LOCUS       sequence-444254-        6699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication
            origin), p15A (E. coli replication origin), tetQ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Feng J, Qian Y, Zhou Z, Ertmer S, Vivas EI, Lan F, Hamilton JJ, Rey 
            FE, Anantharaman K, Venturelli OS
  TITLE     Polysaccharide utilization loci in Bacteroides determine population 
            fitness and community-level interactions.
  JOURNAL   Cell Host Microbe. 2022 Feb 9;30(2):200-215.e12. doi: 
            10.1016/j.chom.2021.12.006. Epub 2022 Jan 6.
  PUBMED    34995484
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2021.12.006"; journalName: "Cell Host Microbe"; 
            date: "2022-02-9- 9"; volume: "30"; issue: "2"; pages: "200-215.e12"
COMMENT     Sequence Label: pFW5000
FEATURES             Location/Qualifiers
     source          1..6699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            102..211
                     /note="incP origin of transfer"
     rep_origin      276..821
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     722..741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     938..955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     980..996
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     980..996
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5444..5463)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5563..5585
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5623..5641)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5705..5809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5810..5878,5879..6670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6028..6047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caattcgagg gggatcaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
       61 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
      121 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
      181 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
      241 gtctacacga accctttgtt aataagatga tcttcttgag atcgttttgg tctgcgcgta
      301 atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga
      361 gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc
      421 ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc
      481 agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt
      541 accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga
      601 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
      661 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag
      721 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
      781 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc
      841 tctcacttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta
      901 agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc
      961 ggaatatatc cgcggccgct ctagaactag tggatccccc gggctgcagg aattcgatat
     1021 caagcttatc gataccgtcg atgtcgacta attgcctatc ttccagtgat aactatctat
     1081 gaaactgttt gatactttta ggtacctacc cggagtgttt cagccgggta atcttgttac
     1141 ttatccttga ttttcccttt aagggcgttt ataatccacc ctttcggatt gttcttttct
     1201 cgtgattttc cgtttaggag agccagttct ccgataaggt cggttatctt ttcttgtgcc
     1261 gttatgaatg tctctttgtt ccggtttatc tcttccgatg tgaagccgca ggaataacgg
     1321 aggtactcgt acacatggct gtctatctga tatcgtgctg taacctttgc ttgcaattct
     1381 ttcccttcca gttcttcatc tctgaactgt ggttgataga ccgggtagaa cctaaacccg
     1441 gtcaccttgc tacgtttgtt gtttgggttt tcacgcactt tcacgtagtt gaaagtgtat
     1501 gggcagctct catcaagcgc agcctttgct ggtttcaaaa ctctttcctc aaaatgatct
     1561 attctgtctt ttccgttctt gtccttgtat ttgtccgcag gtatgccaag acggtctttc
     1621 aagttttcaa gggatatatc caaagggtac acctgcccac tcattaacat gtaaaaccta
     1681 agggaatacc cagtaggtag cgcaagagcc ttgtttaact caaattctct gtaccccttg
     1741 gcgaactcgg taaacacatc ccaaaggtcg ttagacacac gaaaggttat aatgccagtg
     1801 cgttttttat attttgggtt agatataaat ccgcacttcc accattcctc gtcatcttcg
     1861 taagtaaaaa aacgtcctgc caaagaatcg agtgcggcta tgatttcatt gtaatcacgt
     1921 ccggaaaaaa taacgtcaga tacatgcatt tgtgcatcaa catcccaaag tccatgctca
     1981 aacttgcgtt tcgtgcctgc ataatctttc agttttactc cctttagttc agcttggcac
     2041 gcttccagta tgcgaagaac tattctttgt tcgtgtatgg acatgtcttg ctgttttgac
     2101 catgtatata cccatgaaac aacaacatct ttattcttgg taataggtag tttctttttc
     2161 ataaacgatt gatttgctgc aaatatacac aatccccaca ttaaaacaaa aaaagagggg
     2221 taaaactccc aaagtgtggg gactaattta cttttaaact cccaaagtgt ggggactaaa
     2281 ctcccaaagt gtggggaaac acatgttcta atccctttat ttatgggcgt ttgcgaagaa
     2341 gtgatagagc atattacaga cgatgcttat aacagacaag gggaatttat agagctaagt
     2401 ggttgattta ctgtcggaaa aagaaaaacg atagggaaaa agagcaaagc aaaaccaacc
     2461 aacgggcggt aggattggaa acgggttttt atggcgtgta cacgtttgtg tacaaaatgt
     2521 ttgtatttta ttgaaatata gctatctttg tgctgaattt aaaagcaaac agatatggaa
     2581 gcattatcag taagagaata ccgtaacaac ctcgcagcgt ctttcaccaa agctgacaat
     2641 ggcgagcagg tactaattcg caggaaaaac gagatttacg ctttggtaaa agttggtcgt
     2701 gaagatttga tgataacccc ggagctgcaa gcaaggattg acaaggcaag ggaagaaggt
     2761 accgaggacg cgtaaacatt tacagttgca tgtggcctat tgttttatct ccttaacgta
     2821 cgttttcgtt ccattggccc tcaaaccccg ttatatacat tcatgtccat ttatgtaaaa
     2881 aatcctgctg accttgttta tgtcttgtca gtcaccattt gcaaaaccat atttgaccct
     2941 caaagaggct gaatttgata agcaacttgc tacatactca taataaggag ctaaatagaa
     3001 cacgaatggg aaatactcaa atgccaaact aaagaagata ttggccaaaa taaacgctat
     3061 accgagagag aaacttgatt tttcaacttc ctaaaacagt gttgttcaaa catttctact
     3121 tatttgtact taccagttga acctacgttt ccctaataaa atgtctatgg taaaaagtta
     3181 aaaaatcctc ctacttttgt tagatatatt tttttgtgta attttgtaat cgttatgcgg
     3241 cagtaataat atacatatta atacgagtta ggaatcctgt agttctcata tgctacgagg
     3301 aggtattaaa aggtgcgttt cgacaatgca tctattgtag tatattattg cttaatccaa
     3361 atgaatatta taaatttagg aattcttgct cacattgatg caggaaaaac ttccgtaacc
     3421 gagaatctgc tgtttgccag tggagcaacg gaaaagtgcg gctgtgtgga taatggtgac
     3481 accataacgg actctatgga tatagagaaa cgtagaggaa ttactgttcg ggcttctacg
     3541 acatctatta tctggaatgg tgtgaaatgc aatatcattg acactccggg acacatggat
     3601 tttattgcgg aagtggagcg gacattcaaa atgcttgatg gagcagtcct catcttatcc
     3661 gcaaaggaag gcatacaagc gcagacaaag ttgctgttca atactttaca gaagctgcaa
     3721 atcccgacaa ttatatttat caataagatt gaccgagccg gtgtgaattt ggagcgtttg
     3781 tatctggata taaaagcaaa tctgtctcaa gatgtcctgt ttatgcaaaa tgttgtcgat
     3841 ggatcggttt atccggtttg ctcccaaaca tatataaagg aagaatacaa agaatttgta
     3901 tgcaaccatg acgacaatat attagaacga tatttggcgg atagcgaaat ttcaccggct
     3961 gattattgga atacgataat cgctcttgtg gcaaaagcca aagtctatcc ggtgctacat
     4021 ggatcagcaa tgttcaatat cggtatcaat gagttgttgg acgccatcac ttcttttata
     4081 cttcctccgg catcggtctc aaacagactt tcatcttatc tttataagat agagcatgac
     4141 cccaaaggac ataaaagaag ttttctaaaa ataattgacg gaagtctgag acttcgagac
     4201 gttgtaagaa tcaacgattc ggaaaaattc atcaagatta aaaatctaaa aactatcaat
     4261 cagggcagag agataaatgt tgatgaagtg ggcgccaatg atatcgcgat tgtagaggat
     4321 atggatgatt ttcgaatcgg aaattattta ggtgctgaac cttgtttgat tcaaggatta
     4381 tcgcatcagc atcccgctct caaatcctcc gtccggccag acaggcccga agagagaagc
     4441 aaggtgatat ccgctctgaa tacattgtgg attgaagacc cgtctttgtc cttttccata
     4501 aactcatata gtgatgaatt ggaaatctcg ttatatggtt taacccaaaa ggaaatcata
     4561 cagacattgc tggaagaacg attttccgta aaggtccatt ttgatgagat caagactata
     4621 tacaaagaac gacctgtaaa aaaggtcaat aagattattc agatcgaagt gccgcccaac
     4681 ccttattggg ccacaatagg gctgactctt gaacccttac cgttagggac agggttgcaa
     4741 atcgaaagtg acatctccta tggttatctg aaccattctt ttcaaaatgc cgtttttgaa
     4801 gggattcgta tgtcttgcca atccgggtta catggatggg aagtgactga tctgaaagta
     4861 acttttactc aagccgagta ttatagcccg gtaagtacac ctgctgattt cagacagctg
     4921 accccttatg tcttcaggct ggccttgcaa cagtcaggtg tggacattct cgaaccgatg
     4981 ctctattttg agttgcagat accccaagcg gcaagttcca aagctattac agatttgcaa
     5041 aaaatgatgt ctgagattga agacatcagt tgcaataatg agtggtgtca tattaaaggg
     5101 aaagttccat taaatacaag taaagactat gcatcagaag taagttcata cactaagggc
     5161 ttaggcattt ttatggttaa gccatgcggg tatcaaataa caaaaggcgg ttattctgat
     5221 aatatccgca tgaacgaaaa agataaactt ttattcatgt tccaaaaatc aatgtcatca
     5281 aaataatgga gcggtcagga aatttctata aggcaataca gttgggatat atacttatct
     5341 ccattcttat cggatgtatg gcatataata gcctctatga atggcaggag atagaagcat
     5401 tagaacttgg caataaaaaa atagacgtat ggtgcactct cagtacaatc tgctctgatg
     5461 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     5521 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     5581 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     5641 ttttataggt taatgtcatg ataataatgg tttcttagca ggtggcactt ttcggggaaa
     5701 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5761 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5821 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5881 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5941 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6001 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6061 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6121 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6181 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6241 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6301 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6361 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6421 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6481 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6541 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6601 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6661 gcattggtaa ctgtcagacc aagtttactc ataacgcgt
//
