LOCUS       sequence-443096-        9781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET-based calcium sensor located in the ER lumen, Tet-inducible.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Crapart CC, Scott ZC, Konno T, Sharma A, Parutto P, Bailey DMD, 
            Westrate LM, Avezov E, Koslover EF
  TITLE     Luminal transport through intact endoplasmic reticulum limits the 
            magnitude of localized Ca(2+) signals.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Mar 26;121(13):e2312172121. doi: 
            10.1073/pnas.2312172121. Epub 2024 Mar 19.
  PUBMED    38502705
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-03-26"; volume: "121(13):e2312172121. 
            doi"; pages: " 10.1073/pnas.2312172121. Epub 2024 Mar 19"
COMMENT     Sequence Label: 
            pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL
FEATURES             Location/Qualifiers
     source          1..9781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             147..327
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    374..499
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    992..1225
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1410..1454
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1725..1841
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1859..1879
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1885..1903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1921..1939
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1957..1975
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1993..2011
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2029..2047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2065..2083
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2101..2119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2164..2188
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2255..2279
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2355..2357,2358..2360,2361..3071)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2355..2357,2358..2360,2361..3071)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3609..3611,3612..3614,3615..4325)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNWNSVNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     ERVTAAGITLGMDELYK"
     protein_bind    complement(4371..4395)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4413..5001
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4466..4486)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4884..4895)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5032..5531
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5067..5090)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5464..5483
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5548..6147
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5548..5567)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6044..6064
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6219..6452
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6524..6645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6561..6580)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6615..6634
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6685..6820
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6840..6859)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6841..6859)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6869..6886)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6869..6885)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6878..6900)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7027..7482
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7114..7133)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7324..7345
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7508..7612
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7613..7681,7682..8473)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7831..7850)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8644..9232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9133..9152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9386..9403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9520..9541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9556..9561,9562..9579,9580..9586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9594..9610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9599..9621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9618..9634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9618..9634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9653..9673
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9655..9673
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(9701..9781,1..146)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
       61 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      121 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      181 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      241 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      301 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      361 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      421 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      481 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      541 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
      601 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
      661 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
      721 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
      781 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
      841 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
      901 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
      961 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1021 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1081 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1141 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1201 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1261 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1321 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1381 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1441 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1501 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1561 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     1621 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     1681 ccattcgatt agtgaacgga tctcgacggt atcgctgagg atccttttaa agaaaagggg
     1741 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     1801 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tactagtgat tatcggatca
     1861 actttgtata gaaaagttgt ttactcccta tcagtgatag agaacgtatg aagagtttac
     1921 tccctatcag tgatagagaa cgtatgcaga ctttactccc tatcagtgat agagaacgta
     1981 taaggagttt actccctatc agtgatagag aacgtatgac cagtttactc cctatcagtg
     2041 atagagaacg tatctacagt ttactcccta tcagtgatag agaacgtata tccagtttac
     2101 tccctatcag tgatagagaa cgtataagct ttaggcgtgt acggtgggcg cctataaaag
     2161 cagagctcgt ttagtgaacc gtcagatcgc ctggagcaat tccacaacac ttttgtctta
     2221 taccaacttt ccgtaccact tcctaccctc gtaaacaagt ttgtacaaaa aagcaggctg
     2281 ccaccatgct gctatccgtg ccgttgctgc tcggcctcct cggcctggcc gtcgccggag
     2341 gttccggcgg aagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     2401 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     2461 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     2521 tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc
     2581 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2641 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     2701 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2761 acatcctggg gcacaagctg gagtacaact acttcagcga caacgtctat atcaccgccg
     2821 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     2881 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2941 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     3001 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     3061 agctgtacaa gatgcatgac caactgacag aagagcagat tgcagagttc aaagaagcct
     3121 tctcattatt cgacaaggat ggggacggca ccatcaccac aaaggaactt ggcaccgtta
     3181 tgaggtcgct tggacaaaac ccaacggaag cagaattgca ggatatgatc aatgaagtcg
     3241 atgctgatgg caatggaacg atttactttc ctgaatttct tactatgatg gctagaaaaa
     3301 tgaaggacac agacagcgaa gaggaaatcc gagaagcatt ccgtgttgct gacaaggatg
     3361 ggaacggcta catcagcgct gctgagttac gtcacgccat gacaaacatc ggggagaagt
     3421 taacagatga agaagttgat gaaatgataa gggaagcaga tatcgatggt gatggccaag
     3481 taaactatga agagtttgta caaatgatga cagcaaaggg ggggaagagg cgctggcaga
     3541 aaacaggcca tgcttggcga gcaataggaa gactgaagaa gatctccagc tccggggcac
     3601 tggagctcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3661 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     3721 ccacctacgg caagctgacc ctgaagctca tctgcaccac cggcaagctg cccgtgccct
     3781 ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc
     3841 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     3901 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     3961 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     4021 tggggcacaa gctggagtac aactggaaca gcgtcaacgt ctatatcatg gccgacaagc
     4081 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     4141 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     4201 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     4261 acatggtcct gctggagaga gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     4321 acaagctgca gtccggaaag gacgagctgt aactcgagat ttccggctaa acccagcttt
     4381 cttgtacaaa gtggtgataa tcgaattccg ataatcaacc tctggattac aaaatttgtg
     4441 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4501 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4561 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4621 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4681 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4741 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4801 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4861 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4921 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4981 ccctttgggc cgcctccccg catcgggaat tcccgcggtt cgaattctac cgggtagggg
     5041 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     5101 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     5161 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     5221 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     5281 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     5341 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     5401 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     5461 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     5521 cgggcctttc gacctcacgt ggccaccatg accgagtaca agcccacggt gcgcctcgcc
     5581 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     5641 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     5701 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     5761 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     5821 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     5881 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     5941 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6001 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6061 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6121 tgcatgaccc gcaagcccgg tgcctgaggt acctttaaga ccaatgactt acaaggcagc
     6181 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6241 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     6301 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6361 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6421 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     6481 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     6541 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6601 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     6661 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6721 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     6781 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     6841 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     6901 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6961 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     7021 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7081 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7141 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7201 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7261 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7321 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7381 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7441 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     7501 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7561 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7621 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7681 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7741 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7801 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7861 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7921 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7981 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8041 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8101 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8161 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8221 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8281 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8341 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8401 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8461 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8521 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8581 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8641 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8701 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8761 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8821 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8881 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8941 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9001 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9061 agagagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     9481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9601 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     9661 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     9721 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     9781 c
//
