LOCUS       sequence-44251-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pBID-G
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(92..98,99..116,117..122))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(147..271)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    344..774
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    complement(787..820)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
       61 aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
      121 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
      181 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      241 tttctcgttc agcttttttg tacaaacttg tgatatcgag ctctcccggg aattcggatc
      301 cggccctcga gccgcggccg cttaattaag cgaattcaga tctgttgttg gttggcacac
      361 cacaaatata ctgttgccga gcacaattga tcggctaaat ggtatggcaa gaaaaggtat
      421 gcaatataat aatcttttat tgggtatgca acgaaaattt gtttcgtcaa cgtatgcaat
      481 attttttatt aaaagagggt atgcaatgta ttttattaaa aacgggtatg caatataata
      541 atcttttatt gggtatgcaa cgaaaatttg tttcgtcaaa gtatgcaata ttttttatta
      601 aaagagggta tgcaatgtat tttattaaaa acgggtatgc aataaaaaat tatttggttt
      661 ctctaaaaag tatgcagcac ttattttttg ataaggtatg caacaaaatt ttactttgcc
      721 gaaaatatgc aatgtttttg cgaataaatt caacgcacac ttattacgtg gccaggatcc
      781 gctagcataa cttcgtatag catacattat acgaagttat gctagcctag ttccagtgaa
      841 atccaagcat tttctaaa
//
