LOCUS       sequence-444155-       10471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human RNF149 with 3x myc tag in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Kim J, de Haro M, Al-Ramahi I, Garaicoechea LL, Jeong HH, Sonn JY, 
            Tadros B, Liu Z, Botas J, Zoghbi HY
  TITLE     Evolutionarily conserved regulators of tau identify targets for new 
            therapies.
  JOURNAL   Neuron. 2023 Mar 15;111(6):824-838.e7. doi: 
            10.1016/j.neuron.2022.12.012. Epub 2023 Jan 6.
  PUBMED    36610398
REFERENCE   2  (bases 1 to 10471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2022.12.012"; journalName: "Neuron"; date: 
            "2023-03-15- 15"; volume: "111"; issue: "6"; pages: "824-838.e7"
COMMENT     Sequence Label: Human RNF149-3X MYC
FEATURES             Location/Qualifiers
     source          1..10471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1184..1318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1221..1240)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1275..1294
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1486..2119
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2166..2291
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2788..3021
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3206..3250
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3513..3628
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        3686..3989
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3990..4193
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4144..4164
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4190..4214
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4348..4372
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5579..5603)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5611..5700
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     misc_feature    5729..6301
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5896..5913)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6123..6142
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6307..6327
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6328..7020
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="ESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMK
                     STKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLH
                     VSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRT
                     FSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAFK
                     TPDADAGEE"
     misc_feature    7570..8158
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7623..7643)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8041..8052)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8365..8598
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8699..8716)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8705..8929
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8975..9403
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9062..9081)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9272..9293
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9412..9432)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9417..9746
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9597..9732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9659..9678
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     rep_origin      complement(9883..10471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9963..9982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
     1201 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1261 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     1321 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     1381 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     1441 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
     1501 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     1561 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     1621 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     1681 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     1741 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     1801 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     1861 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     1921 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     1981 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     2041 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     2101 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     2161 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     2221 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     2281 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     2341 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     2401 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     2461 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     2521 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     2581 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     2641 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     2701 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     2761 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     2821 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     2881 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     2941 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     3001 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     3061 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     3121 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     3181 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     3241 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     3301 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     3361 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     3421 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     3481 tcgattagtg aacggatctc gacggtatcg cctttaaaag aaaagggggg attggggggt
     3541 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     3601 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     3661 tttatcgata agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     3721 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     3781 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     3841 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     3901 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     3961 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     4021 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     4081 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     4141 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     4201 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     4261 gactctacta gattaattaa gtttaaactt aagcttggta ccgagctcgg atccactagt
     4321 ccagtgtggt ggaattctgc agatatcaca agtttgtaca aaaaagctgg caccatggcg
     4381 tggcggcggc gcgaagccag cgtcggggct cgcggcgtgt tggctctggc gttgctcgcc
     4441 ctggccctgt gcgtgcccgg ggcccggggc cgggctctcg agtggttctc ggccgtggta
     4501 aacatcgagt acgtggaccc gcagaccaac ctgacggtgt ggagcgtctc ggagagtggc
     4561 cgcttcggcg acagctcgcc caaggagggc gcgcatggcc tggtgggcgt cccgtgggcg
     4621 cccggcggag acctcgaggg ctgcgcgccc gacacgcgct tcttcgtgcc cgagcccggc
     4681 ggccgagggg ccgcgccctg ggtcgccctg gtggctcgtg ggggctgcac cttcaaggac
     4741 aaggtgctgg tggcggcgcg gaggaacgcc tcggccgtcg tcctctacaa tgaggagcgc
     4801 tacgggaaca tcaccttgcc catgtctcac gcgggaacag gaaatatagt ggtcattatg
     4861 attagctatc caaaaggaag agaaattttg gagctggtgc aaaaaggaat tccagtaacg
     4921 atgaccatag gggttggcac ccggcatgta caggagttca tcagcggtca gtctgtggtg
     4981 tttgtggcca ttgccttcat caccatgatg attatctcgt tagcctggct aatattttac
     5041 tatatacagc gtttcctata tactggctct cagattggaa gtcagagcca tagaaaagaa
     5101 actaagaaag ttattggcca gcttctactt catactgtaa agcatggaga aaagggaatt
     5161 gatgttgatg ctgaaaattg tgcagtgtgt attgaaaatt tcaaagtaaa ggatattatt
     5221 agaattctgc catgcaagca tatttttcat agaatatgca ttgacccatg gcttttggat
     5281 caccgaacat gtccaatgtg taaacttgat gtcatcaaag ccctaggata ttggggagag
     5341 cctggggatg tacaggagat gcctgctcca gaatctcctc ctggaaggga tccagctgca
     5401 aatttgagtc tagctttacc agatgatgac ggaagtgatg agagcagtcc accatcagcc
     5461 tcccctgctg aatctgagcc acagtgtgat cccagcttta aaggagatgc aggagaaaat
     5521 acggcattgc tagaagccgg caggagtgac tctcggcatg gcggacccat ctccggcgac
     5581 ccagctttct tgtacaaagt ggttgctagc gagcagaaac tcatctctga agaagatctg
     5641 gaacaaaagt tgatttcaga agaagatctg gaacagaagc tcatctctga ggaagatctg
     5701 taagctagct aatgaaccgg gcgcgccgcc cctctccctc ccccccccct aacgttactg
     5761 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     5821 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     5881 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     5941 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     6001 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     6061 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     6121 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     6181 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     6241 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat
     6301 accatgccca agaagaagag aaaggtggag agcgacgaga gcggcctgcc cgccatggag
     6361 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     6421 gagggcaccc ccgagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     6481 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     6541 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     6601 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     6661 gaggccggcc gcgtgatcgg cgacttcaag gtgatgggca ccggcttccc cgaggacagc
     6721 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     6781 ggcgataacg atctggatgg cagcttcacc cgcaccttca gcctgcgcga cggcggctac
     6841 tacagctccg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     6901 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg aggatcacag caacaccgag
     6961 ctgggcatcg tggagtacca gcacgccttc aagaccccgg atgcagatgc cggtgaagaa
     7021 agatctcgag ctcaagcttc gaattctgca gtcgagcagc tgttgaattt tgaccttctt
     7081 aagcttgcgg gagacgtcga gtccaaccct gggcccggta ccaggagggc caccatgaag
     7141 accttcaaca tctctcagca ggatctggag ctggtggagg tcgccactga gaagatcacc
     7201 atgctctatg aggacaacaa gcaccatgtc ggggcggcca tcaggaccaa gactggggag
     7261 atcatctctg ctgtccacat tgaggcctac attggcaggg tcactgtctg tgctgaagcc
     7321 attgccattg ggtctgctgt gagcaacggg cagaaggact ttgacaccat tgtggctgtc
     7381 aggcacccct actctgatga ggtggacaga tccatcaggg tggtcagccc ctgtggcatg
     7441 tgcagagagc tgatctctga ctatgctcct gactgctttg tgctcattga gatgaatggc
     7501 aagctggtca aaaccaccat tgaggaactc atccccctca agtacaccag gaactaataa
     7561 taagttaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7621 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7681 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7741 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7801 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7861 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7921 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     7981 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     8041 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     8101 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     8161 ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     8221 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     8281 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8341 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagatc
     8401 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8461 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8521 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8581 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     8641 caaagaaatg aatatcagag agtgagaggc cttgacattg tttaaacccg ctgatcagcc
     8701 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     8761 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     8821 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     8881 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     8941 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     9001 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     9061 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9121 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9181 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9241 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9301 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     9361 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     9421 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     9481 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     9541 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     9601 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     9661 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     9721 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     9781 ctgatcagca cgtgatgaaa aagcctgaac tcaccgcgac gtcatgtgag caaaaggcca
     9841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10441 gcagattacg cgcagaaaaa aaggatctca a
//
