LOCUS       sequence-443344-        1758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  phases-of-separation based activity reporter of AMPK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Erdogan YC, Pilic J, Gottschalk B, Yigit EN, Zaki AG, Ozturk G, 
            Eroglu E, Okutan B, Sommer NG, Weinberg AM, Schindl R, Graier WF, 
            Malli R
  TITLE     Development of a Dual Reporter System to Simultaneously Visualize 
            Ca(2+) Signals and AMPK Activity.
  JOURNAL   ACS Sens. 2024 Aug 21. doi: 10.1021/acssensors.4c01058.
  PUBMED    39167044
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Sens. 
            2024 Aug 21. doi: 10.1021/acssensors.4c01058."
COMMENT     Sequence Label: AMPK-SPARK
FEATURES             Location/Qualifiers
     source          1..1758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1063..1116
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gccaccatgg gaggaggggg ctcaggcagc ggagaaggga gcacaaagat gcggagggtg
       61 gccactctcg tggatttggg aaccggcggg tctgagctcg acggagggct ggagtccggc
      121 ttgcgctctg ggtctggtgg tggcgatatt atggtgtcca agggtgagga gctgttcaca
      181 ggcgtcgtgc caatcctggt ggagctggac ggggacgtga atggccacaa attctcagtg
      241 agtggtgagg gggaagggga cgctacatat gggaagctga ccctgaagtt catctgcaca
      301 actggaaaac ttcctgtccc gtggcccaca ctggtgacca cactcaccta cggcgtgcaa
      361 tgtttttcca gatatcccga ccacatgaaa cagcatgact tttttaaatc agctatgccc
      421 gaagggtatg tgcaggagcg cactatcttc tttaaagacg acggtaatta caaaacacga
      481 gccgaagtga agttcgaggg agatactctg gttaacagga ttgaactcaa gggtatcgat
      541 ttcaaagaag atgggaacat attggggcat aagcttgagt ataattacaa ttctcataac
      601 gtctacataa tggccgacaa acaaaagaac ggcatcaagg caaactttaa aattcggcac
      661 aacattgaag atgggtctgt acagctggcc gatcattacc aacagaatac ccccatcggc
      721 gacggtcctg ttctgcttcc ggacaaccac tacttgagca cccagagtgc attgtctaag
      781 gatccaaacg aaaaaaggga ccacatggtg ctgctggagt ttgtaacggc ggccgggatc
      841 accctgggca tggatgagct gtacaagggc agcggtagcg cgggcggatc cgctggggga
      901 agtgccggtg gaagtgcagg tggaagcgct ggaggctctg caggaggctc cgccggagga
      961 tccaggggcg agatcgctaa atccctgaag gaaattgcta aaagtctgaa agaaatagcc
     1021 tggagtctca aggaaatcgc caaatcactg aagggcggaa cggaagggag gggatccctt
     1081 cttacatgtg gcgacgtcga agagaatcca ggcccaaaac tggcgaccat gcacaagttc
     1141 agtcaggagc agattggcga gaacatcgtc tgccgcgtta tttgcaccac cggacagatc
     1201 cctattagag acctttccgc cgacatctca caggtcttga aagagaaacg gtctataaag
     1261 aaggtttgga cttttggacg aaaccctgca tgcgattatc atctgggcaa cattagcaga
     1321 ctctcaaaca agcacttcca gatactcctg ggcgaggatg gcaatcttct gctgaatgac
     1381 attagcacta acgggacttg gctcaatggc caaaaggtgg agaagaacag taatcagctg
     1441 ctcagccagg gcgatgagat tacggtggga gttggagtag agtccgatat tctcagcctg
     1501 gttatcttta ttaatgataa gttcaagcag tgtctggagc aaaataaggt cgatcgaggc
     1561 tcaggctctg ccgggggatc tgctggaggg agcgctgggg gcagtgctgg tgggtctgca
     1621 ggcggttccg caggtggttc cgccgggggc agcagaaccc tgcgggagat cgaagaactt
     1681 ttgcgcaaga tcatcgagga cagcgtaaga tcagtggccg aactcgaaga catcgagaaa
     1741 tggctgaaaa agatatga
//
