LOCUS       sequence-44348-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mid2(SS/TM), containing only the signal sequence and 
            transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity 
            epitope)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS240
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(342..370)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gagcggccgc actagtggat cccccgggct gcaggaattc gatatcaagc ttatcgatac
       61 cgtcgacaat gttgtctttc acaaccaaga atagtttccg cttattactt ttaatactgt
      121 catgcatatc gacgatacgc gcacaatttt tcgtgcaatc atcatcttcg aattcgggtc
      181 tttctaaaaa aaatagaaac atcgtcattg gttgtgtggt tggtattggt gtaccactga
      241 ttctggtgat actggcttta atttacatgt tttgtatcca atcatcaagg acggacgcgt
      301 cgacctcgac aatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      361 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      421 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      481 ggccaacact tgtcactacc ttcacttatg gtgttcaatg cttttcaaga tacccagatc
      541 atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      601 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
      661 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      721 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
//
