LOCUS       sequence-44290-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Rosonina E, Ip JY, Calarco JA, Bakowski MA, Emili A, McCracken S, 
            Tucker P, Ingles CJ, Blencowe BJ
  TITLE     Role for PSF in mediating transcriptional activator-dependent 
            stimulation of pre-mRNA processing in vivo.
  JOURNAL   Mol Cell Biol. 2005 Aug;25(15):6734-46.
  PUBMED    16024807
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-08"; volume: "25(15)"; pages: "6734-46"
COMMENT     Sequence Label: FLAG-p54
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcgagctc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttagccac catggactac aaagacgatg acgataaagc
      121 aagggatcca atgcagagta ataaaacttt taacttggag aagcaaaacc atactccaag
      181 aaagcatcat caacatcacc accagcagca gcaccaccag cagcaacagc agcagccgcc
      241 accaccgcca atacctgcaa atgggcaaca ggccagcagc caaaatgaag gcttgactat
      301 tgacctgaag aattttagaa aaccaggaga gaagaccttc acccaacgaa gccgtctttt
      361 tgtgggaaat cttcctcccg acatcactga ggaagaaatg aggaaactat ttgagaaata
      421 tggaaaggca ggcgaagtct tcattcataa ggataaagga tttggcttta tccgcttgga
      481 aacccgaacc ctagcggaga ttgccaaagt ggagctggac aatatgccac tccgtggaaa
      541 gcagctgcgt gtgcgctttg cctgccatag tgcatccctt acagttcgaa accttcctca
      601 gtatgtgtcc aacgaactgc tggaagaagc cttttctgtg tttggccagg tagagagggc
      661 tgtagtcatt gtggatgatc gaggaaggcc ctcaggaaaa ggcattgttg agttctcagg
      721 gaagccagct gctcggaaag ctctggacag atgcagtgaa ggctccttcc tgctaaccac
      781 atttcctcgt cctgtgactg tggagcccat ggaccagtta gatgatgaag agggacttcc
//
