LOCUS       sequence-44379-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: TALE2
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..56)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(78..227)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(252..272)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actgggccag gattctcctc gacgtcaccg catgttagca gacttcctct gccctctcca
       61 ctgcctctag agttaatcag catgtccagg tcgaaatcat caagggcgtc actgccgagc
      121 atgtcgaggt caaagtcatc aagggcatcc gaaccaagca tgtcaaggtc aaaatcatcg
      181 agggcgtcac ttcccagcat atccagatca aaatcgtcca atgcgtcagc ccgtccggaa
      241 ccgctggcct ccacctttct cttcttcttg gggctagctg acgcgcgagt ttggtcccct
      301 tcatgcatgg gcgagggcgc gtccaaatcc ctctccagcg agtctgcgaa cgcatgaagg
      361 ctcgcctggt caggtgtctg agttgacgta ggtgaaggct tcgcgcgttt cataccgctc
      421 gcttggagaa tcctgtccca ccgctgggag gcgggaggca gtgtgcccga gcgggcctcg
      481 agttctgtga caccgactct acgaaagagc tgcagcagtc cgtgtctcga cataccaaat
      541 tgagtcatgg cgtcatcgaa cgcttgtgcg gggtgggagt gacactggaa gaatccgagc
      601 acgcggacca cttgcgcgtg atctgccact cgatgtgatg tcctctcggg aatccttctg
      661 ttggtccgct taatcaatgc aggcgcgtgc gggagcccct tcttcaccgc atccaaggcg
      721 ggtcgtccgc cgaggcaggc cagcgctaca agatgatcat tagtgagtgc ggccagcgcg
      781 gggtccggcc tcgaaagctg ggccacgatt gactccagtg
//
