LOCUS       sequence-444250-        4740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication
            origin), R6K (E. coli replication origin) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Feng J, Qian Y, Zhou Z, Ertmer S, Vivas EI, Lan F, Hamilton JJ, Rey 
            FE, Anantharaman K, Venturelli OS
  TITLE     Polysaccharide utilization loci in Bacteroides determine population 
            fitness and community-level interactions.
  JOURNAL   Cell Host Microbe. 2022 Feb 9;30(2):200-215.e12. doi: 
            10.1016/j.chom.2021.12.006. Epub 2022 Jan 6.
  PUBMED    34995484
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2021.12.006"; journalName: "Cell Host Microbe"; 
            date: "2022-02-9- 9"; volume: "30"; issue: "2"; pages: "200-215.e12"
COMMENT     Sequence Label: pFW1000
FEATURES             Location/Qualifiers
     source          1..4740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            102..211
                     /note="incP origin of transfer"
     rep_origin      382..770
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     primer_bind     803..819
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     803..819
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(3851..3919,3920..4711)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4069..4088)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caattcgagg gggatcaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
       61 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
      121 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
      181 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
      241 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
      301 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaacggaa ttgatccggc
      361 cacgatgcgt ccggcgtaga ggatctgaag atcagcagtt caacctgttg atagtacgta
      421 ctaagctctc atgtttcacg tactaagctc tcatgtttaa cgtactaagc tctcatgttt
      481 aacgaactaa accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc
      541 atgtttcacg tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta
      601 aatagcctct aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc
      661 ttttaaggtt taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta
      721 gagcctctca aagcaatttt gagtgacaca ggaacactta acggctgaca tgggaattcc
      781 cctccaccgc ggtggcggcc gctctagaac tagtggatcc cccgggctgc aggaattcga
      841 tatcaagctt atcgataccg tcgacgtcga ctaattgcct atcttccagt gataactatc
      901 tatgaaactg tttgatactt ttaggtacct acccggagtg tttcagccgg gtaatcttgt
      961 tacttatcct tgattttccc tttaagggcg tttataatcc accctttcgg attgttcttt
     1021 tctcgtgatt ttccgtttag gagagccagt tctccgataa ggtcggttat cttttcttgt
     1081 gccgttatga atgtctcttt gttccggttt atctcttccg atgtgaagcc gcaggaataa
     1141 cggaggtact cgtacacatg gctgtctatc tgatatcgtg ctgtaacctt tgcttgcaat
     1201 tctttccctt ccagttcttc atctctgaac tgtggttgat agaccgggta gaacctaaac
     1261 ccggtcacct tgctacgttt gttgtttggg ttttcacgca ctttcacgta gttgaaagtg
     1321 tatgggcagc tctcatcaag cgcagccttt gctggtttca aaactctttc ctcaaaatga
     1381 tctattctgt cttttccgtt cttgtccttg tatttgtccg caggtatgcc aagacggtct
     1441 ttcaagtttt caagggatat atccaaaggg tacacctgcc cactcattaa catgtaaaac
     1501 ctaagggaat acccagtagg tagcgcaaga gccttgttta actcaaattc tctgtacccc
     1561 ttggcgaact tggtaaacac atcccaaagg tcgttagaca cacgaaaggt tataatgcca
     1621 gtgcgttttt tatattttgg gttagatata aatccgcact tccaccattc ctcgtcatct
     1681 tcgtaagtaa aaaaacgtcc tgccaaagaa tcgagtgcgg ctatgatttc attgtaatca
     1741 cgtccggaaa aaataacgtc agatacatgc atttgtgcat caacatccca aagtccatgc
     1801 tcaaacttgc gtttcgtgcc tgcataatct ttcagtttta ctccctttag ttcagcttgg
     1861 cacgcttcca gtatgcgaag aactattctt tgttcgtgta tggacatgtc ttgctgtttt
     1921 gaccatgtat atacccatga aacaacaaca tctttattct tggtaatagg tagtttcttt
     1981 ttcataaacg attgatttgc tgcaaatata cacaatcccc acattaaaac aaaaaaagag
     2041 gggtaaaact cccaaagtgt ggggactaat ttacttttaa actcccaaag tgtggggact
     2101 aaactcccaa agtgtgggga aacacatgtt ctaatccctt tatttatggg cgtttgcgaa
     2161 gaagtgatag agcatattac agacgatgct tataacagac aaggggaatt tatagagcta
     2221 agtggttgat ttactgtcgg aaaaagaaaa acgataggga aaaagagcaa agcaaaacca
     2281 accaacgggc ggtaggattg gaaacgggtt tttatggcgt gtacacgttt gtgtacaaaa
     2341 tgtttgtatt ttattgaaat atagctatct ttgtgctgaa tttaaaagca aacagatatg
     2401 gaagcattat cagtaagaga ataccgtaac aacctcgcag cgtctttcac caaagctgac
     2461 aatggcgagc aggtactaat tcgcaggaaa aacgagattt acgctttggt aaaagttggt
     2521 cgtgaagatt tgatgataac cccggagctg caagcaagga ttgacaaggc aagggaagaa
     2581 ggtaccgagg acgcgtaaac atttacagtt gcatgtggcc tattgttttt agccgttaaa
     2641 tattttataa ctattaaata gcgatacaaa ttgttcgaaa ctaatattgt ttatatcata
     2701 tattctcgca tgttttaaag ctttattaaa ttgatttttt gtaaacagtt tttcgtactc
     2761 tttgttaacc catttcatta caaaagtttc atattttttt ctctctttaa atgccatttt
     2821 tgctggcttt ctttttaata caattaatgt gctatccact ttaggttttg gatggaaata
     2881 atacctagga atttttgcta atatagaaat atctacctct gccattaaca gcaatgctag
     2941 tgatctgttt gtatctaata acattttagc aaaaccatat tccactatta aataacttat
     3001 tgtggctgaa ctttcaaaaa caatttttcg aattatattt gtgcttatgt tgtaaggtat
     3061 gctgccaaat attttatatg gattgtggct aggaaatgta aatttcagta tatcatcatt
     3121 tactatttga tagttaggat aatttaagag cttattacga gttacctcac ataatttaga
     3181 atcaatttct atcgccgtta caaaattaca tctctttacc aatccagcag taaaatgacc
     3241 tttccctgca cctatttcaa agatgttatc tttttcatct aaacttatgc aattcattat
     3301 tttttctatg tgatattttg aagtaataaa attttgacta tcttttatat ttactttgtt
     3361 cattataacc tctccttaat ttattgcatc tcttttcgaa tatttatgtt ttttgagaaa
     3421 agaacgtact catggttcat cccgatatgc gtatcggtct gtatatcagc aactttctat
     3481 gtgtttcaac tacaatagtc atctattctc atctttctga gtccaccccc tgcaaagccc
     3541 ctctttacga cataaaaatt cggtcggaaa aggtatgcaa aagatgtttc tctctttaag
     3601 agaaactctt cgggatgcaa aaatatgaaa ataactccaa ttcaccaaat tatatagcga
     3661 cttttttaca aaatgctaaa atttgttgat ttccgtcaag caattgttga gcaaaaatgt
     3721 cttttacgat aaaatgatac ctcaatatca actgtttagc aaaacgatat ttctcttaaa
     3781 gagagaaaca cctttttgtt caccaatccc cgacttttaa tcccgcggcc atgattgaaa
     3841 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3901 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3961 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4021 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4081 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4141 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4201 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4261 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4321 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4381 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4441 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4501 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4561 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4621 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4681 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact cataacgcgt
//
