LOCUS       sequence-442457-       11340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the introduction of Mariner transposon that inserts 
            deoxyviolacein cassette behind indigenous promoters in target 
            bacterial strain. Plasmid has tetracycline marker for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11340)
  TITLE     A Deoxyviolacein-Based Transposon Insertion Vector for Pigmented 
            Tracer Studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBB328
FEATURES             Location/Qualifiers
     source          1..11340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            92..200
                     /note="incP origin of transfer"
     primer_bind     complement(288..305)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(288..304)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(297..319)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     RBS             724..732
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             1998..2006
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             5018..5026
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             6331..6339
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(6955..8154)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             8260..8910
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      9073..9461
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     primer_bind     complement(11184..11206)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11195..11211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11219..11225,11226..11243,11244..11249))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11264..11285
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag
       61 actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg
      121 cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc
      181 ctgctctgcg aggctggccg ataagctcta agaaaccatt attatcatga cattaaccta
      241 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttaattcact ggccgtcgtt
      301 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct cgatcgttgt
      361 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
      421 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
      481 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
      541 cgcgccacat aacaggttgg atgataagtc cccggtctaa caaagaaaaa cacatttttt
      601 tgtgaaaatt cgtttttatt attcaacata gttcccttca agagcgatac ccctcgaatt
      661 gacgcgtcga attaattccg cgaaccccag gatccaggtc gactctagat atccatggat
      721 ctttaaggag gtaaaaaaaa tgaaacattc ttccgatatc tgcattgttg gtgctggtat
      781 ttctggtttg acgtgcgcaa gccatctgct ggacagcccg gcatgccgtg gtctgagcct
      841 gcgtatcttt gacatgcagc aagaagccgg tggccgtatc cgcagcaaaa tgctggatgg
      901 taaggcaagc attgaactgg gcgcaggtcg ctactcccct cagttgcacc cgcatttcca
      961 aagcgcaatg cagcactata gccaaaagag cgaagtctat ccgttcaccc agttgaagtt
     1021 caaatctcac gtgcagcaaa agctgaagcg cgccatgaat gaactgtccc cgcgtctgaa
     1081 agagcatggt aaagagagct ttttgcagtt tgtcagccgt tatcaaggtc acgatagcgc
     1141 ggttggtatg atccgctcta tgggttacga cgcactgttc ctgccggata tcagcgcaga
     1201 aatggcctac gacattgtgg gtaagcaccc ggagatccag agcgtgacgg acaacgacgc
     1261 gaaccaatgg tttgcagcgg aaacgggctt tgctggtctg attcagggca tcaaggctaa
     1321 ggttaaggcg gcaggtgcgc gttttagcct gggttatcgt ctgctgagcg tccgtaccga
     1381 cggtgacggc tacctgctgc aactggcagg tgacgacggc tggaaactgg agcaccgtac
     1441 ccgccatctg attctggcga ttccgccgag cgcgatggcg ggtttgaatg ttgattttcc
     1501 agaagcctgg tccggtgcgc gctatggcag cctgccgctg tttaagggct ttctgacgta
     1561 cggtgagccg tggtggttgg actacaaact ggacgatcag gtgctgattg ttgacaaccc
     1621 gctgcgcaaa atctatttca aaggcgataa gtacctgttc ttctataccg atagcgagat
     1681 ggcgaattac tggcgcggtt gtgtcgcgga gggcgaggac ggttacctgg agcaaattcg
     1741 cacccatttg gctagcgcac tgggtatcgt ccgtgaacgt atcccgcaac cgctggcaca
     1801 cgttcacaag tattgggcgc acggcgttga gttttgccgt gattctgata ttgaccaccc
     1861 gagcgcactg tctcatcgcg acagcggtat catcgcgtgc tccgatgcgt acacggagca
     1921 ttgtggttgg atggagggcg gtctgctgag cgcccgtgag gcaagccgtc tgctgttgca
     1981 gcgtatcgcc gcgtgattaa ggaggtaaaa aaaatgagca ttctggattt cccgcgtatc
     2041 cacttccgtg gctgggcccg tgtcaatgcg ccgaccgcga accgcgatcc gcacggccac
     2101 atcgatatgg ccagcaatac cgtggcgatg gcgggtgagc cgttcgacct ggcacgccat
     2161 cctacggagt tccaccgtca cctgcgctcc ctgggtccgc gcttcggctt ggatggtcgt
     2221 gctgacccgg aaggcccgtt cagcctggcc gagggctaca acgctgccgg taacaaccac
     2281 ttttcgtggg agagcgcaac cgttagccac gtgcaatggg atggcggtga ggcggatcgt
     2341 ggtgacggtc tggtcggtgc tcgtttggca ctgtggggtc actacaatga ttatctgcgt
     2401 accaccttca atcgtgctcg ttgggtcgac agcgacccga cgcgccgtga cgctgcacaa
     2461 atctatgcgg gccaattcac cattagcccg gctggtgccg gtccgggtac gccgtggctg
     2521 tttacggcag acattgatga tagccatggt gcacgttgga cgcgtggcgg ccacattgca
     2581 gagcgtggcg gccacttctt ggatgaagag tttggtctgg cacgcctgtt tcagttctct
     2641 gtgccgaaag atcacccaca ttttctgttt cacccgggtc cgtttgattc cgaggcctgg
     2701 cgtcgtctgc aattggctct ggaggatgac gacgttctgg gtctgaccgt gcaatatgcg
     2761 ttgttcaata tgagcacccc gcctcagccg aacagcccgg tttttcacga tatggtcggt
     2821 gttgtcggtc tgtggcgtcg tggtgaactg gcgagctacc cggctggtcg tctgctgcgt
     2881 ccgcgtcaac cgggtctggg tgacctgacc ctgcgcgtca acggtggtcg cgttgcgctg
     2941 aatttggcgt gtgccattcc gttcagcact cgtgccgcgc agccaagcgc accggaccgc
     3001 ctgaccccgg acctgggtgc caaactgccg ctgggcgatc tgctgctgcg tgatgaggac
     3061 ggcgcactgt tggcacgtgt gccgcaggct ctgtaccaag actattggac gaatcacggt
     3121 attgtggacc tgccgctgct gcgcgaaccg cgtggtagct tgaccctgag cagcgaactg
     3181 gcggagtggc gtgagcaaga ctgggtcacc caaagcgacg cgtctaacct gtacctggag
     3241 gcaccggatc gccgtcacgg tcgctttttc cctgagagca tcgcgctgcg cagctacttt
     3301 cgcggtgaag cgcgtgcgcg tccggatatc ccgcatcgta tcgagggcat gggcctggtc
     3361 ggcgtcgaat ctcgtcagga tggcgacgct gcggaatggc gtctgacggg tctgcgtccg
     3421 ggtccggcac gcattgttct ggacgatggt gccgaggcga tccctctgcg tgttctgcct
     3481 gacgattggg cgctggatga cgcgaccgtc gaagaagtgg attacgcctt tttgtaccgc
     3541 cacgttatgg cgtattacga gctggtgtat ccattcatga gcgacaaggt gttttccctg
     3601 gctgatcgtt gcaaatgtga aacgtacgca cgtctgatgt ggcagatgtg tgatccgcag
     3661 aaccgcaaca agtcctatta catgccgagc acccgcgaac tgtcggcacc gaaagctcgt
     3721 ttgttcttga agtatctggc ccacgtggaa ggccaggcac gcctgcaagc acctccgcca
     3781 gcgggtccgg cacgcattga atctaaagcc cagttggcgg cagagctgcg taaagccgtc
     3841 gacctggagc tgtctgtgat gctgcaatac ctgtacgcgg cgtatagcat tccgaactat
     3901 gcacagggcc aacaacgtgt tcgtgacggt gcgtggaccg ccgagcagct gcaactggcg
     3961 tgcggtagcg gtgaccgtcg ccgtgatggc ggtattcgtg cagcactgct ggaaattgct
     4021 catgaagaaa tgattcatta cctggtcgtt aacaacctgc tgatggccct gggcgagccg
     4081 ttctacgcgg gtgtcccgct gatgggcgaa gcggcacgtc aggcgtttgg cctggacacc
     4141 gagttcgctc tggaaccgtt tagcgaaagc acgctggcac gttttgttcg tctggaatgg
     4201 ccgcacttta tcccagcacc gggcaaatcc atcgcggact gctatgccgc cattcgtcag
     4261 gcgtttttgg atctgccgga cttgtttggt ggcgaggcag gtaagcgtgg cggtgaacac
     4321 cacctgttcc tgaatgagct gaccaaccgt gcgcatccgg gttatcaact ggaagttttc
     4381 gatcgcgact cggcgctgtt tggtattgca tttgtgaccg atcagggcga aggtggcgct
     4441 ctggacagcc cgcactacga acatagccat tttcaacgtc tgcgtgaaat gagcgcgcgt
     4501 atcatggctc aaagcgcacc gttcgaaccg gcgctgccgg cgttgcgtaa tccggttctg
     4561 gatgagagcc cgggttgcca acgtgtcgca gacggtcgtg cgcgtgcgct gatggcattg
     4621 taccaaggcg tttatgagct gatgtttgcg atgatggcgc agcacttcgc cgtgaaaccg
     4681 ctgggtagct tgcgtcgcag ccgcctgatg aacgcagcaa tcgatctgat gaccggtctg
     4741 ttgcgtccgc tgagctgcgc gctgatgaac ctgccaagcg gcatcgccgg tcgcacggcc
     4801 ggtccgccgc tgccgggtcc ggttgacacc cgtagctatg acgactacgc gctgggctgt
     4861 cgcatgctgg cacgccgttg cgagcgtctg ctggagcagg cgagcatgct ggaaccgggt
     4921 tggctgccgg atgcgcagat ggagctgctg gatttctatc gtcgccaaat gctggacttg
     4981 gcgtgcggca aactgagccg cgaggcctaa tgatccttaa ggaggtaaaa aaaatgaaac
     5041 gtgcgattat cgttggtggc ggcctggcgg gtggcctgac cgcgatctac ctggcgaagc
     5101 gtggctacga agtgcacgtc gtggagaagc gtggtgatcc tctgcgcgat ctgagctctt
     5161 acgtggacgt tgttagcagc cgtgcgatcg gcgtgagcat gaccgttcgt ggtatcaaga
     5221 gcgttttggc tgcgggcatt ccgcgtgcag agctggatgc gtgtggcgaa ccgatcgtgg
     5281 caatggcttt ctccgtgggt ggtcagtatc gcatgcgcga actgaagccg ttggaggatt
     5341 tccgtccgct gagcttgaac cgtgcggcgt ttcaaaagct gctgaacaaa tacgcgaacc
     5401 tggcaggcgt tcgttactac tttgagcata agtgcctgga tgttgacctg gatggtaaga
     5461 gcgtgttgat tcagggcaaa gatggtcagc cgcagcgtct gcaaggtgac atgattatcg
     5521 gtgcggatgg cgcccacagc gccgtccgtc aggcgatgca gagcggcctg cgtcgtttcg
     5581 agttccagca aacgttcttc cgccatggct acaaaaccct ggttttgccg gacgcgcaag
     5641 cactgggtta ccgtaaagac acgctgtact ttttcggcat ggattccggt ggcctgttcg
     5701 cgggtcgtgc ggctacgatc ccagatggta gcgtcagcat cgccgtttgc ctgccgtact
     5761 cgggtagccc ttccctgacg accaccgacg aaccgacgat gcgtgcgttc ttcgatcgtt
     5821 acttcggtgg cctgccgcgt gacgcgcgtg acgaaatgct gcgtcagttt ctggcgaagc
     5881 cgagcaacga cctgattaac gtgcgctcta gcacctttca ctataagggt aatgtgctgt
     5941 tgctgggtga tgctgcgcat gcgactgcgc cgttcctggg tcagggtatg aacatggcgc
     6001 tggaggacgc ccgcacgttt gtcgagctgc tggaccgcca ccagggcgac caagacaaag
     6061 cctttccgga gttcacggag ctgcgcaaag tccaggcaga cgcaatgcaa gacatggctc
     6121 gcgccaacta tgacgttttg agctgctcga acccgatctt tttcatgcgt gcgcgttaca
     6181 cgcgttacat gcattccaag tttccgggcc tgtatccgcc ggatatggcc gagaaactgt
     6241 actttacgag cgagccgtac gatcgtctgc aacaaatcca gcgtaaacag aatgtttggt
     6301 acaagattgg tcgcgtgaat tgatgatctt taaggaggta aaaaaaatgg agaaccgtga
     6361 gccaccactg ttgccagccc gttggagcag cgcctatgtc tcttattgga gcccgatgct
     6421 gccggatgac cagctgacca gcggctattg ctggttcgac tatgaacgtg acatctgtcg
     6481 tattgacggc ctgttcaatc cgtggagcga gcgtgatact ggttatcgcc tgtggatgtc
     6541 ggaggttggt aatgcggcca gcggccgtac ctggaaacaa aaagtcgcct atggtcgtga
     6601 gcgtaccgcc ctgggtgaac agctgtgtga gcgtccgctg gatgatgaga ctggcccttt
     6661 tgccgaattg ttcctgccac gcgatgtcct gcgccgtctg ggtgcccgtc acattggccg
     6721 tcgcgtggtt ctgggtcgcg aagcggacgg ttggcgttac cagcgcccag gtaaaggtcc
     6781 gagcaccctg tacctggatg cggcgagcgg cactccactg cgcatggtca ccggcgatga
     6841 agcgtcgcgt gcaagcctgc gtgattttcc gaatgtgagc gaggcggaga tcccggacgc
     6901 ggttttcgcg gccaagcgct aaggatccat ggcataggcc tatcgtttcc acgatcagcg
     6961 atcggctcgt tgccctgcgc cgctccaaag cccgcgacgc agcgccggca ggcagagcaa
     7021 gtagagggca gcgcctgcaa tccatgccca cccgttccac gttgttatag aagccgcata
     7081 gatcgccgtg aagaggaggg gtccgacgat cgaggtcagg ctggtgagcg ccgccagtga
     7141 gccttgcagc tgcccctgac gttcctcatc cacctgcctg gacaacattg cttgcagcgc
     7201 cggcattccg atgccacccg aagcaagcag gaccatgatc gggaacgcca tccatccccg
     7261 tgtcgcgaag gcaagcagga tgtagcctgt gccgtcggca atcattccga gcatgagtgc
     7321 ccgcctttcg ccgagccggg cggctacagg gccggtgatc attgcctggg cgagtgaatg
     7381 cagaatgcca aatgcggcaa gcgaaatgcc gatcgtggtc gcgtcccagt gaaagcgatc
     7441 ctcgccgaaa atgacccaaa gcgcggccgg cacctgtccg acaagttgca tgatgaagaa
     7501 gaccgccatc agggcggcga cgacggtcat gccccgggcc caccggaacg aagcgagcgg
     7561 gttgagagcc tcccggcgta acggccggcg ttcgcctttg tgcgactccg gcaaaaggaa
     7621 acagcccgtc aggaaattga ggccgttcaa ggctgccgcg gcgaagaacg gagcgtgggg
     7681 ggagaaacca cccatcagcc caccgagcac aggtcccgcg accatcccga acccgaaaca
     7741 ggcgctcatg aagccgaagt gccgcgcgcg ctcatcgcca tcagtgatgt cggcaatata
     7801 agcgccggct accgccccag tcgccccggt gatgccggcc acgatccgcc cgatatagag
     7861 aacccaaagg aaaggcgctg tcgccatgat ggcgtagtcg acagtggcgc cggccagcga
     7921 gacgagcaag attggccgcc gcccgaaacg atccgacagc gcgcccagca caggtgcgca
     7981 ggcaaattgc accaacgcat acagcgccag cagaatgcca tagtgggcgg tgacgtcgtt
     8041 cgagtgaacc agatcgcgca ggaggcccgg caacaccggc ataatcaggc cgatgccgac
     8101 agcgtcgagc gcgacagtgc tcagaattac gatcaggggt atgttgggtt tcacgtctgg
     8161 cctccggacc agcctccgct ggtccgattg aacgcgcgga ttctttatca ctgataagtt
     8221 ggtggacata ttatgtttat cagtgataaa gtgtcaagca tgacaaagtt gcagccgaat
     8281 acagtgatcc gtgccgccct ggacctgttg aacgaggtcg gcgtagacgg tctgacgaca
     8341 cgcaaactgg cggaacggtt gggggttcag cagccggcgc tttactggca cttcaggaac
     8401 aagcgggcgc tgctcgacgc actggccgaa gccatgctgg cggagaatca tacgcattcg
     8461 gtgccgagag ccgacgacga ctggcgctca tttctgatcg ggaatgcccg cagcttcagg
     8521 caggcgctgc tcgcctaccg cgatggcgcg cgcatccatg ccggcacgcg accgggcgca
     8581 ccgcagatgg aaacggccga cgcgcagctt cgcttcctct gcgaggcggg tttttcggcc
     8641 ggggacgccg tcaatgcgct gatgacaatc agctacttca ctgttggggc cgtgcttgag
     8701 gagcaggccg gcgacagcga tgccggcgag cgcggcggca ccgttgaaca ggctccgctc
     8761 tcgccgctgt tgcgggccgc gatagacgcc ttcgacgaag ccggtccgga cgcagcgttc
     8821 gagcagggac tcgcggtgat tgtcgatgga ttggcgaaaa ggaggctcgt tgtcaggaac
     8881 gttgaaggac cgagaaaggg tgacgattga tcaggaccgc tgccggagcg caacccagat
     8941 atctagagga tccctgcagc ccttaattaa ccccgaaaag tgccacctga cgtctaagaa
     9001 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     9061 caagaattcc catgtcagcc gttaagtgtt cctgtgtcac tcaaaattgc tttgagaggc
     9121 tctaagggct tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt taaaccttaa
     9181 aagctttaaa agccttatat attctttttt ttcttataaa acttaaaacc ttagaggcta
     9241 tttaagttgc tgatttatat taattttatt gttcaaacat gagagcttag tacgtgaaac
     9301 atgagagctt agtacgttag ccatgagagc ttagtacgtt agccatgagg gtttagttcg
     9361 ttaaacatga gagcttagta cgttaaacat gagagcttag tacgtgaaac atgagagctt
     9421 agtacgtact atcaacaggt tgaactgctg atcttcagat cctctacgcc ggacgcatcg
     9481 tggccggatc tggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     9541 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     9601 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     9661 ttccagtttg agatctggga atcatttgaa ggttggtact atataaaaat aatatgcatt
     9721 taatactagc gacgccatct atgtgtcaga ccggggactt atcatccaac ctgttagcag
     9781 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9841 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9901 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9961 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatccatcac
    10021 actggcggcc gctcgagcat gcatctagta acggccgcca gtgtgctgga attctgcaga
    10081 tccggtctaa caaagaaaaa cacatttttt tgtgaaaatt cgtttttatt attcaacata
    10141 gttcccttca agagcgatac aacgattata acgaccttcc aattttttga taccattttg
    10201 gtagtactcc ttcggttttg cctcaaaata ggcctcagtt tcggcgatca cctcttcatt
    10261 gcagccaaat tttttccctg cgagcatcct tttgaggtct gagaacaaga aaaagtcgct
    10321 gggggccaga tctggagaat acggcgggtg gggaagcaat tcgaagccca attcatgaat
    10381 ttttgccatc gttctcaatg acttgtggca cggtgcgttg tcttggtgga acaacacttt
    10441 tttcttcttc atgtggggcc gttttgccgc gatttcgacc ttcaaacgct ccaataacgc
    10501 catataatag tcactgttga tggtttttcc cttctcaaga taatcgataa aaattattcc
    10561 atgcgcatcc caaaaaacag aggccattac tttgccagcg gacttttgag tctttccacg
    10621 cttcggagac ggttcaccgg tcgctgtcca ctcagccgac tgtcgattgg actcaggagt
    10681 gtagtgatgg agccatgttt catccattgt cacatatcga cggaaaaact cgggtgtatt
    10741 acgagttaac agctgcaaac accgctcaga atcatcaaca cgttgttgtt tttggtcaaa
    10801 tgtgagctcg cgcggcaccc attttgcaca gagcttccgc atatccaaat attgatgaat
    10861 gatatgacca acacgttcct ttgatatctt taaggcctct gctatctcga tcaacttcat
    10921 tttacggtca ttcaaaatca ttttgtggat ttttttgatg ttttcgtcgg taaccacctc
    10981 tttcgggcgt ccactgcgtt caccgtcctc cgtgctcatt tcaccacgct tgaattttgc
    11041 ataccaatca attattgttg atttccctgg ggcagagtcc ggaaactcat tatcaagcca
    11101 agtttttgct tccactgtat tttttccctt cagaaaacag tattttatca aaacacgaaa
    11161 ttcctttttt tccatatgtg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    11221 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    11281 cattaattgc gttgcgctca cccgctgcat aaccctgctt cggggtcatt atagcgattt
//
