LOCUS       sequence-443761-        8176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split intein version of Trojan-Gal4 Expression Module in Phase 0. 
            Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be 
            converted by cassette exchange into other Trojan exons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Diao F, Vasudevan D, Heckscher ES, White BH
  TITLE     Hox gene-specific cellular targeting using split intein Trojan 
            exons.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Apr 23;121(17):e2317083121. doi: 
            10.1073/pnas.2317083121. Epub 2024 Apr 11.
  PUBMED    38602904
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-04-23"; volume: "121(17):e2317083121. 
            doi"; pages: " 10.1073/pnas.2317083121. Epub 2024 Apr 11"
COMMENT     Sequence Label: psiT-GEM(0)
FEATURES             Location/Qualifiers
     source          1..8176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          675..740
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             870..890
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1162..1296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1199..1218)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1253..1272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    1304..1337
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    1338..1437
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     primer_bind     complement(1468..1488)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1468..1486)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1507..1523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1507..1523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1520..1542)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1531..1547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1555..1561,1562..1579,1580..1585))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1600..1621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1738..1755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1909..2497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1989..2008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2668..3459,3460..3528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3291..3310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3529..3633)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3660..4115
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3747..3766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3957..3978
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4242..4264
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4256..4273
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4257..4273
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4280..4299
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4280..4298
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4327..4426
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             4842..4895
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4896..7538
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             7539..7592
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    7831..7864
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tgaaatagaa ggcgaaggag aggggaggcc atacgaaggc cacaataccg taaagcttaa
       61 ggtaaccaag gggggacctt tgccatttgc ttgggatatt ttgtcaccac aatttcagta
      121 tggaagcaag gtatatgtca agcaccctgc cgacatacca gactataaaa agctgtcatt
      181 tcctgaagga tttaaatggg aaagggtcat gaactttgaa gacggtggcg tcgttactgt
      241 aacccaggat tccagtttgc aggatggctg tttcatctac aaggtcaagt tcattggcgt
      301 gaactttcct tccgatggac ctgttatgca aaagaagaca atgggctggg aagccagcac
      361 tgagcgtttg tatcctcgtg atggcgtgtt gaaaggagag attcataagg ctctgaagct
      421 gaaagacggt ggtcattacc tagttgagtt caaaagtatt tacatggcaa agaagcctgt
      481 gcagctacca gggtactact atgttgactc caaactggat ataacaagcc acaacgaaga
      541 ctatacaatc gttgagcagt atgaaagaac cgagggacgc caccatctgt tcctttagga
      601 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
      661 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
      721 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
      781 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
      841 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
      901 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
      961 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     1021 tggaaaaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     1081 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     1141 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     1201 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1261 tccaaactca tcaatgtatc ttatcatgtc tggatcgcta gcagaagttc ctattctcta
     1321 gaaagtatag gaacttcaac cccttgtgtc atgtcggcga ccctacgccc ccaactgaga
     1381 gaactcaaag gttaccccag ttggggcact actcccgaaa accgcttctg acctgggggc
     1441 gcgccggtac cactagtggg gaggttccct ttagtgaggg ttaattgcgc gcttggcgta
     1501 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     1561 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     1621 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     1681 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1741 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1801 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1861 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1921 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1981 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2041 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2101 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2161 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2221 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2281 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2341 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2401 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2461 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2521 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2581 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2641 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2701 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2761 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2821 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2881 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2941 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3001 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3061 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3121 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3181 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3241 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3301 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3361 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3421 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3481 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3541 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3601 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3661 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3721 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3781 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3841 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     3901 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3961 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4021 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4081 cgcgaatttt aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa
     4141 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     4201 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     4261 aacgacggcc agtgaattgt aatacgactc actatagggc gacccaccgg tgcatgcggc
     4321 ggccgcccca ggtcagaagc ggttttcggg agtagtgccc caactggggt aacctttgag
     4381 ttctctcagt tgggggcgta gggtcgccga catgacacaa ggggtttcta gaagtcgatc
     4441 caacatggcg acttgtccca tccccggcat gtttaaatat actaattatt cttgaactaa
     4501 ttttaatcaa ccgatttatc tctcttccgc aggaatattg cctgtcctac gacaccgaga
     4561 tcctgaccgt ggagtacggc ttcctgccca tcggcaagat cgtggaggag cgcatcgagt
     4621 gcaccgtgta caccgtggac aagaacggct tcgtgtacac ccagcccatc gcccagtggc
     4681 acaaccgcgg cgagcaggag gtgttcgagt actgcctgga ggacggctcc atcatccgcg
     4741 ccaccaagga ccacaagttc atgaccaccg acggccagat gctgcccatc gacgagatct
     4801 tcgagcgcgg cctggacctg aagcaggtgg acggcctgcc cgagggccgc ggcagcctgc
     4861 tgacctgcgg cgatgtggag gagaaccccg ggcccatgaa gctactgtct tctatcgaac
     4921 aagcctgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg
     4981 ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc
     5041 tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc
     5101 tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata
     5161 taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag
     5221 atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg
     5281 cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgattg
     5341 actcggcagc tcatcatgat aactccacaa ttccgttgga ttttatgccc agggatgctc
     5401 ttcatggatt tgattggtct gaagaggatg acatgtcgga tggcttgccc ttcctgaaaa
     5461 cggaccccaa caataatggg ttctttggcg acggttctct cttatgtatt cttcgatcta
     5521 ttggctttaa accggaaaat tacacgaact ctaacgttaa caggctcccg accatgatta
     5581 cggatagata cacgttggct tctagatcca caacatcccg tttacttcaa agttatctca
     5641 ataattttca cccctactgc cctatcgtgc actcaccgac gctaatgatg ttgtataata
     5701 accagattga aatcgcgtcg aaggatcaat ggcaaatcct ttttaactgc atattagcca
     5761 ttggagcctg gtgtatagag ggggaatcta ctgatataga tgttttttac tatcaaaatg
     5821 ctaaatctca tttgacgagc aaggtcttcg agtcaggttc cataattttg gtgacagccc
     5881 tacatcttct gtcgcgatat acacagtgga ggcagaaaac aaatactagc tataattttc
     5941 acagcttttc cataagaatg gccatatcat tgggcttgaa tagggacctc ccctcgtcct
     6001 tcagtgatag cagcattctg gaacaaagac gccgaatttg gtggtctgtc tactcttggg
     6061 agatccaatt gtccctgctt tatggtcgat ccatccagct ttctcagaat acaatctcct
     6121 tcccttcttc tgtcgacgat gtgcagcgta ccacaacagg tcccaccata tatcatggca
     6181 tcattgaaac agcaaggctc ttacaagttt tcacaaaaat ctatgaacta gacaaaacag
     6241 taactgcaga aaaaagtcct atatgtgcaa aaaaatgctt gatgatttgt aatgagattg
     6301 aggaggtttc gagacaggca ccaaagtttt tacaaatgga tatttccacc accgctctaa
     6361 ccaatttgtt gaaggaacac ccttggctat cctttacaag attcgaactg aagtggaaac
     6421 agttgtctct tatcatttat gtattaagag attttttcac taattttacc cagaaaaagt
     6481 cacaactaga acaggatcaa aatgatcatc aaagttatga agttaaacga tgctccatca
     6541 tgttaagcga tgcagcacaa agaactgtta tgtctgtaag tagctatatg gacaatcata
     6601 atgtcacccc atattttgcc tggaattgtt cttattactt gttcaatgca gtcctagtac
     6661 ccataaagac tctactctca aactcaaaat cgaatgctga gaataacgag accgcacaat
     6721 tattacaaca aattaacact gttctgatgc tattaaaaaa actggccact tttaaaatcc
     6781 agacttgtga aaaatacatt caagtactgg aagaggtatg tgcgccgttt ctgttatcac
     6841 agtgtgcaat cccattaccg catatcagtt ataacaatag taatggtagc gccattaaaa
     6901 atatcgtcgg ttctgcaact atcgcccaat accctactct tccggaggaa aatgtcaaca
     6961 atatcagtgt taaatatgtt tctcctggct cagtagggcc ttcacctgtg ccattgaaat
     7021 caggagcaag tttcagtgat ctagtcaagc tgttatctaa ccgtccaccc tctcgtaact
     7081 ctccagtgac aataccaaga agcacacctt cgcatcgctc agtcacgcct tttctagggc
     7141 aacagcaaca gctgcaatca ttagtgccac tgaccccgtc tgctttgttt ggtggcgcca
     7201 attttaatca aagtgggaat atcgctgata gctcattgtc cttcactttc actaacagta
     7261 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg
     7321 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg
     7381 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt
     7441 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat
     7501 tcgatgatga agatacccca ccaaacccaa aaaaagagga gggccgcggc agcctgctga
     7561 cctgcggcga tgtggaggag aaccccgggc ccgtgaagat catctcccgc aagtccctgg
     7621 gcacccagaa cgtgtacgac atcggcgtgg agaaggacca caacttcctg ctgaagaacg
     7681 gcctggtggc ctccaactgc ttcaacgagg taagttattg aacaatggca tcaaatgcct
     7741 tcatcatcac taccctttag cccttaagac cccacaatga ccttacccac tcagagaaaa
     7801 aagtaaatat gaaagcccat ttgaacttct gaagttccta ttctctagaa agtataggaa
     7861 cttctcctag gggatctaat tcaattagag actaattcaa ttagagctaa ttcaattagg
     7921 atccaagctt atcgatttcg aaccctcgac cgccggagta taaatagagg cgcttcgtct
     7981 acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc
     8041 aaataaacaa gcgcagctga acaagctaaa caatcggact agagccggtc gccaccatga
     8101 ggtcttccaa gaatgttatc aaggagttca tgaggtttaa ggttcgcatg gaaggaacgg
     8161 tcaatgggca cgagtt
//
