LOCUS       sequence-442643-        6239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tph-1 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY139
FEATURES             Location/Qualifiers
     source          1..6239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1604..1624
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1646..1666
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3830..3852)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3952..3971
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4049..4067)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4135..4239
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4240..4308,4309..5100)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4458..4477)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5271..5859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5760..5779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6013..6030
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6147..6168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6183..6188,6189..6206,6207..6213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6221..6237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6226..6239,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt atgattccgc
      181 gattggctta tcatcgagtg actcgtaaag aatacatgct cggatcaaga gctcattctc
      241 gcgaattgcg gccgacatat tgaaagaatg aaatataaat tacgtttttt gaataaaaaa
      301 ttttaaaatg gtaactagaa tataaacgtt caaatttaga acacgtatta aacataaatt
      361 tgattgaaaa atttttgaaa tcaaattcaa tttcttcgaa aaacttgaat aaccaaaaaa
      421 aaagtttttg aaaacatttg ttcaaatatt gtagatattg tagaaattgg ttttcacaca
      481 gctttgggta ttcagttttc gtgttccaac aagaaaataa tgaaatcttc aagagatgaa
      541 atttggattt ttacaaaaac acggtctgta acttggctga aaatgaaaat aattaataaa
      601 attgataaaa tattgcaaaa tatttcacag ttactattca acattatttt gctattaaaa
      661 ttcaaaagat gttgatttat gactagtaca tcagaacagt tttcaaaaat tttgatggtt
      721 tttgggagaa tgccttttac tctgtgtaac tcaaaaaata attggcaatt gtttgtaata
      781 aaaagtattt caaaagaaag aaaaattgtt tcttatgatt ttatcagttg atcattcgtt
      841 ttctagacat gtatttttct agatatgcta gtttgaatct acacaattta gtgagtttga
      901 atttagattc cagcgatatg caatgatttt gaagttaatg agtatttgcc tgtttttact
      961 aggtcattcc aaaaatttca actgcagaac tcaacactga accttaaggt aaagcgaaat
     1021 ctgaactttt ttttgctagt ttgagttttt attttaagag atcgaaaaat tttccttgaa
     1081 ctttgaaaca tgacaattca aacttttcaa atgtagtaag ctccgatgcg ttcccgttca
     1141 ttattcttct tcaataaatt cgaaatctga catcattctc atcttttccc atcatcacaa
     1201 gccgtgggct catttattct cccacggaaa ccatgacagc aaaaataaat agagtggcgc
     1261 cttattcgac tcatttcgtt tttttttctc cggatattag attgtgtggc aggcggctcc
     1321 attgtatatt acgtgccgaa ttccagaagc accacgccat cggatatcta aaagaggagg
     1381 tgtctttgtt tgcgcataat aaaacaatca atcaacacag caaagacccc tctcaacctc
     1441 atttcatgat tttctttggt ttttaggtag cattgctctc ttcaatcatc ccgggattgg
     1501 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     1561 gacccttgct tggagggtac cgagctcaga aaaaatgacc gctccaaaga agaagcgcaa
     1621 ggttccagtc gagaagatga ctgccccaaa aaagaaacgt aaggtcccgc ggatgtcaga
     1681 gcttattaag gagaatatgc atatgaaact gtacatggaa ggaaccgtgg acaaccatca
     1741 ctttaagtgc acttcggagg gggaaggcaa accgtacgag ggcacccaga ccatgcgaat
     1801 caaggtggtc gagggaggcc cactgccctt cgccttcgac attctggtaa gtttaaacat
     1861 atatatacta actaaccctg attatttaaa ttttcaggct actagcttcc tgtacggcag
     1921 caagactttt attaatcaca cccagggcat cccggacttc ttcaaacagt cgttccctga
     1981 gggcttcaca tgggagcgtg tgaccacata cgaagacggt ggcgttctca ccgctaccca
     2041 ggacaccagc ctccaggacg gttgcctcat atataatgtg gtaagtttaa acagttcggt
     2101 actaactaac catacatatt taaattttca gaagatccgg ggagtaaact tcacatccaa
     2161 tggtccggtt atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtatcc
     2221 ggctgacgga ggcctggaag gtcgaaacga catggccctg aagctcgtcg gcggtagcca
     2281 tctcatcgca aacgctaaga ccacatatag atccaagaaa ccggtaagtt taaacatgat
     2341 tttactaact aactaatctg atttaaattt tcaggctaag aaccttaaaa tgccgggagt
     2401 ctactatgtt gactacagac tggaacggat caaggaggcc aacaacgaga cctacgtcga
     2461 gcagcacgag gttgcagttg ccagatactg cgacctcccg agcaaactgg gtcacaagct
     2521 taatcctagg ataccggtgg gtgaagaccc accaaagcca tctgccaagg gagccaagaa
     2581 ggccgccaag accgttacga agccaaagga cggaaagaag agacgtcatg cccgtaagga
     2641 atcatactcc gtctacatct accgtgtcct caagcaagtt catccagaca ctggagtttc
     2701 ctccaaagcc atgtctatca tgaactcttt tgtcaacgat gtcttcgagc gtattgctgc
     2761 tgaagcatcc cgtcttgctc actacaacaa gcgttccaca atctcatccc gcgaaattca
     2821 gaccgctgtc cgtctgatcc ttccaggaga gcttgccaag cacgccgtgt ctgagggaac
     2881 caaggccgtt accaagtaca cttccagcaa gtaggaattc caactgagcg ccggtcgcta
     2941 ccattaccaa cttgtctggt gtcaaaaata ataggggccg ctgtcatcag agtaagttta
     3001 aactgagttc tactaactaa cgagtaatat ttaaattttc agcatctcgc gcccgtgcct
     3061 ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc
     3121 accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt
     3181 ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa
     3241 aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca
     3301 caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat
     3361 agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt
     3421 tttatttctt cgcacgtctg ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt
     3481 tttggagtat ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct
     3541 tttgcttttt ggggtttccc ctattgtttg tcaagatttc gaggacggcg tttttcttgc
     3601 taaaatcaca agtattgatg agcacgatgc aagaaagatc ggaagaaggt ttgggtttga
     3661 ggctcagtgg aaggtgagta gaagttgata atttgaaagt ggagtagtgt ctatggggtt
     3721 tttgccttaa atgacagaat acattcccaa tataccaaac ataactgttt cctactagtc
     3781 ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     3841 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     3901 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     3961 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     4021 gaaaataccg catcaggcgg ccttaagggc ctcgtgatac gcctattttt ataggttaat
     4081 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4141 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4201 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4261 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4321 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4381 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4441 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4501 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4561 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4621 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4681 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4741 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4801 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4861 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4921 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4981 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5041 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5101 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5161 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5221 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5281 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5341 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5401 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5461 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5521 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5581 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5641 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5701 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5761 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5821 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5881 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5941 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6001 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6061 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6121 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6181 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaatt
//
