LOCUS       sequence-444255-       10079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacteroides genomic editing tool; aTc inducible-Fncpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Feng J, Qian Y, Zhou Z, Ertmer S, Vivas EI, Lan F, Hamilton JJ, Rey 
            FE, Anantharaman K, Venturelli OS
  TITLE     Polysaccharide utilization loci in Bacteroides determine population 
            fitness and community-level interactions.
  JOURNAL   Cell Host Microbe. 2022 Feb 9;30(2):200-215.e12. doi: 
            10.1016/j.chom.2021.12.006. Epub 2022 Jan 6.
  PUBMED    34995484
REFERENCE   2  (bases 1 to 10079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2021.12.006"; journalName: "Cell Host Microbe"; 
            date: "2022-02-9- 9"; volume: "30"; issue: "2"; pages: "200-215.e12"
COMMENT     Sequence Label: pFW6000
FEATURES             Location/Qualifiers
     source          1..10079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            102..211
                     /note="incP origin of transfer"
     rep_origin      276..821
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     722..741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     938..955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     980..996
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     980..996
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(998..1083)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1236..1253
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     protein_bind    5225..5243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5287..5305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             5576..6199
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5649..5668)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             join(9190..9258,9259..10050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9408..9427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caattcgagg gggatcaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
       61 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
      121 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
      181 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
      241 gtctacacga accctttgtt aataagatga tcttcttgag atcgttttgg tctgcgcgta
      301 atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga
      361 gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc
      421 ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc
      481 agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt
      541 accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga
      601 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
      661 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag
      721 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
      781 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc
      841 tctcacttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta
      901 agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc
      961 ggaatatatc cgcggccgct ctagaactag tggatccttt gtcctactca ggagagcgtt
     1021 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     1081 ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac
     1141 ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag
     1201 gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa
     1261 tcttctctca tccgccaaaa cagccaagct tttagttgtt acggttctga acgaactcaa
     1321 aatattcttc gtttttaata accagattaa gtttcttacc ctcctggtta tttttaatac
     1381 ggcccaggag cataagtcct ttcagaccaa tgtgataagc tccatttgca tctgcgtctt
     1441 gtggcatatt tttcggcgcc tggcggctgt caaagaagtt cccgttcaca tccgccaccg
     1501 gcgagatcag ataatcgagt tcggtacctg tcttggaatt gcgcatctga aggatagtat
     1561 ttaatacgct ggtgagtttg gcgaaaaact ttttatcgct ttcaccgcaa attgcggctt
     1621 taatacactc accgtgacca tattcgatcg agtagtcttt gagcagtttc tccagctctt
     1681 tcgtaggata cacttcgcgg gtatcccaat tatgattttt gtccgagttg cgaaagttaa
     1741 tcaggcggct gccgaaggag gcaatcgtcc acttgccttt cgcggctttg tcgccgaagt
     1801 ttttgtagtc gaagctgaac tcgaaatagc ccttatccag gttgtagcag attttatcaa
     1861 acttgctaaa gaactcctgg ctcttggata cactttcata ctttgggtac agttggttga
     1921 cgaagcccgt gaccgggcaa atcttgctgg tgaagcctgc cggcacgtaa tagataatac
     1981 ccgtctgttt acccattttc ttgaaggttt cgaacggggc ggtcagctga tacgcacgca
     2041 gaacgccacc tgtcttatca aactcattgt ccttgaagac cagatagttc agtttctcaa
     2101 tcaacatttt ctctaatttc tggtaaacct gtttttcgac cttgaagcga ccgcgtttga
     2161 aaccaaagtt cagatcttca aacactacaa tagcattata ttcaatgact aatttggcga
     2221 tttcgtgtac tacctgagaa agataacctt ctttcatttc cttgatgttg ttgatttttt
     2281 tccagtcttt gcgagcgcta tcacgatctt tctcgatagc agccagtttg tcgtgataat
     2341 tcgttttcat gcgatcatta ccgatgatat tgaaggtatc ctgcttgata atgttaccct
     2401 tgccatccac cagcgtgtaa tacgccagat ggcgttcacc acgatcgatc gacagaatgt
     2461 gcacatcgtt cgctttctct ttcagcagca agttaatttc gtcgttaaat ttgtttgcgc
     2521 cggacgattt aaagttaatc gtgatcgggc agtgaaagaa aaacttatct tccgtaaagc
     2581 gtttgtcttt aatcaggtcg tactcgaaca cgctttcctt tttcggattg tccttgtttt
     2641 tgttggcaat cgcttccttc gccgggtggg taattttctt tggaatgctc tgtttacgat
     2701 aaaacagttc cgcctcgcca ttgagtttgt acactacatc ctggagatta cgctcatcaa
     2761 acaatgcctt ccaatataag gtatggagat tggggcggcc ctttgagtac gcgctgaaat
     2821 ctttattata aatctggaag aggtacagtt tgccctgatt tacaacggag tcaatgtatg
     2881 actcgctgat gttttcgaat gtcagtttat aaccctgatt ttcgacctcg cgatagaact
     2941 catcgatcga gttataacgt tgggtgtcgg agaaacggaa gccaaagtct ttccattcgg
     3001 gatgtttgct gatcgattgt ttgtaaaagt cgataaactt acgacaatct tcaatattga
     3061 actcaaactt ttcatagcct ttttgcgggc tgccattttt ggtatgcgtc gaatgattac
     3121 ggatgcgcag gatgtcttca ctcggattat aaaatttaat gctcttggcg ctaaaaaaca
     3181 ctttcggcag cattttgttc gccccaggca gcagtttata aacgattttc ttgtaacctt
     3241 cacccttatt ttccttgatg gctttgtcgt caaagatttt gttgtttttc ttgttcatga
     3301 cgcctaaata atatttatcg tctttgataa acagaatggc ggtattatct ggctctttat
     3361 tcttatccca accgttcgcc agggtggagt tttcgaaatt cagtttgaat ttttcatcgc
     3421 tatagggttt ctgagtaata taattgcgaa ttttattata gagggggaca atgtttgcta
     3481 attcgaagta gcattcttcg aaaaccaggt agaagtgttc atctttatcg agaatgttcg
     3541 ccttgtcttc tgactggcta atgtgaaaga tcttcagttt atgcagcaga ttattagttt
     3601 ggtccagcag gtctttgatc gctttcacat catcttcggc gctagcctgc aggagatctt
     3661 tcttaccctg attctggtat ttaattgaga tctgcgccag attgtcctta ttctgtgcga
     3721 tttcatcgaa aatcatcgga atggcggcaa aattggccag gatttcttcg aaacggcatt
     3781 gtttgtcgat atcacgatgt ttgttaaatt cttccagggc cagtttaatg gtttccaggc
     3841 tcagatattt cgctttttcc gtttttttcg caatcagttc ctgttctttc ttacttgggt
     3901 tatctaagtt tttcggagca atttgctggg taatatattc cagcacggca gtaccaatca
     3961 cggagtagtc atcaaacacc tgttgggaca ggtcggttaa agacttatcg tttttaaagt
     4021 agattttcga aagatccagc ttctgggctt tgagatcatc aaataagagg ctgagcgtct
     4081 ctttgatcga cttctcttcg acggttttga aagccgcaat ctgttcataa aatgactgca
     4141 tagtcgtcac tacatcggag tcatcttcca gtttgtcaat cacaaatgac ttactttccg
     4201 tatcacttaa aatctgtttg aacagcacgg acatcttata ttttttgagt gttttatcgt
     4261 tgatttgctg gctatacaga ttaatgtatt cgttgatacc tttacgtttg gtgttttcac
     4321 cgttaacgaa tttcccgcca ataatcgtat taaacttagt aatcccggat tgatttaagt
     4381 agttattaaa gtttgcaatc tcgaaaactt cgtccaggct aaagacgcgc tggttcactt
     4441 ccgaggtttt atagtcaata tcaaaagtca actcctccgc caagtccttt ttgatctgtt
     4501 cataattgat cgcttccggc gccttatcct tcagagattc atatttcgct ttgttctcca
     4561 aaaatttggg taaattatcg tcaacgatgc ggtaaatgat agaggttggg atgtcgtttg
     4621 agctgtacac gtttttacga ttttcatgaa atcctttgaa gtaggttgtc caccctttga
     4681 acgatttgat aatttccagt gcttcatcga tatctgtgat gtcgctattt gccttaaaca
     4741 gttcgatgcc gttatctttg gattgtttaa gccacaggat cagatcggat tcctgccctt
     4801 tcttggcatc gatcaggttc tggttgaaca ggtttttgaa cttctcggaa tccttaatgt
     4861 actcactaat ctgtttcttg atagtatcct tggcactctt aaaatctttc tgcagattat
     4921 catcgtccga tttcttcagc ttgaagtaaa catcgctata gttctgaaga aggtcttcac
     4981 tgatacacac ggagctaaga atttcttcaa taaaaaactg atgatatttg tcgatgattt
     5041 gtttagcttt cttgtagtct tttgcacgct tttcgtcatc caggataagg ccacgcgctt
     5101 tgatgttttc cagcgttttg ccttgtggaa ttaattcaaa gcgcagcgtt ttcgacaggg
     5161 aatatttgtt tacgaattcc tgatagatac tcatgtgtct tttcttttat atgtctttat
     5221 ttcgtctcta tcactgatag ggagactgca aagataagaa cttttagctt tatcttccaa
     5281 actttatccc tatcagtgat agagattttt ttgttcttat ggtcaagcag tactgcgctt
     5341 ttacactaag tcaatttaac aaggctttct tctcttggaa agcgggtgca aaaaaagaaa
     5401 aggcgttttg tttttcttct ttaccttctt tccctttcgc taagagagtc tgagaaacga
     5461 tagaaaaaga aaagcgaaaa aacttccgaa aacatttggt agttaaaata aaacctctta
     5521 cctttgcacc cgcgcatttt aaaataaaat aaattattta tgatattaaa cgaatatgtc
     5581 tagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat
     5641 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta
     5701 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag
     5761 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa
     5821 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg
     5881 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca
     5941 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt
     6001 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac
     6061 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc
     6121 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa
     6181 atgtgaaagt gggtcttaat cgatgtcgac taattgccta tcttccagtg ataactatct
     6241 atgaaactgt ttgatacttt taggtaccta cccggagtgt ttcagccggg taatcttgtt
     6301 acttatcctt gattttccct ttaagggcgt ttataatcca ccctttcgga ttgttctttt
     6361 ctcgtgattt tccgtttagg agagccagtt ctccgataag gtcggttatc ttttcttgtg
     6421 ccgttatgaa tgtctctttg ttccggttta tctcttccga tgtgaagccg caggaataac
     6481 ggaggtactc gtacacatgg ctgtctatct gatatcgtgc tgtaaccttt gcttgcaatt
     6541 ctttcccttc cagttcttca tctctgaact gtggttgata gaccgggtag aacctaaacc
     6601 cggtcacctt gctacgtttg ttgtttgggt tttcacgcac tttcacgtag ttgaaagtgt
     6661 atgggcagct ctcatcaagc gcagcctttg ctggtttcaa aactctttcc tcaaaatgat
     6721 ctattctgtc ttttccgttc ttgtccttgt atttgtccgc aggtatgcca agacggtctt
     6781 tcaagttttc aagggatata tccaaagggt acacctgccc actcattaac atgtaaaacc
     6841 taagggaata cccagtaggt agcgcaagag ccttgtttaa ctcaaattct ctgtacccct
     6901 tggcgaactt ggtaaacaca tcccaaaggt cgttagacac acgaaaggtt ataatgccag
     6961 tgcgtttttt atattttggg ttagatataa atccgcactt ccaccattcc tcgtcatctt
     7021 cgtaagtaaa aaaacgtcct gccaaagaat cgagtgcggc tatgatttca ttgtaatcac
     7081 gtccggaaaa aataacgtca gatacatgca tttgtgcatc aacatcccaa agtccatgct
     7141 caaacttgcg tttcgtgcct gcataatctt tcagttttac tccctttagt tcagcttggc
     7201 acgcttccag tatgcgaaga actattcttt gttcgtgtat ggacatgtct tgctgttttg
     7261 accatgtata tacccatgaa acaacaacat ctttattctt ggtaataggt agtttctttt
     7321 tcataaacga ttgatttgct gcaaatatac acaatcccca cattaaaaca aaaaaagagg
     7381 ggtaaaactc ccaaagtgtg gggactaatt tacttttaaa ctcccaaagt gtggggacta
     7441 aactcccaaa gtgtggggaa acacatgttc taatcccttt atttatgggc gtttgcgaag
     7501 aagtgataga gcatattaca gacgatgctt ataacagaca aggggaattt atagagctaa
     7561 gtggttgatt tactgtcgga aaaagaaaaa cgatagggaa aaagagcaaa gcaaaaccaa
     7621 ccaacgggcg gtaggattgg aaacgggttt ttatggcgtg tacacgtttg tgtacaaaat
     7681 gtttgtattt tattgaaata tagctatctt tgtgctgaat ttaaaagcaa acagatatgg
     7741 aagcattatc agtaagagaa taccgtaaca acctcgcagc gtctttcacc aaagctgaca
     7801 atggcgagca ggtactaatt cgcaggaaaa acgagattta cgctttggta aaagttggtc
     7861 gtgaagattt gatgataacc ccggagctgc aagcaaggat tgacaaggca agggaagaag
     7921 gtaccgagga cgcgtaaaca tttacagttg catgtggcct attgttttta gccgttaaat
     7981 attttataac tattaaatag cgatacaaat tgttcgaaac taatattgtt tatatcatat
     8041 attctcgcat gttttaaagc tttattaaat tgattttttg taaacagttt ttcgtactct
     8101 ttgttaaccc atttcattac aaaagtttca tatttttttc tctctttaaa tgccattttt
     8161 gctggctttc tttttaatac aattaatgtg ctatccactt taggttttgg atggaaataa
     8221 tacctaggaa tttttgctaa tatagaaata tctacctctg ccattaacag caatgctagt
     8281 gatctgtttg tatctaataa cattttagca aaaccatatt ccactattaa ataacttatt
     8341 gtggctgaac tttcaaaaac aatttttcga attatatttg tgcttatgtt gtaaggtatg
     8401 ctgccaaata ttttatatgg attgtggcta ggaaatgtaa atttcagtat atcatcattt
     8461 actatttgat agttaggata atttaagagc ttattacgag ttacctcaca taatttagaa
     8521 tcaatttcta tcgccgttac aaaattacat ctctttacca atccagcagt aaaatgacct
     8581 ttccctgcac ctatttcaaa gatgttatct ttttcatcta aacttatgca attcattatt
     8641 ttttctatgt gatattttga agtaataaaa ttttgactat cttttatatt tactttgttc
     8701 attataacct ctccttaatt tattgcatct cttttcgaat atttatgttt tttgagaaaa
     8761 gaacgtactc atggttcatc ccgatatgcg tatcggtctg tatatcagca actttctatg
     8821 tgtttcaact acaatagtca tctattctca tctttctgag tccaccccct gcaaagcccc
     8881 tctttacgac ataaaaattc ggtcggaaaa ggtatgcaaa agatgtttct ctctttaaga
     8941 gaaactcttc gggatgcaaa aatatgaaaa taactccaat tcaccaaatt atatagcgac
     9001 ttttttacaa aatgctaaaa tttgttgatt tccgtcaagc aattgttgag caaaaatgtc
     9061 ttttacgata aaatgatacc tcaatatcaa ctgtttagca aaacgatatt tctcttaaag
     9121 agagaaacac ctttttgttc accaatcccc gacttttaat cccgcggcca tgattgaaaa
     9181 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     9241 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     9301 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9361 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9421 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9481 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9541 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9601 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9661 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9721 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9781 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9841 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9901 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9961 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    10021 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc ataacgcgt
//
