LOCUS       sequence-444119-        9482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9482)
  AUTHORS   Ariumi Y, Turelli P, Masutani M, Trono D
  TITLE     DNA damage sensors ATM, ATR, DNA-PKcs, and PARP-1 are dispensable 
            for human immunodeficiency virus type 1 integration.
  JOURNAL   J Virol. 2005 Mar . 79(5):2973-8.
  PUBMED    15709017
REFERENCE   2  (bases 1 to 9482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2005 Mar . 79(5):2973-8."
COMMENT     Sequence Label: pLV.ATRi
FEATURES             Location/Qualifiers
     source          1..9482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..215
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     136..155
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        311..640
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      491..626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             650..1249
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(650..669)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1146..1166
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1320..1908
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1373..1393)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1791..1802)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2001..2234
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2259..2277)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2260..2277)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2567..2586)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2800..2819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2919..2941
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2979..2997)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3065..3169
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3170..3238,3239..4030)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3388..3407)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4201..4789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4690..4709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4943..4960
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        5035..5364
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5215..5350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6498..6563
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6693..6713
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7138..7272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7175..7194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7229..7248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7440..8073
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8120..8245
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8738..8971
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9156..9200
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaacgctgac gtcatcaacc cgctccaagg aatcgcgggc ccagtgtcac taggcgggaa
       61 cacccagcgc gcgtgcgccc tggcaggaag atggctgtga gggacagggg agtggcgccc
      121 tgcaatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga aatgtctttg
      181 gatttgggaa tcttataagt tctgtatgag accacagatc cccggcgtcg tctcagctcg
      241 tcttcaagag agacgagctg agacgacgcc tttttggaaa agcttatcga taccgtcgac
      301 cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
      361 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
      421 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
      481 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
      541 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
      601 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttacca tgaccgagta
      661 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
      721 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
      781 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
      841 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
      901 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
      961 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     1021 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     1081 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     1141 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     1201 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
     1261 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgaatt aattccgata
     1321 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     1381 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     1441 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     1501 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     1561 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     1621 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     1681 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     1741 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     1801 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     1861 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggaattaa
     1921 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     1981 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     2041 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     2101 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     2161 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     2221 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     2281 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     2341 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     2401 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     2461 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     2521 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     2581 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     2641 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     2701 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     2761 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
     2821 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2881 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2941 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     3001 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     3061 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3121 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3181 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3241 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3301 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3361 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3421 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3481 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3541 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3601 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3661 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3721 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3781 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3841 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3901 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3961 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4021 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4081 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4141 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4201 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4261 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4321 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4381 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4441 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4501 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4801 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4861 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4921 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     4981 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
     5041 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5101 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5161 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5281 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5341 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     5401 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     5461 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     5521 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     5581 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     5641 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     5701 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     5761 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     5821 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     5881 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     5941 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     6001 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     6061 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     6121 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     6181 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     6241 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     6301 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     6361 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     6421 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     6481 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     6541 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     6601 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     6661 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     6721 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     6781 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     6841 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     6901 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     6961 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     7021 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     7081 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     7141 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7201 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7261 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     7321 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     7381 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     7441 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     7501 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     7561 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     7621 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     7681 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     7741 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     7801 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     7861 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     7921 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7981 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8041 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     8101 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     8161 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     8221 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     8281 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     8341 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     8401 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     8461 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     8521 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     8581 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     8641 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     8701 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     8761 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     8821 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     8881 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     8941 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     9001 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     9061 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     9121 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     9181 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     9241 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     9301 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     9361 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     9421 acagatccat tcgattagtg aacggatctc gacggtatcg atcccccggg ctgcaggaat
     9481 tc
//
