LOCUS       sequence-442477-        9946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. 
            XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows 
            the high background typical of the ZED vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Panara V, Yu H, Peng D, Staxang K, Hodik M, Filipek-Gorniok B, 
            Kazenwadel J, Skoczylas R, Mason E, Allalou A, Harvey NL, Haitina T,
            Hogan BM, Koltowska K
  TITLE     Multiple cis-regulatory elements control prox1a expression in 
            distinct lymphatic vascular beds.
  JOURNAL   Development. 2024 May 1;151(9):dev202525. doi: 10.1242/dev.202525. 
            Epub 2024 May 9.
  PUBMED    38722096
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2024-05-1"; volume: "151(9):dev202525. doi"; 
            pages: " 10.1242/dev.202525. Epub 2024 May 9"
COMMENT     Sequence Label: Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2
FEATURES             Location/Qualifiers
     source          1..9946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(15..26,27..214)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(301..334)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      1012..1021
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1018..1692
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(1198..1218)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1610..1633
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1817..1938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1999..2015)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2610..2630)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2610..2628)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(2633..2666)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    2687..2808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(3018..3731,3732..3734,3735..3737))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3021..3731,3732..3734,3735..3737))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3063..3084)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3412..3431
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3671..3692
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3734..3743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4801..4817)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4820..4853)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4862..4886
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5830..5854)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6446..6479)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(6846..7008,7009..7020)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(7071..7089)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7072..7089)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7107..7123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7107..7123)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7120..7142)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7131..7147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7155..7161,7162..7179,7180..7185))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7200..7221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7338..7355)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7509..8097)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7589..7608)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8268..9059,9060..9128))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8891..8910
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9129..9233)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9311..9766)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9448..9469)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9660..9679
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9892..9914
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9906..9923
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9907..9923
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(9930..9946,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(9930..9946,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccagagg tgtaaagtac ttgagtaatt ttacttgatt actgtactta
       61 agtattattt ttggggattt ttactttact tgagtacaat taaaaatcaa tacttttact
      121 tttacttaat tacatttttt tagaaaaaaa agtacttttt actccttaca attttattta
      181 cagtcaaaaa gtacttattt tttggagatc acttcattct attttccctt gctattacca
      241 aaccaattga attgcgctga tgcccagttt aatttaaata gatctgtcga cttaattaat
      301 gaagttccta tactttctag agaataggaa cttcctcact atagggcgaa ttggagctcc
      361 accgcggtgg cggccgctct agaattctgc acattggctg tttcccaaca gagtgcaagc
      421 tctgagagca aatcttgccc gtcattactg tttgatataa ggtattccca tgcctgacgc
      481 acataatatt tttgttacat tcgtgtttac agcactgaag gcaggcagct atttgtgcag
      541 tcagtacttt atgtatttct aaacccagtg ctggagaggc caataggcag gatgcccccc
      601 ccgcgttagt ccccctgcac aattgtgctg cacctgtcta ctccatttgc agacccctgt
      661 gtctgtgcaa actatttctt tcattgtgct gttttttttg tcacccagca ttacagacat
      721 gcttttttgg gaatccctat ttggccatcc ctagtagtgc tccccccacc tccatacttt
      781 ccatacatgg gctaaggggt ccaaagaccc tgccctcccc cctcacctac tccattaatg
      841 gcttctttgc ttttcaatgg cccagaagct accaaataag ggcaggctgc ctgcctttcc
      901 ctgcggagct cccactgact cctcaactcc aggcagcgta taaattgaca gctcagtccc
      961 caggcagcac ccagccaatt caatcacagc acggggatcg atccaccggt cgccaccatg
     1021 gcctcctccg agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc
     1081 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac
     1141 aacaccgtga agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     1201 tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac
     1261 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1321 ggcggcgtgg cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag
     1381 gtgaagttca tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg
     1441 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc
     1501 cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac
     1561 atggccaaga agcccgtgca gctgcccggc tactactacg tggacgccaa gctggacatc
     1621 acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac
     1681 cacctgttcc tgagatcggc cgcgactcta gatcataatc agccatacca catttgtaga
     1741 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1801 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1921 actcatcaat gtatcttaag atcctagaac tagaactagt ggatcccccg ggctgcagga
     1981 attcgatatc aagcttatcg ataccgtcga cctcgagcgg ccgccagtgt gatggatatc
     2041 tgcagaattc ggcttacaca ctcaagctgg ttctttgaac agagtttaag aaagaagcta
     2101 tttacaatgt tgggggtgga atttaagaag aaaggacagt ggataatgaa aaggatactc
     2161 agaacaactg ctggaacaca gacatcactg tcttgacaaa gtcagctggc aggaactatg
     2221 accttggaga aggatataag caactttctt gttatgtggc aaagaggaag ttgcagacct
     2281 ttgacttcac tctccttctg cctctaaaag ccgaattcca gcacactggc ggccgttact
     2341 agtggatccc ccgggctgca ggaattcgat tatgtcaaaa acctgtactt cataaaattg
     2401 gaaatgacaa ttttcttgat aggtatcact taccaaagtt aaatgaaaat catattaaca
     2461 atttaaatag acctgtaata tataagaaaa taaaagcaat cattaaaaga ctccaaatcc
     2521 cccccggccc cccaaagaaa gcccaaagac tgttttagga cagaattcct gcagcccggg
     2581 ggatccgagc tcggtaccag cttttgttcc ctttagtgag ggttaattgc gtgaagttcc
     2641 tatactttct agagaatagg aacttcttaa ttaaccatgg cgtacgtaag atacattgat
     2701 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2761 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2821 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2881 aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg tactagtagg
     2941 cctattattt ttgacaccag acaagttggt aatggtagcg accggcgctc agttggaatt
     3001 ctagagtcgc ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
     3061 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
     3121 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
     3181 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
     3241 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
     3301 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
     3361 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
     3421 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
     3481 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag
     3541 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
     3601 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
     3661 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
     3721 tcgcccttgc tcaccatggt ggcgaccggt ggatcctcaa gtgtccgcgc ttagaaaatg
     3781 cttggaaaag tttacttgac actacagacc cgaaagcacc actatagtcc gttagattat
     3841 taaaatatct gtcgttagct taatgcttca ggtggcgatg ctctgtttac tttctgagca
     3901 tgcttttatt tttgaaggag tgtagaggtc ctgcttcttt gcattcaagg gtggccaggg
     3961 acacctgtaa gagaataaca gatagaagca tctcagtgtt gacgttaaac acagtgcaag
     4021 acagatctta attcacacga cgacgcctca gttaatataa gtaggctaac ttttgtagtc
     4081 aatcaattaa atcgctcaat taaatgctcg caatttcaaa taactatcgg gaagtactca
     4141 cggcgcaaag ttgcatttca gcactccaat cctgcgttta tttttcccgt gttagtcctg
     4201 cgcaaagaat gttaatcctc aagatgcctg ttgacgttcg taattcgcca ctggtttaaa
     4261 ttgtccacag cccgattgtt aaagtctccc taaaactctg cgtctgtcta aagcggagag
     4321 aagtctcatt aatgaattgt gcctgtgtac tgtcggaact ggcgccagta gattccatat
     4381 cttacgaaca tgggcgggga cacgagctgg cagtgagcgc cctgggaacc aatagcagag
     4441 cgcgctgact gccattggct cggacagcat gctttccgaa atgccacggc gactgtcggc
     4501 ctactgctcc acatccagag agctgagaga ctcatcattc agcgcagcaa tttattgaga
     4561 tctttttacc ctttttagag cttcacgttg atggtgtgta atgatgtctt tttactcaag
     4621 tgctaacaga aaagcctaaa atctaagttg cagcctaatg ctatttttta aatttatttt
     4681 taggacatta aatgatgtga ataatataat tatagacttg caggattata tttaattttt
     4741 aattgtgcag aaatgtatta aaatgcctct attctattaa tgaatatcgc gcaagcttat
     4801 cgataccgtc gacctcgaga taacttcgta tagcatacat tatacgaagt tatcataatc
     4861 aacaagtttg tacaaaaaag caggctgggt agatgtaatt accgtgtaga aacttgtccc
     4921 catgcttgca aatttaagat aatgacgtga atgagaattc atagcgggga atccgagtcg
     4981 ttagaaagag tcagaagcca cgacgggctg ataggtttcc cccaaaaggc cagccgagag
     5041 cataattacc gccagggttc ttctcccata attcagcaga aaatgattaa gtcgtcatcc
     5101 cgaggcatca gtctgcagaa gttcaaacat gtacttaacc tatccaggaa ttatacattt
     5161 ggcctgtgtg tcgcctctgc cttctttcag cgagaaacag ggggtttgaa aagaagacgg
     5221 aaaaaaaaga acaaccaaag aagcaaggct atctgatctc tcgcctgccc ccgtttcaat
     5281 agacgttaaa cctattagga tctactgaac tcgaggggct tctccttttt ttcagcttta
     5341 tctgcggagc gaaatgcact gtccaaattt ccagacactg agataaggac ccccggcccc
     5401 cgtgacgact tactccagcg ccccctcgcc acccccttaa ttcttttaga ataagaaaca
     5461 aaacattaca aaatgatagc agactgctca ctggggaatg aaaattgctt tgacatggag
     5521 attaggctct tctattgtca taagacattg tctcatatga gtggggcttg cagaagagac
     5581 ggataaaagg cgagaaaggg gggaattttt tttctagaat ggctagtgga tgacataatg
     5641 gagagcgcag aaaagtaagt ccgcatgaca tcgtttggca gcgctgtata aagggagatt
     5701 taaaaagcat tgttgccgaa tagaaatacc agagattcgg gggacaatgg agccgcgttg
     5761 ctggaacgtg acaactcagc aattaaggat catgtcatct gcttgtcaaa caaaaataac
     5821 cagcctagca cccagctttc ttgtacaaag tggttgatta tggagttggc gcgcctgtca
     5881 ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc
     5941 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
     6001 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
     6061 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
     6121 ttgggagggg gctgtccccg tgagctccca ggcgcgcctg tcattctaaa tctctctttc
     6181 agcctaaagc tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa
     6241 gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct
     6301 gccggctcgg ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc
     6361 tgccccctag cgggggaggg acgtaattac atccctgggg gctttggggg ggggctgtcc
     6421 ccgtgagctc ccaggcgcgc aactcataac ttcgtatagc atacattata cgaagttatg
     6481 gcccatctgg cctgtgtttc agacaccagg gagtctctgc tcacgtttcc tgctatttgc
     6541 agcctctcta tcaagactaa tacacctctt cccgcatcgg ctgcctgtga gaggcttttc
     6601 agcactgcag gattgctttt cagccccaaa agagctaggc ttgacactaa caattttgag
     6661 aatcagcttc tactgaagtt aaatctgagg ttttacaact ttgagtagcg tgtactggca
     6721 ttagattgtc tgtcttatag tttgataatt aaatacaaac agttctaaag caggataaaa
     6781 ccttgtatgc atttcattta atgttttttg agattaaaag cttaaacaag aatctctagt
     6841 tttctttctt gcttttactt ttacttcctt aatactcaag tacaatttta atggagtact
     6901 tttttacttt tactcaagta agattctagc cagatacttt tacttttaat tgagtaaaat
     6961 tttccctaag tacttgtact ttcacttgag taaaattttt gagtactttt tacacctctg
     7021 ctcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt
     7081 cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7141 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7201 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7261 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7321 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7381 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7441 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7501 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7561 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7621 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7681 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7741 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7801 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7861 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7921 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7981 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8041 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8101 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8161 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8221 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8281 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8341 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8401 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8461 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8521 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8581 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8641 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8701 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8761 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8821 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8881 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8941 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9001 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9061 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9121 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9181 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9241 ccccgaaaag tgccacctga tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     9301 ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     9361 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     9421 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     9481 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     9541 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     9601 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     9661 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     9721 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca
     9781 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     9841 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     9901 gacgttgtaa aacgacggcc agtgaattgt aatacgactc actata
//
