccaggc agatctggaa gatttgcagc catggcgata agcgctacgt gtacgacaag
     3361 accgccaacc agaataaggg cgcgacaatc ggcgtgaacg tcaatgatga gctcaagtca
     3421 ctgttcaccc ggtaccacat taacgacaag cagccaaatc tcgtcatgga tatctgccag
     3481 aacaatgaca aggagttcca taagtctctg atgtacctcc tgaagacact cctggccctc
     3541 aggtactcaa acgcctccag cgacgaggat ttcatcctct ctcctgtggc caacgatgag
     3601 ggcgtcttct tcaattcagc actggccgat gatacacagc cacagaatgc ggatgccaac
     3661 ggcgcatacc acattgccct caagggcctg tggctcctga atgagctcaa gaactcagac
     3721 gatctgaaca aggtgaagct cgccattgac aatcagacat ggctgaattt cgcgcagaac
     3781 cgc
//
