LOCUS       sequence-443804-        5822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  VSV-G envelope expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5822)
  TITLE     Trono Lab Packaging and Envelope Plasmids
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMD2.G
FEATURES             Location/Qualifiers
     source          1..5822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     572..596
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          709..1184
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             join(1259..1306,1307..2794)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    2995..3389
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(3728..3745)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3899..4487)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3979..3998)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4658..5449,5450..5518))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5281..5300
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5519..5623)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5693..5712)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        join(5814..5822,1..371)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
      601 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
      661 cctcgaagct tacatgtggt accgagctcg gatcctgaga acttcagggt gagtctatgg
      721 gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca taggaagggg
      781 agaagtaaca gggtacacat attgaccaaa tcagggtaat tttgcatttg taattttaaa
      841 aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac tttccctaat
      901 ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc attctaaaga
      961 ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata aatatttctg
     1021 catataaatt gtaactgatg taagaggttt catattgcta atagcagcta caatccagct
     1081 accattctgc ttttatttta tggttgggat aaggctggat tattctgagt ccaagctagg
     1141 cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg ggcaacgtgc
     1201 tggtctgtgt gctggcccat cactttggca aagcacgtga gatctgaatt ctgacactat
     1261 gaagtgcctt ttgtacttag cctttttatt cattggggtg aattgcaagt tcaccatagt
     1321 ttttccacac aaccaaaaag gaaactggaa aaatgttcct tctaattacc attattgccc
     1381 gtcaagctca gatttaaatt ggcataatga cttaataggc acagccttac aagtcaaaat
     1441 gcccaagagt cacaaggcta ttcaagcaga cggttggatg tgtcatgctt ccaaatgggt
     1501 cactacttgt gatttccgct ggtatggacc gaagtatata acacattcca tccgatcctt
     1561 cactccatct gtagaacaat gcaaggaaag cattgaacaa acgaaacaag gaacttggct
     1621 gaatccaggc ttccctcctc aaagttgtgg atatgcaact gtgacggatg ccgaagcagt
     1681 gattgtccag gtgactcctc accatgtgct ggttgatgaa tacacaggag aatgggttga
     1741 ttcacagttc atcaacggaa aatgcagcaa ttacatatgc cccactgtcc ataactctac
     1801 aacctggcat tctgactata aggtcaaagg gctatgtgat tctaacctca tttccatgga
     1861 catcaccttc ttctcagagg acggagagct atcatccctg ggaaaggagg gcacagggtt
     1921 cagaagtaac tactttgctt atgaaactgg aggcaaggcc tgcaaaatgc aatactgcaa
     1981 gcattgggga gtcagactcc catcaggtgt ctggttcgag atggctgata aggatctctt
     2041 tgctgcagcc agattccctg aatgcccaga agggtcaagt atctctgctc catctcagac
     2101 ctcagtggat gtaagtctaa ttcaggacgt tgagaggatc ttggattatt ccctctgcca
     2161 agaaacctgg agcaaaatca gagcgggtct tccaatctct ccagtggatc tcagctatct
     2221 tgctcctaaa aacccaggaa ccggtcctgc tttcaccata atcaatggta ccctaaaata
     2281 ctttgagacc agatacatca gagtcgatat tgctgctcca atcctctcaa gaatggtcgg
     2341 aatgatcagt ggaactacca cagaaaggga actgtgggat gactgggcac catatgaaga
     2401 cgtggaaatt ggacccaatg gagttctgag gaccagttca ggatataagt ttcctttata
     2461 catgattgga catggtatgt tggactccga tcttcatctt agctcaaagg ctcaggtgtt
     2521 cgaacatcct cacattcaag acgctgcttc gcaacttcct gatgatgaga gtttattttt
     2581 tggtgatact gggctatcca aaaatccaat cgagcttgta gaaggttggt tcagtagttg
     2641 gaaaagctct attgcctctt ttttctttat catagggtta atcattggac tattcttggt
     2701 tctccgagtt ggtatccatc tttgcattaa attaaagcac accaagaaaa gacagattta
     2761 tacagacata gagatgaacc gacttggaaa gtaactcaaa tcctgcacaa cagattcttc
     2821 atgtttggac caaatcaact tgtgatacca tgctcaaaga ggcctcaatt atatttgagt
     2881 ttttaatttt tatgaaaaaa aaaaaaaaaa acggaattca ccccaccagt gcaggctgcc
     2941 tatcagaaag tggtggctgg tgtggctaat gccctggccc acaagtatca ctaagctcgc
     3001 tttcttgctg tccaatttct attaaaggtt cctttgttcc ctaagtccaa ctactaaact
     3061 gggggatatt atgaagggcc ttgagcatct ggattctgcc taataaaaaa catttatttt
     3121 cattgcaatg atgtatttaa attatttctg aatattttac taaaaaggga atgtgggagg
     3181 tcagtgcatt taaaacataa agaaatgaag agctagttca aaccttggga aaatacacta
     3241 tatcttaaac tccatgaaag aaggtgaggc tgcaaacagc taatgcacat tggcaacagc
     3301 ccctgatgcc tatgccttat tcatccctca gaaaaggatt caagtagagg cttgatttgg
     3361 aggttaaagt tttgctatgc tgtattttac attacttatt gttttagctg tcctcatgaa
     3421 tgtcttttca ctacccattt gcttatcctg catctctcag ccttgactcc actcagttct
     3481 cttgcttaga gataccacct ttcccctgaa gtgttccttc catgttttac ggcgagatgg
     3541 tttctcctcg cctggccact cagccttagt tgtctctgtt gtcttataga ggtctacttg
     3601 aagaaggaaa aacagggggc atggtttgac tgtcctgtga gcccttcttc cctgcctccc
     3661 ccactcacag tgacccggaa tccctcgaca tggcagtcta gcactagtgc ggccgcagat
     3721 ctgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3841 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3901 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3961 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4021 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4081 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4141 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4201 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4261 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4321 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4381 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4441 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4501 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4561 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4621 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4681 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     4741 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     4801 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4861 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4921 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4981 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5041 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5101 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5161 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5221 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5281 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5341 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5401 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5461 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5521 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5581 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     5641 tgccacctga cgtggatccc ctgagggggc ccccatgggc tagaggatcc ggcctcggcc
     5701 tctgcataaa taaaaaaaat tagtcagcca tgagcttggc ccattgcata cgttgtatcc
     5761 atatcataat atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg
     5821 at
//
