LOCUS       sequence-44358-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6642 MSCV-P C-FlagHA 6bE7-Kozak
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     72..94
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     110..126
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     154..173
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        154..172
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    202..226
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(521..545)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             577..600
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             616..642
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             616..642
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(690..713)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cgttgccctc tgggtagccc tttgtcaccc taagcctccg cctcctcttc ctccatccgc
       61 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
      121 cctcactcct tctctaggcg ccggaattag atctaatacg actcactata gggagaccca
      181 agctggctag ttaagctatc aacaagtttg tacaaaaaag ttggcaccat gcatggaaga
      241 catgttaccc taaaggatat tgtattagac ctgcaacctc cagaccctgt agggttacat
      301 tgctatgagc aattagtaga cagctcagaa gatgaggtgg acgaagtgga cggacaagat
      361 tcacaacctt taaaacaaca tttccaaata gtgacctgtt gctgtggatg tgacagcaac
      421 gttcgactgg ttgtgcagtg tacagaaaca gacatcagag aagtgcaaca gcttctgttg
      481 ggaacactaa acatagtgtg tcccatctgc gcaccgaaga ccccaacttt cttgtacaaa
      541 gtggttgatc tagagggccc gcggttcgaa ctcgaggact acaaggacga cgatgacaag
      601 tcggccgctg gaggataccc ctacgacgtg cccgactacg cctagaattc taccgggtag
      661 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
      721 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
      781 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
      841 tcccccccgc ccc
//
