LOCUS       sequence-444355-        2570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0133
FEATURES             Location/Qualifiers
     source          1..2570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2..96)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(117..776)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     691..710
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     938..956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(960..1003)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             1020..1532
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     terminator      1549..1606
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1630..1647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1801..2389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1881..1900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
       61 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
      121 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
      181 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
      241 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
      301 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
      361 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
      421 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
      481 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
      541 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
      601 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
      661 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
      721 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
      781 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
      841 tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt
      901 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa
      961 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt cggtctcaaa
     1021 tggtattcac actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg
     1081 accaagtcct tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa
     1141 ctccgatcca aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca
     1201 tcatcccgta tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg
     1261 tggtgtaccc tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa
     1321 tcgacggggt tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg
     1381 tgttcgacgg caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg
     1441 acgagcgcct gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga
     1501 ccggctggcg gctgtgcgaa cgcattctgg cttcgcgaga ccctgcagtc cggcaaaaaa
     1561 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat
     1621 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1681 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1741 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1801 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1861 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1921 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1981 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2041 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2101 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2161 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2221 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2281 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2341 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2401 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2461 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2521 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg
//
