LOCUS       sequence-444308-        5691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of wild-type thyroid receptor beta (THRB, human) with an 
            HA tag in neurons driven by human synapsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Hochbaum DR, Hulshof L, Urke A, Wang W, Dubinsky AC, Farnsworth HC, 
            Hakim R, Lin S, Kleinberg G, Robertson K, Park C, Solberg A, Yang Y,
            Baynard C, Nadaf NM, Beron CC, Girasole AE, Chantranupong L, 
            Cortopassi MD, Prouty S, Geistlinger L, Banks AS, Scanlan TS, Datta 
            SR, Greenberg ME, Boulting GL, Macosko EZ, Sabatini BL
  TITLE     Thyroid hormone remodels cortex to coordinate body-wide metabolism 
            and exploration.
  JOURNAL   Cell. 2024 Oct 3;187(20):5679-5697.e23. doi: 
            10.1016/j.cell.2024.07.041. Epub 2024 Aug 22.
  PUBMED    39178853
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.07.041"; journalName: "Cell"; date: "2024-10-3-
            3"; volume: "187"; issue: "20"; pages: "5679-5697.e23"
COMMENT     Sequence Label: DRH015_ AAV-hSyn-WT-THR
FEATURES             Location/Qualifiers
     source          1..5691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      106..561
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(193..212)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     403..424
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(578..597)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     697..719
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(757..775)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        843..947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(948..1016,1017..1808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1166..1185)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1979..2567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2468..2487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2629..2758
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        2811..3258
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             4686..4712
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    4731..5319
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4784..4804)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5202..5213)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5353..5464
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   join(5582..5691,1..31)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   join(5582..5691,1..20)
                     /label=AAV2 ITR
ORIGIN
        1 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
       61 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
      121 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      181 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      241 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
      301 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
      361 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
      421 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
      481 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
      541 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
      601 agttacgcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      661 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      721 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      781 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      841 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      901 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      961 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1021 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1081 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1141 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     1201 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1261 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1321 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1381 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1441 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1501 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1561 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1621 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1681 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1741 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1801 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1861 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1921 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1981 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2041 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2101 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2161 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2221 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2281 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2341 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2401 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2461 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2521 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2581 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtcc tgcaggcagc
     2641 tgcgcgctcg ctcgctcact gaggccgccc gggcgtcggg cgacctttgg tcgcccggcc
     2701 tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag gggttcctgc
     2761 ggccgcacgc gtttaattaa gtgtctagac tgcagagggc cctgcgtatg agtgcaagtg
     2821 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca
     2881 ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg
     2941 ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct
     3001 tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca
     3061 ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc
     3121 ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg
     3181 cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg
     3241 tcgtgcctga gagcgcagtc gagaaaccgg ctagaggatc cgccaccatg actcccaaca
     3301 gtatgacaga aaatggcctt acagcctggg acaaaccgaa gcactgtcca gaccgagaac
     3361 acgactggaa gctagtagga atgtctgaag cctgcctaca taggaagagc cattcagaga
     3421 ggcgcagcac gttgaaaaat gaacagtcgt cgccacatct catccagacc acttggacta
     3481 gctcaatatt ccatctggac catgatgatg tgaacgacca gagtgtctca agtgcccaga
     3541 ccttccaaac ggaggagaag aaatgtaaag ggtacatccc cagttactta gacaaggacg
     3601 agctctgtgt agtgtgtggt gacaaagcca ccgggtatca ctaccgctgt atcacgtgtg
     3661 aaggctgcaa gggtttcttt agaagaacca ttcagaaaaa tctccatcca tcctattcct
     3721 gtaaatatga aggaaaatgt gtcatagaca aagtcacgcg aaatcagtgc caggaatgtc
     3781 gctttaagaa atgcatctat gttggcatgg caacagattt ggtgctggat gacagcaaga
     3841 ggctggccaa gaggaagctg atagaggaga accgggagaa aagacggcgg gaagagctgc
     3901 agaagtccat cgggcacaag ccagagccca cagacgagga atgggagctc atcaaaactg
     3961 tcaccgaagc ccatgtggcg accaacgccc aaggcagcca ctggaagcaa aaacggaaat
     4021 tcctgccaga agacattgga caagcaccaa tagtcaatgc cccagaaggt ggaaaggttg
     4081 acttggaagc cttcagccat tttacaaaaa tcatcacacc agcaattacc agagtggtgg
     4141 attttgccaa aaagttgcct atgttttgtg agctgccatg tgaagaccag atcatcctcc
     4201 tcaaaggctg ctgcatggag atcatgtccc ttcgcgctgc tgtgcgctat gacccagaaa
     4261 gtgagacttt aaccttgaat ggggaaatgg cagtgacacg gggccagctg aaaaatgggg
     4321 gtcttggggt ggtgtcagac gccatctttg acctgggcat gtctctgtct tctttcaacc
     4381 tggatgacac tgaagtagcc ctccttcagg ccgtcctgct gatgtcttca gatcgcccgg
     4441 ggcttgcctg tgttgagaga atagaaaagt accaagatag tttcctgctg gcctttgaac
     4501 actatatcaa ttaccgaaaa caccacgtga cacacttttg gccaaaactc ctgatgaagg
     4561 tgacagatct gcggatgata ggagcctgcc atgccagccg cttcctgcac atgaaggtgg
     4621 aatgccccac agaactcttc ccccctttgt tcttggaagt gttcgaggat ggtggaggcg
     4681 gaagttaccc atacgatgtt ccagattacg cttagtaaaa gcttatcgat aatcaacctc
     4741 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4801 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4861 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4921 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4981 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5041 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5101 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg
     5161 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5221 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5281 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg
     5341 agagatcttc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     5401 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     5461 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     5521 agggggagga ttgggaagac aatagcaggc atgagatctc acgtgcggac cgagcggccg
     5581 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     5641 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc a
//
